BD细胞凋亡试剂盒FITC Annexin V Apoptosis Detection Kit IBD556547

BD细胞凋亡试剂盒FITC Annexin V Apoptosis Detection Kit I

简要描述:
ContentsAnnexin V-FITC, Propidium Iodide Staining Solution, Annexin V Binding BufferSize100 TestsRegulatory Status RUO
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 Description

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The

apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment,

condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features.

In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma

membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding

protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC.

This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing

apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, FITC Annexin V staining can identify apoptosis at an earlier

stage than assays based on nuclear changes such as DNA fragmentation.

FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either

apoptotic or necrotic processes. Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium

iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, FITC Annexin V

positive). Viable cells with intact membranes exclude PI, wheras the membranes of dead and damaged cells are permeable to PI. For example,

cells that are considered viable are FITC Annexin V and PI negative; cells that are in early apoptosis are FITC Annexin V positive and PI

negative; and cells that are in late apoptosis or already dead are are both FITC Annexin V and PI positive. This assay does not distinguish

between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead

cells will stain with both FITC Annexin V and PI. However, when apoptosis is measured over time, cells can be often tracked from FITC

Annexin V and PI negative (viable, or no measurable apoptosis), to FITC Annexin V positive and PI negative (early apoptosis, membrane

integrity is present) and finally to FITC Annexin V and PI positive (end stage apoptosis and death). The movement of cells through these three

stages suggests apoptosis. In contrast, a single observation indicating that cells are both FITC Annexin V and PI positive, in of itself, reveals

less information about the process by which the cells underwent their demise.

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

556547 Rev. 5 Page 1 of 3

Flow Cytometric Analysis of FITC Annexin V staining. Jurkat cells

(Human T-cell leukemia; ATCC TIB-152) were left untreated (top

panels) or treated for 4 hours with 12 μM campotothecin (bottom

panels). Cells were incubated with FITC Annexin V in a buffer

containing propidium iodide (PI) and analyzed by flow cytometry.

Untreated cells were primarily FITC Annexin V and PI negative,

indicating that they were viable and not undergoing apoptosis. After a

4 hour treatment (bottom panels), there were primarily two

populations of cells: Cells that were viable and not undergoing

apoptosis (FITC Annexin V and PI negative) and cells undergoing

apoptosis (FITC Annexin V positive and PI negative). A minor

population of cells were observed to be FITC Annexin V and PI

positive, indicating that they were in end stage apoptosis or already

dead.

Application Notes

Application

Flow cytometry Routinely Tested

Recommended Assay Procedure:

FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with

a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium

and salt concentrations are required for optimal staining as described in the FITC Annexin V Staining Protocol. Investigators should note that

FITC Annexin V flow cytometric analysis on adherent cell types (e.g HeLa, NIH 3T3, etc.) is not routinely tested as specific membrane

damage may occur during cell detachment or harvesting. Methods for utilizing Annexin V for flow cytometry on adherent cell types,

however, have been previously reported (Casiola-Rosen et al. and van Engelend et al.).

INDUCTION OF APOPTOSIS BY CAMPTOTHECIN

The following protocol is provided as an illustration on how FITC Annexin V may be used on a cell line (Jurkat).

Materials

1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO.

2. Jurkat T cells (ATCC TIB-152).

Procedure

1. Add Camptothecin (final conc. 4-6 μM) to 1 x 10^6 Jurkat cells.

2. Incubate the cells for 4-6 hr at 37°C.

3. Proceed with the FITC Annexin V Staining Protocol to measure apoptosis.

FITC ANNEXIN V STAINING PROTOCOL

FITC Annexin V is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on

the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid

phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external

environment. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying

apoptotic cells with exposed PS. Propidium Iodide (PI) is a standard flow cytometric viability probe and is used to distinguish viable from

nonviable cells. Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. Cells that

stain positive for FITC Annexin V and negative for PI are undergoing apoptosis. Cells that stain positive for both FITC Annexin V and PI are

either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both FITC Annexin V and PI are

alive and not undergoing measurable apoptosis.

556547 Rev. 5 Page 2 of 3

Reagents

1. FITC Annexin V (component no. 51-65874X): Use 5 μl per test.

2. Propidium Iodide (PI) (component no. 51-66211E) is a convenient, ready-to-use nucleic acid dye. Use 5 μl per test.

3. 10X Annexin V Binding Buffer (component no. 51-66121E): 0.1 M Hepes/NaOH (pH 7.4), 1.4 M NaCl, 25 mM CaCl2. For a 1X working

solution, dilute 1 part of the 10X Annexin V Binding Buffer to 9 parts of distilled water.

Staining

1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml.

