上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Azido succinimidyl acetic acid NHS
|
标签归档：nhs
Azido succinimidyl acetic acid NHS Cat. No. AZ3NS-02 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Azido succinimidyl acetic acid NHS
|
DOPE PEG NHS Cat. No. PG2-DONS-3k 3400 Da 25 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
DOPE PEG NHS
|
Description:
1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine (DOPE) conjugated Polyethylene Glycol, DOPE PEG is an unsaturated C18 phospholipid PEG conjugate which has both hydrophilicity and hydrophobility. Pegylated phospholipids are excellent liposome formation materials that can be used for drug delivery, gene transfection and vaccine delivery as well. Pegylation of phospholipids significantly improves the blood circulation time and stability for encapsulated drugs. These materials can also be used for targeted drug delivery by modifying their surfaces with targeting ligands such as antibodies, peptides.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
- SDS
- DataSheet
Alkyne NHS Cat. No. AK1-01 25 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Alkyne NHS
|
Dibenzylcyclooctyne, DBCO NHS Cat. No. DB-NS-01 MW 402.40 5 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Dibenzylcyclooctyne, DBCO NHS
|
Description:
DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Physical Properties:
- Yellow/pale-yellow solid or viscous liquid;
- Soluble in DMSO or DMF;
Storage Conditions:
- Store at -20 0C, desiccate. NHS tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
NHS esters are moisture senstivie. To avoid moisture condensation onto the product always let vial come to room temperature before opening; be exposure to limit exposure to moisture and restore under atmosphere. The NHS ester moiety readily hydrolyzes and becomes non-reactive; therefore, prepare stock solutions immediately before use. Stock solutions in anhydrous solvents can be kept for several days (freeze when not in use). Hydrolysis of the NHS ester is a competing reaction. Conjugation with primary amines of proteins/peptides (i.e., acylation) is favored at near neutral pH (6-9) and with concentrated protein solutions. For conjugation, use non-amine containing buffers at pH 7-9 such as PBS (20 mM sodium phosphate, 150 mM sodium chloride, pH 7.4); 20 mM HEPES; 100 mM carbonate/biocarbonate; or 50 mM borate buffer. Do not use buffers that contain primary amines, (e.g., Tris, glycine). Avoid buffers that contain azides, which can react with DBCO. Dissolve DBCO water miscible organic solvent such as DMSO or DMF before diluting in final reaction buffer. DBCO-NHS ester is not soluble in aqueous buffers.
Materials Required:
- Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
- Water miscible solvents: DMSO or DMF.
- Quenching buffer: 1 M Tris.HCl, pH 8.0.
Reaction Steps:
Prepare proteins in PBS. Immediately before use, prepare 10 mM of the DBCO-NHS reagent in DMSO or DMF. Add the NHS reagent to the protein sample at a final: mM. If For samples <5 mg/ml, use a 20 to 50 fold molar excess. Incubate the reaction at room temperature for 30 minutes or on ice for 2 hours. Stop the reaction by adding Quenching Buffer 100 mM Tris.or on ice for 15 minutes. Remove non reagentby dialysis or desalting.
Copper-free Click Reaction:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
Dibenzylcyclooctyne, DBCO NHS Cat. No. DB-NS-02 MW 402.40 10 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Dibenzylcyclooctyne, DBCO NHS
|
Description:
DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Physical Properties:
- Yellow/pale-yellow solid or viscous liquid;
- Soluble in DMSO or DMF;
Storage Conditions:
- Store at -20 0C, desiccate. NHS tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
NHS esters are moisture senstivie. To avoid moisture condensation onto the product always let vial come to room temperature before opening; be exposure to limit exposure to moisture and restore under atmosphere. The NHS ester moiety readily hydrolyzes and becomes non-reactive; therefore, prepare stock solutions immediately before use. Stock solutions in anhydrous solvents can be kept for several days (freeze when not in use). Hydrolysis of the NHS ester is a competing reaction. Conjugation with primary amines of proteins/peptides (i.e., acylation) is favored at near neutral pH (6-9) and with concentrated protein solutions. For conjugation, use non-amine containing buffers at pH 7-9 such as PBS (20 mM sodium phosphate, 150 mM sodium chloride, pH 7.4); 20 mM HEPES; 100 mM carbonate/biocarbonate; or 50 mM borate buffer. Do not use buffers that contain primary amines, (e.g., Tris, glycine). Avoid buffers that contain azides, which can react with DBCO. Dissolve DBCO water miscible organic solvent such as DMSO or DMF before diluting in final reaction buffer. DBCO-NHS ester is not soluble in aqueous buffers.
