K. lactis 的蛋白表达。SDS-PAGE 电泳分离检测分泌性表达的重组麦芽糖结合蛋白（MBP），菌体生长在富含蛋白胨（peptone）的培养基中，直接上样检测。考马斯亮蓝染色鉴定。泳道 1：蛋白分子量 Marker；泳道 2：野生型 K. lactis 细胞培养液（15 μl）；泳道 3：整合有大肠杆菌 malE 基因表达框的 K. lactis 细胞培养液（15 μl）。
whatmanPH测试纸2612-990特点： l 直接测定PH值 l 广泛PH测试的常规校准 l 廉价 l 便携式，易于野外使用 CS型条状试纸 这种的中间是用于测PH值的指示剂，两边8个或更多不同颜色片段是这种指示剂对应的比色卡。测定溶液的PH值时，试纸中间的指示剂颜色发生变化，与比色卡上相对应的颜色做比较
8 arm PEG derivatives have multiple reactive groups that can be used to modify proteins, peptides and other materials via their functional groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .
Physical Properties:
Off-white/white solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Fluorescein and biotin labeled lysozyme is a multifunctional fluorescent biotin lysozyme conjugate. Lysozymes are a family of catlytic enzymes which damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. Lysozyme is also known as muramidase or N-acetylmuramide glycanhydrolase. Nanocs provides a variety of chemically functionalized and bioconjugated lysozyme with different functionality. These functional lysozyme products can be used for enzyme studies, bioassay development and many other biomedical research. Nanocs Fluorescein and biotin labeled lysozyme is a green fluorescent lysozyme conjugate that has excitation/emission wavelength at 495/515 nm. Meanwhile, biotin can bind to avidin or streptavidin with high affinity. This fluorescent lysozyme conjugate can be easily detected by fluorescent instruments in FITC channel. All our lysozyme conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
Product Specifications:
Lysozyme source: Hen eggs.
1~4 fluoresceins and biotins on each lysozyme molecule.
Molecular weight: ~14.3 kD
Lysozyme conjugates provided either in water solution or dried powder;
K.lactis的蛋白表达。SDS-PAGE 电泳分离检测分泌性表达的重组麦芽糖结合蛋白（MBP），菌体生长在富含蛋白胨（peptone）的培养基中，直接上样检测。考马斯亮蓝染色鉴定。泳道 1：蛋白分子量 Marker；泳道 2：野生型 K. lactis 细胞培养液（15 μl）；泳道 3：整合有大肠杆菌 malE 基因表达框的 K. lactis细胞培养液（15 μl）。
Desthiobiotin PEG Azide is one of Nanocs’ reactive desthiobiotin derivatives. Azide is a commonly used click chemistry functional group that can react with alkyne catalyzed by copper ion. It can also react with strain-promoted cyclooctyne such as DBCO spontaneously without need any catalyst. Reaction between azide and alkyne allows attachment of destionbiotin tag to targeted molecules or material substrates with high efficiency. Resulted desthiobiotin can bind to avidin or streptavidin, and it can be eluted from avidin/streptavidin complex under milder conditions. PEG linker between azide and desthiobiotin offers higher water solubility, reduced steric hindrance and enhanced activity.
Physical Properties:
Off-white/white solid;
Soluble in aqeous solution, DMSO or DMF;
Reactive group: Azide (-N3);
Reactive to: Alkye, DBCO, etc.;
Storage Conditions:
Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
Off-white/white solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