2. Transfer 100 μl of the solution (1 x 10^5 cells) to a 5 ml culture tube.

3. Add 5 μl of FITC Annexin V and 5 μl PI.

4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.

5. Add 400 μl of 1X Binding Buffer to each tube. Analyze by flow cytometry within 1 hr.

SUGGESTED CONTROLS FOR SETTING UP FLOW CYTOMETRY

The following controls are used to set up compensation and quadrants:

1. Unstained cells.

2. Cells stained with FITC Annexin V (no PI).

3. Cells stained with PI (no FITC Annexin V).

Other Staining Controls:

A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining with FITC Annexin V and/or FITC

Annexin V and PI. It is important to note that the basal level of apoptosis and necrosis varies considerably within a population. Thus, even in the

absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis (FITC Annexin V

positive, PI negative or FITC Annexin V positive, PI positive).

The untreated population is used to define the basal level of apoptotic and dead cells. The percentage of cells that have been induced to undergo

apoptosis is then determined by subtracting the percentage of apoptotic cells in the untreated population from percentage of apoptotic cells in the

treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged

membrane and stain positive for PI as well as for FITC Annexin V. Thus the assay does not distinguish between cells that have already undergone

an apoptotic cell death and those that have died as a result of necrotic pathway, because in either case the dead cells will stain with both FITC

Annexin V and PI.

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before

discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.

4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

References

Andree HA, Reuingsperger CP, Hauptmann R, Hemker HC, Hermens WT, Willems GM. Binding of vascular anticoagulant alpha (VAC alpha) to planar

phospholipid bilayers. J Biol Chem. 1990; 265(9):4923-4928. (Biology)

Casciola-Rosen L, Rosen A, Petri M, Schlissel M. Surface blebs on apoptotic cells are sites of enhanced procoagulant activity: implications for coagulation events

and antigenic spread in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1996; 93(4):1624-1629. (Biology)

Homburg CH, de Haas M, von dem Borne AE, Verhoeven AJ, Reuingsperger CP, Roos D. Human neutrophils lose their surface Fc gamma RIII and acquire

Annexin V binding sites during apoptosis in vitro. Blood. 1995; 85(2):532-540. (Biology)

Koopman G, Reuingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH. Annexin V for flow cytometric detection of phosphatidylserine expression on

B cells undergoing apoptosis. Blood. 1994; 84(5):1415-1420. (Biology)

Martin SJ, Reuingsperger CP, McGahon AJ, et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of

the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med. 1995; 182(5):1545-1556. (Biology)

O'Brien MC, Bolton WE. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow

cytometry. Cytometry. 1995; 19(3):243-255. (Biology)

Raynal P, Pollard HB. Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins.

Biochim Biophys Acta. 1994; 1197(1):63-93. (Biology)

Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence

in single laser flow cytometry. Cytometry. 1992; 13(2):204-208. (Biology)

van Engeland M, Ramaekers FC, Schutte B, Reuingsperger CP. A novel assay to measure loss of plasma membrane asymmetry during apoptosis of adherent

cells in culture. Cytometry. 1996; 24(2):131-139. (Biology)

Vermes I, Haanen C, Steffens-Nakken H, Reuingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early

apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995; 184(1):39-51. (Biology)

556547

 

FITC-Annexin V/PI 细胞凋亡试剂盒 货号: F6012S/F6012M/F6012L 规格: 10T/50T/100T

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

FITC-Annexin V/PI 细胞凋亡试剂盒

产品货号: F6012S/F6012M/F6012L

产品规格: 10T/50T/100T

目录价(元):220/737/1244

推荐仪器:流式细胞仪(主推)、荧光显微镜

大包装询价


产品概述:

产品内容:

组分

  F6012S10T

  F6012M50T

  F6012L100T

   A. 1× Annexin V 结合缓冲液

10 mL

50 mL

50 mL×2

B. FITC-Annexin V

50 μL

250 μL

500 μL

C. PI

100 μL

500 μL

1 mL

储存条件
4℃避光冷藏,请勿冻存。有效期见外包装。

光谱特性
FITC-Annexin V: Ex/Em = 494/518 nm
PI: Ex/Em = 535/617 nm (with DNA)