Materials Required:
- Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
- Water miscible solvents: DMSO or DMF.
- Quenching buffer: 1 M Tris.HCl, pH 8.0.
Reaction Steps:
Prepare proteins in PBS. Immediately before use, prepare 10 mM of the DBCO-NHS reagent in DMSO or DMF. Add the NHS reagent to the protein sample at a final: mM. If For samples <5 mg/ml, use a 20 to 50 fold molar excess. Incubate the reaction at room temperature for 30 minutes or on ice for 2 hours. Stop the reaction by adding Quenching Buffer 100 mM Tris.or on ice for 15 minutes. Remove non reagentby dialysis or desalting.
Copper-free Click Reaction:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
DBCO-PEG4-NHS Cat. No. DB-NS-EG4-1 5 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
DBCO-PEG4-NHS
|
Description:
DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Physical Properties:
- Yellow/pale-yellow solid or viscous liquid;
- Soluble in DMSO or DMF;
Storage Conditions:
- Store at -20 0C, desiccate. NHS tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
NHS esters are moisture senstivie. To avoid moisture condensation onto the product always let vial come to room temperature before opening; be exposure to limit exposure to moisture and restore under atmosphere. The NHS ester moiety readily hydrolyzes and becomes non-reactive; therefore, prepare stock solutions immediately before use. Stock solutions in anhydrous solvents can be kept for several days (freeze when not in use). Hydrolysis of the NHS ester is a competing reaction. Conjugation with primary amines of proteins/peptides (i.e., acylation) is favored at near neutral pH (6-9) and with concentrated protein solutions. For conjugation, use non-amine containing buffers at pH 7-9 such as PBS (20 mM sodium phosphate, 150 mM sodium chloride, pH 7.4); 20 mM HEPES; 100 mM carbonate/biocarbonate; or 50 mM borate buffer. Do not use buffers that contain primary amines, (e.g., Tris, glycine). Avoid buffers that contain azides, which can react with DBCO. Dissolve DBCO water miscible organic solvent such as DMSO or DMF before diluting in final reaction buffer. DBCO-NHS ester is not soluble in aqueous buffers.
Materials Required:
- Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
- Water miscible solvents: DMSO or DMF.
- Quenching buffer: 1 M Tris.HCl, pH 8.0.
Reaction Steps:
Prepare proteins in PBS. Immediately before use, prepare 10 mM of the DBCO-NHS reagent in DMSO or DMF. Add the NHS reagent to the protein sample at a final: mM. If For samples <5 mg/ml, use a 20 to 50 fold molar excess. Incubate the reaction at room temperature for 30 minutes or on ice for 2 hours. Stop the reaction by adding Quenching Buffer 100 mM Tris.or on ice for 15 minutes. Remove non reagentby dialysis or desalting.
