EC 1.1.1.37 From E. coli. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogeneous (MW 34,500). In 3.2 M ammonium sulphate. Specific activity: 1130 U/mg (25°C, pH 7.5. on oxaloacetic acid. Contaminants:L-glutamic-oxaloacetic transaminase = 0.0058 %, NADH oxidase < 0.001 %. Stable at 4oC for > 4 years.
EC 1.1.1.27 (S)-lactate:NAD+ oxidoreductase. From porcine origin. In 3.2 M ammonium sulphate. Specific activity: ~ 330 U/mg (37oC, pH 7.0). Stability: > 2 years at 4oC
EC 3.2.1.73 CAZY Family: GH16 From B. subtilis. Electrophoretically homogeneous. In 3.2 M ammonium sulphate. Specific activity: 336 U/mg (40°C, pH 6.5, barley ?-glucan). 1000 U/ml. Contaminants: α-amylase < 0.0001%. Stable at 4°C for > 4 years.
EC 3.2.1.39 From Trichoderma sp. Highly purified. In 3.2 M ammonium sulphate. Specific Activity: ~ 31 U/mg (40°C, pH 4.5, CM-Curdlan as substrate). Stable at 4°C for > 4 years.
EC 3.2.1.39 From Trichoderma sp. Highly purified. In 3.2 M ammonium sulphate. Specific Activity: ~ 31 U/mg (40°C, pH 4.5, CM-Curdlan as substrate). Stable at 4°C for > 4 years
EC 3.2.1.39 CAZY Family: GH17 Recombinant. From barley (Hordeum vulgare). In 3.2 M ammonium sulphate. Specific activity: ~ 220 U/mg (40°C, pH 5.0 on laminarin) Store at 4°C.
EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6
Synonyms:
isoamylase; glycogen 6-alpha-D-glucanohydrolase
Form:
In 3.2 M ammonium sulphate.
Stability:
> 4 years at 4oC.
Specific activity:
~ 240 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).
Unit definition:
One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC.
EC 3.2.1.26 From yeast.
Chromatographically purified.
In 50% glycerol.
Specific activity: 900 U/mg protein (40°C, pH 4.5, 1 % sucrose). 2000 U/ml.
Contaminants: α- and β-galactosidase < 0.001%, α- and β-glucosidase < 0.001 %.
Stable at 4°C for > 4 years.
EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6
Synonyms:
isoamylase; glycogen 6-alpha-D-glucanohydrolase
Form:
In 3.2 M ammonium sulphate.
Stability:
> 4 years at 4oC.
Specific activity:
~ 180 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).
Unit definition:
One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC.
Specificity:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Applications:
Applications in carbohydrate research and in the food and feeds, and cereals industry.
EC 3.2.1.26 β-Fructofuranosidase.
Sucrase. Saccharase.
Specific activity: 300 U/mg of solid.
Contaminants: α-galactosidase, β-galactosidase, α-glucosidase and β-glucosidase less than 0.001 %.
EC 3.2.1.26 β-Fructofuranosidase.
Sucrase. Saccharase.
Specific activity: 300 U/mg of solid; 900 U/mg protein.
Contaminants: α-galactosidase, β-galactosidase, α-glucosidase and β-glucosidase less than 0.001 %.
EC 1.1.1.18 Inositol 2-dehydrogenase; myo-Inositol : NAD+ 2-oxidoreductase.
Recombinant from B. subtilis.
In 3.2 M ammonium sulphate.
Specific activity: ~ 50 U/mg (25oC, pH 9.6, myo-inositol).
Stable at 4oC for > 2 years.
EC 1.1.1.42 Recombinant from B. subtilis. In 3.2 M ammonium sulphate.
Specific activity: ~ 25 U/mg (25oC, pH 7.6).
Contaminants: NADH oxidase < 0.001 %, NADPH oxidase < 0.01 %.
Stable at 4oC for > 2 years.
EC 4.2.2.1 CAZY Family: PL8 Recombinant.
From a soil prokaryote.
In 3.2 M ammonium sulphate.
Specific activity: ~ 90 U/mg (25oC, pH 4.2 on hyaluronic acid).
Store at 4oC.
EC 2.7.1.1 and EC 1.1.1.49.
From yeast and Leuconostoc mesenteroides, respectively.
In 3.2 M ammonium sulphate.
Activity: Hexokinase > 420 U/mL (25°C, pH 7.4, glucose); glucose-6-phosphate dehydrogenase > 210 U/mL (25°C, pH 7.4, glucose-6-phosphate).
For use in determination of D-glucose.
Stable at 4°C for > 4 years.
D-Malate dehydrogenase (E. coli) EC 1.1.1.83 Recombinant from E.coli.
In 3.2 M ammonium sulphate.
Specific activity: ~20 U/mg (25°C pH 7.0).
Contaminants: NADH oxidase < 0.002%.
Stable between -10°C and -20°Cfor > 2 years.
EC 1.1.1.28 New and Improved.
Recombinant from L. mesenteroide.
In 3.2 M ammonium sulphate.
Specific activity: 887 U/mg (25oC, pH 7.0).
Contaminants: L-malate dehydrogenase = 0.04%;
GOT, GPT, myokinase, NADH oxidase, pyruvate kinase < 0.001%.
Stable at 4oC for > 2 years.
EC 3.4.13.21 Recombinant from E. coli.
In 3.2 M ammonium sulphate.
Specific activity: ~ 100 U/mg (25oC, pH 8.0).
Contaminants: NADH oxidase < 0.0001 %
Stable at 4oC for > 2 years.
EC 1.8.1.4 From E. coli. In 3.2 M ammonium sulphate.
Specific activity: 69 U/mg (25°C, pH 9.0).
Stable at 4°C for > 2 years.