产品介绍

FITC-Annexin V/PI凋亡试剂盒提供了一种快速简便的方法,通过标记早期凋亡细胞(绿色)和坏死或晚期凋亡的细胞(红色)检测细胞凋亡水平。产品可以使用流式细胞仪或其它荧光检测设备进行检测。
Annexin V(膜联蛋白-V)是一种分子量为35-36KD的Ca2+依赖性磷脂结合蛋白,可于磷脂酰丝氨酸(PS)选择性结合。磷脂酰丝氨酸(PS)主要分布在细胞膜内侧,即与细胞浆相邻的一侧。在细胞发生凋亡的早期,不同类型的细胞都会把磷脂酰丝氨酸外翻到细胞表面,暴露在细胞外环境中。此时,使用绿色荧光探针FITC标记的Annexin V,即FITC – Annexin V,与外翻的磷脂酰丝氨酸(PS)结合,就可用流式细胞仪或荧光显微镜直接检测到磷脂酰丝氨酸的外翻这一细胞凋亡的重要特征。对于坏死或晚期凋亡的细胞,由于细胞完整性已经被破坏,FITC – Annexin V则可以进入胞浆与处于磷脂层内侧的PS结合,从而也使坏死细胞呈现绿色荧光。
碘化丙啶(Propidium Iodide, PI)是一种DNA结合染料,它可以染色坏死细胞或凋亡晚期丧失细胞膜完整性的细胞的细胞核。PI可以由488、532或546 nm的激光激发,呈现红色荧光。

注意事项
1. 使用前请将产品瞬时离心至管底,再进行后续实验。
2. 为降低细胞凋亡进程,孵育过程可在冰上操作,但孵育时间至少延长至30 min。
3. 由于细胞凋亡是一个快速的过程,建议样品在染色后 1 h之内进行分析。
4. 对于贴壁细胞,消化是一个关键步骤。贴壁细胞诱导细胞凋亡时如有漂浮细胞,需收集漂浮细胞和贴壁细胞后合并染色。处理贴壁细胞时要小心操作,尽量避免人为的损伤细胞。胰酶消化时间过短,细胞需要用力吹打才能脱落,容易造成细胞膜的损伤,PI 摄入过多;消化时间过长,细胞膜同样易造成损伤,甚至会影响细胞膜上磷脂酰丝氨酸与 FITC-Annexin V的结合。消化时将胰酶铺满孔板底后,轻摇时胰酶与细胞充分接触,然后倒掉大部分胰酶,利用剩余的少量胰酶再消化一段时间,待细胞间空隙增大,瓶底呈花斑状即可终止。在消化液中尽量不用 EDTA,EDTA 会影响 Annexin V 与 PS 的结合。
5. 贴壁细胞用胰蛋白酶消化后,建议在最佳培养条件和培养基中恢复约30 min后染色,避免假阳性。
6. 为了避免洗涤细胞时损失细胞,在吸液时可以用大的 Tip 头套上小的 Tip 头吸液。
7. 染料的最佳使用浓度由具体实验要求确定。
8. 荧光染料均存在淬灭问题,保存和使用过程中请尽量注意避光,以减缓荧光淬灭。
9. 为了您的安全和健康,请穿实验服并戴一次性手套操作。


说明书:

FITC-Annexin V/PI 细胞凋亡试剂盒 货号:               F6012S/F6012M/F6012L  规格:               10T/50T/100T UE-F6012S/F6012M/F6012L    
MSDS:

MSDS F6012 FITC-Annexin V&PI
常见问题解答:

为何染色结果显示细胞凋亡率过高?

1. 凋亡时间处理过长,使细胞全部死亡。建议重新摸索细胞凋亡条件,使细胞处于不同的凋亡状态。
2. 对于贴壁细胞,胰蛋白酶消化时间过长或机械刮擦细胞会暂时破坏质膜,使Annexin V能够与细胞膜胞内面上的磷脂酰丝氨酸结合,导致假阳性染色。建议在胰蛋白酶消化或机械刮擦细胞后,让细胞在最佳细胞培养条件和培养基中培养约30分钟,以恢复其膜完整性。

实验结果使用什么仪器观察?
实验结果可以通过流式细胞仪和荧光显微镜进行观察,但更推荐使用流式细胞仪。因为正常的细胞和凋亡的细胞膜上都有磷脂酰丝氨酸(PS),只是凋亡细胞膜外更多,可以结合更多的Annexin V,流式细胞仪更灵敏,可以区分出微小差异,将正常细胞与凋亡细胞分成不同的两群,但如果用荧光显微镜,可能观察不到这一差别,尤其是贴壁细胞。若要在成像测定中检测细胞凋亡,推荐使用SuperView™ 488 Caspase-3 Assay Kit for Live Cells。

流式细胞仪检测细胞凋亡,结果图中的各个象限代表什么状态的细胞?
左上角:Annexin V-/PI+,机械损伤细胞;右上角: AnnexinV+/PI+,晚期凋亡细胞或坏死;右下角:AnnexinV+/PI-,早期凋亡细胞;左下角:AnnexinV-/PI-,正常细胞。