Copper-free Click Reaction:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
DBCO-PEG2000-NHS Cat. No. DB-NS-P2k 25 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
DBCO-PEG2000-NHS
|
Description:
DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Physical Properties:
- Yellow/pale-yellow solid or viscous liquid;
- Soluble in DMSO or DMF;
Storage Conditions:
- Store at -20 0C, desiccate. NHS tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
NHS esters are moisture senstivie. To avoid moisture condensation onto the product always let vial come to room temperature before opening; be exposure to limit exposure to moisture and restore under atmosphere. The NHS ester moiety readily hydrolyzes and becomes non-reactive; therefore, prepare stock solutions immediately before use. Stock solutions in anhydrous solvents can be kept for several days (freeze when not in use). Hydrolysis of the NHS ester is a competing reaction. Conjugation with primary amines of proteins/peptides (i.e., acylation) is favored at near neutral pH (6-9) and with concentrated protein solutions. For conjugation, use non-amine containing buffers at pH 7-9 such as PBS (20 mM sodium phosphate, 150 mM sodium chloride, pH 7.4); 20 mM HEPES; 100 mM carbonate/biocarbonate; or 50 mM borate buffer. Do not use buffers that contain primary amines, (e.g., Tris, glycine). Avoid buffers that contain azides, which can react with DBCO. Dissolve DBCO water miscible organic solvent such as DMSO or DMF before diluting in final reaction buffer. DBCO-NHS ester is not soluble in aqueous buffers.
Materials Required:
- Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
- Water miscible solvents: DMSO or DMF.
- Quenching buffer: 1 M Tris.HCl, pH 8.0.
Reaction Steps:
Prepare proteins in PBS. Immediately before use, prepare 10 mM of the DBCO-NHS reagent in DMSO or DMF. Add the NHS reagent to the protein sample at a final: mM. If For samples <5 mg/ml, use a 20 to 50 fold molar excess. Incubate the reaction at room temperature for 30 minutes or on ice for 2 hours. Stop the reaction by adding Quenching Buffer 100 mM Tris.or on ice for 15 minutes. Remove non reagentby dialysis or desalting.
Copper-free Click Reaction:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
Vitamin E PEG NHS, Tocopherol PEG NHS Cat. No. PG2-NSVE-5k 5000 Da 5 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Vitamin E PEG NHS, Tocopherol PEG NHS
|
Vitamin E PEG NHS, MW 2000, 3400, 5000, 10000
Description:
Vitamin E, also called tocopherols, are a series of chiral organic molecules that vary in their degree of methylation of the phenol moiety of the chromanol ring. Tocopherols are lipid soluble anti-oxidants that protect cell membrane from xoidative damage. Vitamin E PEG NHS offered by Nanocs is a class of bioactive PEGs made from the PEG conjugation to α-tocopherol that can be used to for drug delivery or bioassay development. This product is amine reactive and can react with primary amine groups at pH 8-10.
Physical Properties:
- White solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
- Molecule structure:
Storage Conditions:
- Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
- SDS
- DataSheet
Vitamin E PEG NHS, Tocopherol PEG NHS Cat. No. PG2-NSVE-3k 3400 Da 5 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Vitamin E PEG NHS, Tocopherol PEG NHS
|
Vitamin E PEG NHS, MW 2000, 3400, 5000, 10000
Description:
Vitamin E, also called tocopherols, are a series of chiral organic molecules that vary in their degree of methylation of the phenol moiety of the chromanol ring. Tocopherols are lipid soluble anti-oxidants that protect cell membrane from xoidative damage. Vitamin E PEG NHS offered by Nanocs is a class of bioactive PEGs made from the PEG conjugation to α-tocopherol that can be used to for drug delivery or bioassay development. This product is amine reactive and can react with primary amine groups at pH 8-10.
Physical Properties:
- White solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
- Molecule structure:
Storage Conditions:
- Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
- SDS
- DataSheet
Vitamin E PEG NHS, Tocopherol PEG NHS Cat. No. PG2-NSVE-2k 2000 Da 5 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Vitamin E PEG NHS, Tocopherol PEG NHS
|
Vitamin E PEG NHS, MW 2000, 3400, 5000, 10000
Description:
Vitamin E, also called tocopherols, are a series of chiral organic molecules that vary in their degree of methylation of the phenol moiety of the chromanol ring. Tocopherols are lipid soluble anti-oxidants that protect cell membrane from xoidative damage. Vitamin E PEG NHS offered by Nanocs is a class of bioactive PEGs made from the PEG conjugation to α-tocopherol that can be used to for drug delivery or bioassay development. This product is amine reactive and can react with primary amine groups at pH 8-10.