这款试剂盒可同时检测出凋亡率和细胞周期分布吗?
不可以。检测细胞凋亡时PI结合的是核酸,包括DNA和RNA。而PI用于检测细胞周期时需要只结合DNA,由于RNA的干扰,用细胞凋亡检测试剂盒测出来的细胞周期不准。同时,凋亡检测与周期检测时PI的工作浓度以及样本处理都不一样。 如果需要进行细胞周期检测,推荐Cell Cycle and Apoptosis Kit(C6031)

各象限代表的细胞状态问题?
Q1:(AnnexinV-染料)-/PI+,此区域的细胞为坏死细胞。也可能有少数的晚期凋亡细胞在其中,甚至机械损伤的细胞也包含其中。Annexin V-/PI+越多,实验结果越不可信,建议实验中尽量控制这一象限细胞比例。
Q2: (AnnexinV+染料)+/PI+,此区域的细胞为晚期凋亡细胞或坏死。
Q3:(AnnexinV-染料)+/PI-,此区域的细胞为早期凋亡细胞。
Q4:(AnnexinV-染料)-/PI-,此区域的细胞为活细胞。

FITC-Annexin V/PI 细胞凋亡试剂盒 货号:               F6012S/F6012M/F6012L  规格:               10T/50T/100T


假阳性问题?

1. 消化液中含有EDTA或消化时间过长引起的假阳性。Annexin V和PS的结合需要Ca2+,而EDTA是Ca2+螯合剂,使用含有EDTA的胰酶会造成假阴性。其次消化时间不宜过长,吹打细胞要轻柔,因为胰酶的过度消化和机械损伤都会引起细胞膜的损伤,造成假阳性。细胞消化下来后,建议在培养箱中在孵育30min,使细胞膜恢复完整性,避免假阳性,或者将细胞报讯在含2% BSA的PBS中,防止进一步的损伤。
2. 如果样品来源于血液,必须去除血液中的血小板,因为血小板含有PS,能与Annexin V结合,干扰实验结果。建议:可以含有EDTA的缓冲液200g离心去除血小板, 最后细胞多洗几遍,减小EDTA螯合钙离子产生的影响。
3. 神经细胞膜容易破坏外翻,导致假阳性,所以Annexin V/PI 双染法流式检测细胞凋亡不适用于神经细胞。
4. 诱导时间过长。一般情况下诱导几个小时就可以出现早期凋亡,因此通常不需要处理大于48小时以上,诱导时间过长会使营养物质耗尽,导致细胞状态差,假阳性结果偏高。

荧光染料选择的问题?
1. 实验样本是否带荧光,因为感染病毒或质粒转染的细胞往往带GFP荧光,GFP与YF488/FITC通道重叠,不能选择YF488/FITC标记的Annexin V,此时如果只有488 nm一根激光管可以用PE标记Annexin V,用7-AAD代替PI,若有633 nm激光管,可以改用YF647 Annexin V代替YF488。
2. 如果流式细胞仪带有488 nm和633 nm两个或以上激光管,首先考虑用APC或YF647标记 Annexin V,因为它和PI通道几乎不用考虑光重叠问题,可以减少调节补偿的烦恼,如果只有488 nm激光管则可选择YF488/FITC标记的Annexin V。
3. 处理因素是否带有荧光,本实验室就碰到过一个促凋亡药物Doxorubicin本身发红色荧光,对PI通道的检测造成严重干扰,而且浓度越大干扰越明显。这时建议选用A6079或Y6102 。

圈门问题?
1.要特别注意在FSC/SSC圈门时,所选细胞范围会对结果造成较大影响,所以在分析数据时,要把所有细胞都圈进FSC/SSC的门内,这样实验结果才会更可信。如图,使细胞群大概位于对角线上,并且细胞群较集中,群内细胞比例要在80%以上,如果细胞全部圈入,细胞比例才50%或者更低,说明细胞被压在了坐标轴上,这时要重新调节FSC和SSC。
2.贴壁细胞做Annexin V凋亡检测,通常分群不太规则,这时我们可以根据不加药细胞来画不规则的十字门。如上面图形的设门方式:
FITC-Annexin V/PI 细胞凋亡试剂盒 货号:               F6012S/F6012M/F6012L  规格:               10T/50T/100TFITC-Annexin V/PI 细胞凋亡试剂盒 货号:               F6012S/F6012M/F6012L  规格:               10T/50T/100T

免疫染色与凋亡染色顺序问题?

Annexin V凋亡染色可以和抗体一起对细胞进行染色吗?
可以,但建议Annexin V和其它抗体分开染色。例如Annexin V、PI和CD3、CD8同时标记淋巴细胞,可以先在PBS中标记CD3/CD8后洗去,再在Binding buffer(含钙离子)中标记Annexin V。

这款试剂盒可同时检测出凋亡率和细胞周期分布吗?
不可以。检测细胞凋亡时PI结合的是核酸,包括DNA和RNA。而PI用于检测细胞周期时需要只结合DNA,由于RNA的干扰,用细胞凋亡检测试剂盒测出来的细胞周期不准。同时,凋亡检测与周期检测时PI的工作浓度以及样本处理都不一样。 若果需要进行细胞周期检测,推荐Cell Cycle and Apoptosis Kit(C6031)

固定、冷冻、切片或解离的组织,还可以做流式细胞分析吗?
不建议做流式细胞分析。因为流式细胞分析要求细胞分散、单个,活性好,这些状态下的样本外膜上的PS及膜的完整性都受到了影响,因此无法依赖膜的完整性区分健康细胞和凋亡细胞。组织在冻存、复温的过程中会有大量的细胞死亡,同时经过这一过程的组织在分离成单个细胞的时候会形成许多细胞碎片,不宜上流式检测,所以用于流式检测的标本最好是新鲜标本,即取材后立即检测,效果最好。 液氮保存的组织块,可以将组织标本进行切片,然后做原位染色,观察组织细胞的凋亡。

Annexin V可以用在植物和细菌中吗?
Annexin V也可以用在植物和细菌中检测凋亡,具体实验步骤可参考相关文献。
使用本产品的文献:

1.MTP18 overexpression contributes to tumor growth and metastasis and associates with poor survival in hepatocellular carcinoma
Zhang, Yu Li, Hui Chang, Hulin Du, LixUE  Hai, Jun Geng, Xilin Yan, Xiang
cell death and disease(2018) 
应用方向:流式细胞仪检测:SMMC7721和 HLF 细胞的凋亡

2.
MS-275对食管鳞癌KYSE-70细胞存活、凋亡、细胞周期以及迁移的影响
刘腾飞,周建康,黄团结,王亚苹,周馨魁,张 乐,张璐玉,陈一豪,刘红涛,关方霞,马珊珊
郑州大学学报(医学版)(2018) 5 547-552
应用方向:流式细胞仪检测:食管鳞癌KYSE-70细胞的凋亡

3.
Histone deacetylases inhibitor MS‐275 suppresses human esophageal squamous cell carcinoma cell growth and progression via the PI3K/Akt/mTOR pathway
Shanshan Ma  Tengfei Liu  Ling Xu  Yaping Wang  Jiankang Zhou  Tuanjie Huang  Peng Li  Hongtao Liu Yanting Zhang  Xinkui Zhou  Yuanbo Cui  Xingxing Zang  Yuming Wang  Fangxia Guan
Journal of Cellular Physiology (23 May 2019)
应用方向:细胞凋亡检测:食管鳞状癌细胞(ESCC)

4.
SDHC-related deficiency of SDH complex activity promotes growth and metastasis of hepatocellular carcinoma via ROS/NFκB signaling
JibinLi,NingLiang,XiaoyuLong,JingZhao,JinYang,XiaohongDu,TaoYang,PengYuan,XiaojunHuang,JianshengZhang,XianliHe,JinliangXing
Cancer Letters Volume 461, 1 October 2019, Pages 44-55
应用方向:细胞凋亡检测::肝细胞癌(HCC)

5.
MTFP1 overexpression promotes the growth of oral squamous cell carcinoma by inducing ROS production
Tingying Xiao,Jian Sun,Zhankui Xing,Fuqiang Xie,Lan Yang,Wenjuan Ding
CELL BIOLOGY INTERNATIONAL
应用方向:细胞凋亡检测:口腔鳞状细胞(OSCC)

6.ALG-2 couples T cell activation and apoptosis by regulating proteasome activity and inflUEncing MCL1 stability
Tian-Sheng He, Wangsheng Ji, Junqi Zhang, Jing Lu,Xinqi Liu 
Cell Death & Disease(11, Article number: 5 (2020))

7.Over‐expression of TFB2M facilitates cell growth and metastasis via activating ROS‐Akt‐NF‐κB signaling in hepatocellular carcinoma
Xilin Geng,Zhimin Gen,Hui Li,Yu Zhang,Jibin Li,Hulin Chang
liver international

8.CRIF1 overexpression facilitates tumor growth and metastasis through inducing ROS/NFκB pathway in hepatocellular carcinoma
Hulin Chang,Juntang Li,Kai Qu,Yong Wan,Sinan Liu,Wei Zheng,Zhiyong Zhang,Chang Liu
 Cell Death & Disease volume 11, Article number: 332 (2020) 

9.Caffeine Targets G6PDH to Disrupt Redox Homeostasis and Inhibit Renal Cell Carcinoma Proliferation
Huanhuan Xu,Lihong Hu, Titi Liu1, Fei Chen, Jin Li, Jing Xu, Li Jiang, Zemin Xiang, Xuanjun Wang,Jun Sheng
Frontiers in Cell and Developmental Biology