Physical Properties:
- White solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
- Molecule structure:
Storage Conditions:
- Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
- SDS
- DataSheet
OPSS-PEG-NHS, SPDP-PEG-NHS Cat. No. PG2-NSOS-5k 5000 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
OPSS-PEG-NHS, SPDP-PEG-NHS
|
Description:
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
- SDS
- DataSheet
OPSS-PEG-NHS, NHS-PEG-SPDP Cat. No. PG2-NSOS-3k 3400 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
OPSS-PEG-NHS, NHS-PEG-SPDP
|
Description:
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
- SDS
- DataSheet
OPSS-PEG-NHS, PDP-PEG-NHS Cat. No. PG2-NSOS-2k 2000 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
OPSS-PEG-NHS, PDP-PEG-NHS
|
Description:
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
- SDS
- DataSheet
OPSS-PEG-NHS, NHS-PEG-PDP Cat. No. PG2-NSOS-10k 10000 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
OPSS-PEG-NHS, NHS-PEG-PDP
|
Description:
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
- SDS
- DataSheet
OPSS-PEG-NHS, PDP-PEG-NHS Cat. No. PG2-NSOS-1k 1000 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
OPSS-PEG-NHS, PDP-PEG-NHS
|
Description:
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
- SDS
- DataSheet
OPSS-PEG-NHS, NHS-PEG-PDP Cat. No. PG2-NSOS-20k 20000 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
OPSS-PEG-NHS, NHS-PEG-PDP
|
Description:
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
- SDS
- DataSheet
Succinimidyl acid PEG, mPEG-NHS Cat. No. PG1-SPA-5k 5000 Da 1 g修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Succinimidyl acid PEG, mPEG-NHS
|
Description:
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
- Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
- PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
- Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjugates can be purified either by size exclusion chromatography or dialysis.
- SDS
- DataSheet
Silane-PEG-NHS Cat. No. PG2-NSSL-5k 5000 Da 100 mg修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Silane-PEG-NHS
|
Description:
Ethoxy or methoxyl silane functionalized polyethylene glycol, silane PEG (PEG-Si) is a surface reactive PEG derivative that can be used to modify glass, silica and other surfaces via the reaction between hydroxyl group and ethoxyl/methoxyl silane. Pegylation can greatly suppress the non-specific binding of charged molecules to the modified surfaces. And they have wide applications for medica device, biomems or biocompatible material development.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, desiccated. Silane PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
Generally, silane PEG is first dissolved in a mixture of ethanol/water solution, then allow the solution to contact with oxidized glass or silica surface for 30 min to 2 hours. Under this condition, silane PEG should bind to the material covalently.
Materials Required:
- Pegylation solution: Ethanol/water (w/w, 95%/5%);
- Silane PEG stock solution, 10~50 mg/mL in pegylation solution, prepare in fresh.
- Washing solution: Distilled water.
Reaction Steps:
1. Dissolve targeted materials in Pegylation buffer.
2. Add silane PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG needed for optimal conjugation;
3. Allow mixture agitates at room temperature for 30 min at room temperature or 2 hours at 4 -20 0C.
4. Wash out unreacted materials either by distilled water.
- SDS
- DataSheet
Succinimidyl acid PEG, mPEG-NHS Cat. No. PG1-SPA-2k 2000 Da 1 g修饰性聚乙二醇
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
Succinimidyl acid PEG, mPEG-NHS
|
Description:
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
Physical Properties:
- Off-white/white solid or viscous liquid depends on molecule weight;
- Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
- Store at -20 0C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
- Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
- PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
- Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjugates can be purified either by size exclusion chromatography or dialysis.
- SDS
- DataSheet