10.A Network Pharmacology Approach to Investigate the Anticancer Mechanism and Potential Active Ingredients of Rheum palmatum L. Against Lung Cancer via Induction of Apoptosis
Qing Zhang, Jia Liu, Ruolan Li, Rong Zhao, Mengmeng Zhang, Shujun Wei, Dong Ran, Wei Jin, Chunjie Wu
Frontiers in Pharmacology

11.MIEF2 over-expression promotes tumor growth and metastasis through reprogramming of glucose metabolism in ovarian cancer
Shuhua Zhao, Xiaohong Zhang, Yuan Shi, Lu Cheng, Tingting Song, Bing Wu, Jia Li & Hong Yang
Journal of Experimental & Clinical Cancer Research

12.Repurposing Erythrocytes as a “Photoactivatable Bomb”: A General Strategy for Site-Specific Drug Release in Blood Vessels
Ya-Xuan Zhu,Hao-Ran Jia,Yuxin Guo,Xiaoyang Liu,Ningxuan Zhou,Peidang Liu,Fu-Gen Wu
Small

13.Multienzyme-like Reactivity Cooperatively Impairs Glutathione Peroxidase 4 and Ferroptosis Suppressor Protein 1 Pathways in Triple-Negative Breast Cancer for Sensitized Ferroptosis Therapy
Ke Li, Chuanchuan Lin, Menghuan Li, Kun Xu, Ye He, Yulan Mao, Lu Lu, Wenbo Geng, Xuemin Li, Zhong Luo, Kaiyong Cai
ACS Nano

14.A multiple functional supramolecular system for synergetic treatments of hepatocellular carcinoma
LijingSun, LiyuanChen, KeYang, Wei FengDai, YeYang, XiumingCui, BoYang, ChengxiaoWang
International Journalof Pharmaceutics

FITC,异构体I 货号: F5027 规格: 10 mg

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

FITC,异构体I

产品货号: F5027

产品规格: 10 mg

目录价(元):218

大包装询价


产品概述:

产品参数
外观:可溶于DMSO或DMF的橙黄色固体
Ex/Em:492/520 nm
CAS号:3326-32-7
分子式:C21H11NO5S
分子量:389.4
Cf(A280):0.254
Extinction coefficient(ε):73,000 cm-1M-1
分子结构图:

 FITC,异构体I 货号:               F5027  规格:               10 mg

储存条件
-20℃避光保存,有效期见外包装。

产品介绍

FITC(异构体I)是一种具有胺反应活性的荧光素衍生物,可以用来标记蛋白或其他分子。

注意事项

1. 使用前请将产品瞬时离心至管底,再进行后续实验。
2. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。


说明书:

FITC,异构体I 货号:               F5027  规格:               10 mg UE-F5027    
MSDS:

MSDS F5027 FITC,isomer I

Tetrazine PEG Fluorescein, Tetrazine-PEG-FITC Cat. No. PG2-FCTZ-5k 5000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Tetrazine PEG Fluorescein, Tetrazine-PEG-FITC

Cat. No. PG2-FCTZ-5k
Specification 5000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Tetrazine PEG Derivatives

Description:

Tetrazine PEG derivatives are used to react with TCO (transcyclooctene) groups. The reaction of tetrazine and TCO is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution fast and efficiently. Tetrazine PEG derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

  • Appearance: Yellow to orange solid;
  • Solubility: Soluble in water, ethanol, DMSO, etc.;
  • Reactivity: Tetrazine reacts with TCO.

Storage:

  • Store at -20 0C; Desiccate; Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Tags: tetrazine PEG,

Streptavidin, FITC labeled Cat. No. SV1-FC-1 1 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Streptavidin, FITC labeled

Cat. No. SV1-FC-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Fluorescein streptavidin is a fluorescent streptavidin conjuate that has strong green fluorescent upon excitated at 488 nm. Nanocs provides a variety of chemically functionalized and bioconjugated streptavidin with different functionality. These functional streptavidin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 1~5 fluorescein groups on each streptavidin molecule.
  • streptavidin conjugates provided either in water solution or dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.
Related Products
SV1-RPE-1 , Streptavidin, R-phycoerythrin
SV1-DM-1 , DMPE Streptavidin Conjugate

Heparin, FITC labeled Cat. No. HRN1-FC-1 1 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Heparin, FITC labeled

Cat. No. HRN1-FC-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated Heparin with different functionality. These functional Heparin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Heparin molecule.
  • Heparin conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store4 0C, desiccated Protect from light.

Lipopolysacharides, FITC conjugate Cat. No. LPS-FC-1 1 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Lipopolysacharides, FITC conjugate

Cat. No. LPS-FC-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Lipopolysaccharide, Fluorescein Labeled

Description:

Nanocs offers several fluorescent conjugates of lipopolysaccharides (LPS) from Escherichia coli and Salmonella minnesota. LPS, also called endotoxins, are complex macromolecules present on the outer cell walls of gram-negative bacteria. Our fluoresent Lipopolysaccharide conjugates allow researchers to follow binding, transport and cell internalization processes of LPS. We provides a variety of fluorescently labeled and other functional LPS conjugates, including Fluorescein (FITC or FAM) LPS, Rhodamine LPS (Rhodamine B, Tetetramethyl rhodamine, TMR, TAMRA, etc), Cy3 labeld LPS, Cy5 labeled LPS, Biotin labeled LPS and many other multifunctional LPS products. Fluorescein LPS is a green fluorescein LPS conjugate which give strong fluorescent signal at 515 nm upon excitated around 490 nm. If you have needs for any special LPS products not listed here, please call us and let we make for you!

Specifications:

  • 1~5 fluorescein molecules on each LPS molecule.
  • Lipopolysaccharide conjugates provided either in water solution and dried powder;
  • Excitation/Emission wavelength: 490 nm/515 nm.

Storage Conditions:

  • Store at 4 0C. Protect from light.

Tags: LPS conjugates,

Proteinase K, Fluorescein/FITC labeled Cat. No. PK1-FL-1 2 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Proteinase K, Fluorescein/FITC labeled

Cat. No. PK1-FL-1
Specification
Unit Size 2 mg
Price $385.00

Qty Add to Cart

Proteinase K, Fluorescein Labeled

Synonym: Proteinase K FITC, FITC-Proteinase K, Proteinase K-FAM.

Similar products: Alexa Fluor 488 Proteinase K, SF488 Proteinase K.

Product Description:

Fluorescein labeled Proteinase K is one of Nanocs’ fluorescent Proteinase K conjugates. Proteinase K is a highly reactive nonspecific serine protease that is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9. Nanocs provides a number of fluorescent labeled Proteinase K products that span from UV region to near IR. These fluorescent Proteinase K conjugates can be used for enzyme studies, bioassay development and many other biomedical research. Nanocs Fluorescein labeled Proteinase K, FITC Proteinase K is a green fluorescent Proteinase K conjugate that has excitation/emission wavelength at 490/520 nm. This fluorescein Proteinase K can be easily detected by fluorescent instruments in FITC channel. All our fluorescent Proteinase K conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Product Specifications:

  • 2~5 fluorescein dyes on each Proteinase K molecule.
  • Molecular weight: ~28.5 kD
  • Proteinase K conjugates provided either in water solution or dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Dextran, FITC and Biotin labeled Cat. No. DX10-BNFC-1 10000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, FITC and Biotin labeled

Cat. No. DX10-BNFC-1
Specification 10000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

Biotin and FITC labeled Dextran is a multifunctional fluorescent dextran that has biotin groups. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 functional groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Dextran, FITC and Biotin labeled Cat. No. DX40-BNFC-1 40000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, FITC and Biotin labeled

Cat. No. DX40-BNFC-1
Specification 40000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

Biotin and FITC labeled Dextran is a multifunctional fluorescent dextran that has biotin groups. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 functional groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Dextran, FITC and Biotin labeled Cat. No. DX70-BNFC-1 70000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, FITC and Biotin labeled

Cat. No. DX70-BNFC-1
Specification 70000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

Biotin and FITC labeled Dextran is a multifunctional fluorescent dextran that has biotin groups. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 functional groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Dextran, FITC and Biotin labeled Cat. No. DX500-BNFC-1 500000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, FITC and Biotin labeled

Cat. No. DX500-BNFC-1
Specification 500000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

Biotin and FITC labeled Dextran is a multifunctional fluorescent dextran that has biotin groups. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 functional groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Fluorescein PEG, mPEG-FITC Cat. No. PG1-FC-5k 5000 Da 50 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Fluorescein PEG, mPEG-FITC

Cat. No. PG1-FC-5k Fluorescein PEG, mPEG-FITC           Cat. No. PG1-FC-5k     5000 Da    50 mg
Specification 5000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.

Physical Properties:

  • Organge/yellow solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Fluorescein PEG, mPEG-FITC Cat. No. PG1-FC-2k 2000 Da 50 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Fluorescein PEG, mPEG-FITC

Cat. No. PG1-FC-2k Fluorescein PEG, mPEG-FITC           Cat. No. PG1-FC-2k     2000 Da    50 mg
Specification 2000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.

Physical Properties:

  • Organge/yellow solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Fluorescein PEG, mPEG-FITC Cat. No. PG1-FC-1k 1000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Fluorescein PEG, mPEG-FITC

Cat. No. PG1-FC-1k Fluorescein PEG, mPEG-FITC           Cat. No. PG1-FC-1k     1000 Da    25 mg
Specification 1000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.

Physical Properties:

  • Organge/yellow solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Silica nanoparticles, FITC lableled Cat. No. Si10-FC-1 10 nm 1 mL修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Silica nanoparticles, FITC lableled

Cat. No. Si10-FC-1 Silica nanoparticles, FITC lableled           Cat. No. Si10-FC-1     10 nm    1 mL
Specification 10 nm
Unit Size 1 mL
Price $385.00

Qty Add to Cart

Silica Nanoparticles, fluorescein (FITC) Labeled:


Nanocs offers a number of Fluorescent silica nanoparticles and silica microbeads with fluorescent signal from UV region to near infrared area. Fluorescein labeled silica beads are green fluorescent particles with excitation/emission wavelength around 490 nm/515 nm. Silica particles from Nanocs have narrow size distribution yet with multiple size selection from 7 nm to 50 microns. These particles are suspended in 1% aqeuous solution. Standard deviation of these particles is in the range of 10~20% of listed size parameter. These particles have wide applications in diagnostic reagents and bioassay development. They can be further modified with different surface coatings and functional groups. Nanocs fluorescent labeled silica particles provide more choices for in-vitro or in-vivo applications of those nanoparticles. These particles include various fluorescent dyes from UV region to near IR, include coumarin, fluoresceins, rhodamines, cyanine dyes, alexa fluor dyes. Customer conjugation and modification is available upon request.


Silica nanoparticles, FITC lableled           Cat. No. Si10-FC-1     10 nm    1 mL

Bead Properties:

  • Concentration: 1% suspension in aqueous solution, other concentration available upon request;
  • Color: Green to yellow;
  • Excitation/Emission: ~495 nm/515 nm;

Storage Conditions:

  • Store at 4 0C. Protect from light. DO NOT FREEZE.

Tags: Fluorescent Silica Beads,

Fluorescein PEG, mPEG-FITC Cat. No. PG1-FC-10k 10000 Da 50 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Fluorescein PEG, mPEG-FITC

Cat. No. PG1-FC-10k Fluorescein PEG, mPEG-FITC           Cat. No. PG1-FC-10k     10000 Da    50 mg
Specification 10000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.

Physical Properties:

  • Organge/yellow solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Silica nanoparticles, FITC lableled Cat. No. Si15-FC-1 15 nm 1 mL修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Silica nanoparticles, FITC lableled

Cat. No. Si15-FC-1 Silica nanoparticles, FITC lableled           Cat. No. Si15-FC-1     15 nm    1 mL
Specification 15 nm
Unit Size 1 mL
Price $385.00

Qty Add to Cart

Silica Nanoparticles, fluorescein (FITC) Labeled:


Nanocs offers a number of Fluorescent silica nanoparticles and silica microbeads with fluorescent signal from UV region to near infrared area. Fluorescein labeled silica beads are green fluorescent particles with excitation/emission wavelength around 490 nm/515 nm. Silica particles from Nanocs have narrow size distribution yet with multiple size selection from 7 nm to 50 microns. These particles are suspended in 1% aqeuous solution. Standard deviation of these particles is in the range of 10~20% of listed size parameter. These particles have wide applications in diagnostic reagents and bioassay development. They can be further modified with different surface coatings and functional groups. Nanocs fluorescent labeled silica particles provide more choices for in-vitro or in-vivo applications of those nanoparticles. These particles include various fluorescent dyes from UV region to near IR, include coumarin, fluoresceins, rhodamines, cyanine dyes, alexa fluor dyes. Customer conjugation and modification is available upon request.


Silica nanoparticles, FITC lableled           Cat. No. Si15-FC-1     15 nm    1 mL

Bead Properties:

  • Concentration: 1% suspension in aqueous solution, other concentration available upon request;
  • Color: Green to yellow;
  • Excitation/Emission: ~495 nm/515 nm;

Storage Conditions:

  • Store at 4 0C. Protect from light. DO NOT FREEZE.

Tags: Fluorescent Silica Beads,

Fluorescein PEG, mPEG-FITC Cat. No. PG1-FC-20k 20000 Da 50 mg修饰性聚乙二醇

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Fluorescein PEG, mPEG-FITC

Cat. No. PG1-FC-20k Fluorescein PEG, mPEG-FITC           Cat. No. PG1-FC-20k     20000 Da    50 mg
Specification 20000 Da
Unit Size 50 mg
Price $385.00

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Description:

Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.

Physical Properties:

  • Organge/yellow solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet