标签归档：assay

葡萄糖氧化酶检测试剂盒 Glucose Oxidase Assay Kit 货号：K-GLOX Megazyme试剂盒

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葡萄糖氧化酶检测试剂盒

英文名：Glucose Oxidase Assay Kit

货号：K-GLOX

规格：200 assays (manual) / 2000 assays (microplate)

市场价： 2862元

The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.

Glucose Oxidase assay kit is suitable for manual, auto-analyser and microplate formats.
 

Colourimetric method for the determination of Glucose Oxidase
in foodstuffs and fermentation products

Principle:
(glucose oxidase)
(1) D-Glucose + H2O + O2 → D-glucono-δ-lactone + H2O2

(peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size:                            200 assays (manual) / 2000 (microplate)
                                         / 1960 (auto-analyser)
Method:                            Spectrophotometric at 510 nm
Reaction time:                   ~ 20 min
Detection limit:                 10 U/L
Application examples: 
Enzyme preparations, and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:          Novel method

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

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海藻糖检测试剂盒Trehalose Assay Kit Trehalose Assay Kit 货号：K-TREH Megazyme试剂盒

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海藻糖检测试剂盒Trehalose Assay Kit

英文名：Trehalose Assay Kit

货号：K-TREH

规格：100 assays (manual) / 1000 assays (microplate)

市场价： 4452元

The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Trehalose and
D-Glucose in foodstuffs, beverages, and other materials

Principle:
(trehalase)
(1) Trehalose + H2O → D-glucose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                            100 assays (manual) / 1000 (microplate) 
                                          / 1100 (auto-analyser)
Method:                             Spectrophotometric at 340 nm
Reaction time:                   ~ 8 min
Detection limit:                  37.5 mg/L
Application examples: 
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:           Novel method

Advantages

  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

 

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q5. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q6. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q8. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q9. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q10. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q11. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q12. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q13. Must the minimum absorbance change for a sample always be at least 0.1?

No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results. 

Q14. Can the sensitivity of the kit assay be increased?

Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.

L-苹果酸检测试剂盒 L-Malic Acid Assay Kit (Manual Format) 货号：K-LMAL-116A Megazyme试剂盒

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L-苹果酸检测试剂盒

英文名：L-Malic Acid Assay Kit (Manual Format)

货号：K-LMAL-116A

规格：116 assays per kit

市场价： 3604元

L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size:                        (K-LMALR) 
                                     58 assays (manual) / 580 (microplate) or 
                                     (K-LMALL) 
                                     116 assays (manual) / 1160 (microplate)
Method:                         Spectrophotometric at 340 nm
Reaction time:               ~ 3 min
Detection limit:              0.25 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by AOAC, EEC, 
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format

Q1. What is the difference between K-LMAL-58A / 116A, K-LMALAF, K-LMALMQ and K-LMALQR?

Megazyme produces 4 L-malic acid test kits:
K-LMAL-58A / 116A: UV method, automated format for use with auto-analysers.
K-LMALAF: UV method, manual format for use with spectrophotometers.
K-LMALMQ: Colourimetric method, manual format for use with hand held colorimeter.
K-LMALQR: UV method, liquid ready reagents automated format for use with auto-analysers.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Which L-Malic Acid Kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore, K-LMALAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-LMAL-58A / 116A may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.
Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.

L-Malic Acid Kit Recommendation For Microplate Format:
Either K-LMAL-58A / 116A or K-LMALAF is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-LMAL-58A / 116A:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3.
The main advantage here is that if this kit is used with a microplate reader that has a pathlength conversion capability, or if results are converted as outlined above in point 3, then this enables easy calculation of results using the K-LMAL-58A / 116A MegaCalc application (available on the Megazyme website where the product is located).
K-LMALAF:
This kit is designed for use in an auto-analyser and therefore can be used without any modification to assay volumes directly in a 96-well microplate format. 
This kit has less reagent additions than K-LMAL-58A / 116A.
K-LMALAF does not have a MegaCalc application available to enable easy results calculation which therefore must be achieved as outlined above in either of points 2 or 3.

Q6. Do samples require any specific sample preparation prior to testing with the kits?

The sample preparation is sample dependent, some samples may be tested directly in the assay or after appropriate dilution, however, some samples may require further sample preparation prior to testing.  The following are example of sample preparation methods:
(a) Liquid samples: clear, slightly coloured and approximately neutral, liquid samples can be used directly in the assay.
(b) Acidic samples: if > 0.1 mL of an acidic sample is to be used undiluted (such as wine or fruit juice), the pH of the solution should be increased to approx. 9.0 using 2 M NaOH, and the solution incubated at room temperature for 30 min.
(c) Carbon dioxide: samples containing significant quantities of carbon dioxide, such as beer, should be degassed by increasing the pH to approx. 9.0 with 2 M NaOH and gentle stirring, or by stirring with a glass rod.
(d) Coloured samples: an additional sample blank, i.e. sample with no L-MDH, may be necessary in the case of coloured samples.
(e) Strongly coloured samples: if used undiluted, strongly coloured samples should be treated by the addition of 0.2 g of PVPP/10 mL of sample.  Shake the tube vigorously for 5 min and then filter through Whatman No. 1 filter paper.
(f) Solid samples: homogenise or crush solid samples in distilled water and filter if necessary.
(g) Samples containing fat: extract such samples with hot water at a temperature above the melting point of the fat, e.g. in a 100 mL volumetric flask.  Adjust to room temperature and fill the volumetric flask to the mark with distilled water.  Store on ice or in a refrigerator for 15-30 min and then filter.  Discard the first few mL of filtrate, and use the clear supernatant (which may be slightly opalescent) for assay. Alternatively, clarify with Carrez reagents.
(h) Samples containing protein: deproteinise samples containing protein by adding an equal volume of ice-cold 1 M perchloric acid with mixing.  Centrifuge at 1,500 g for 10 min and neutralise the supernatant with 1 M KOH.  Alternatively use Carrez reagents.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

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Megazyme L-苹果酸（手动）检测试剂盒操作视频（K-LMAL）

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

L-苹果酸检测试剂盒 L-Malic Acid Assay Kit (Manual Format) 货号：K-LMAL-58A Megazyme试剂盒

发表于由
L-苹果酸检测试剂盒

英文名：L-Malic Acid Assay Kit (Manual Format)

货号：K-LMAL-58A

规格：58 assays per kit

市场价： 2000元

L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size:                        (K-LMALR) 
                                     58 assays (manual) / 580 (microplate) or 
                                     (K-LMALL) 
                                     116 assays (manual) / 1160 (microplate)
Method:                         Spectrophotometric at 340 nm
Reaction time:               ~ 3 min
Detection limit:              0.25 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by AOAC, EEC, 
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • PVP incorporated to prevent tannin inhibition
  • Both enzymes supplied as stable suspensions
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction (~ 3 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual and microplate format

Q1. What is the difference between K-LMAL-58A / 116A, K-LMALAF, K-LMALMQ and K-LMALQR?

Megazyme produces 4 L-malic acid test kits:
K-LMAL-58A / 116A: UV method, automated format for use with auto-analysers.
K-LMALAF: UV method, manual format for use with spectrophotometers.
K-LMALMQ: Colourimetric method, manual format for use with hand held colorimeter.
K-LMALQR: UV method, liquid ready reagents automated format for use with auto-analysers.

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q5. Which L-Malic Acid Kit is recommended for a 96-well microplate format?

Auto-analysers use ~ 0.315 mL reaction volumes and pathlengths between 4-8 mm which is similar to a standard 96-well microplate where a 0.315 mL reaction volume would give a pathlength of ~ 6-7 mm.  Therefore, K-LMALAF can be used directly in a 96-well microplate format with minimal assay optimisation.
If preferred, K-LMAL-58A / 116A may also be easily converted for use in a 96-well microplate format.  Basically, the assay volumes for the cuvette format must be reduced approximately 10-fold for use in a 96-well microplate.  However, some assay optimisation may be required (e.g. increased enzyme concentration etc.) and unlike the cuvette which has a set pathlength of 1 cm, the pathlength in the microplate is dependent upon the volume of liquid in the well.
Therefore to enable the calculation of the amount of analyte in the samples from tests performed in the microplate format one of the following must be done:

  1. The easiest method is to use a microplate reader that has a pathlength conversion capability (i.e. the microplate reader can detect the pathlength of each well and convert the individual readings to a 1 cm pathlength).  This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm pathlength) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values.

L-Malic Acid Kit Recommendation For Microplate Format:
Either K-LMAL-58A / 116A or K-LMALAF is recommended for use in a 96-well microplate format and the main advantages / disadvantages are described below:
K-LMAL-58A / 116A:
The assay volumes of this kit should be reduced by 10-fold for use in a 96-well microplate format (some assay optimisation may be required, e.g. increased enzyme concentration etc.).
The calculation of results is achieved as outlined above in either of points 1, 2 or 3.
The main advantage here is that if this kit is used with a microplate reader that has a pathlength conversion capability, or if results are converted as outlined above in point 3, then this enables easy calculation of results using the K-LMAL-58A / 116A MegaCalc application (available on the Megazyme website where the product is located).
K-LMALAF:
This kit is designed for use in an auto-analyser and therefore can be used without any modification to assay volumes directly in a 96-well microplate format. 
This kit has less reagent additions than K-LMAL-58A / 116A.
K-LMALAF does not have a MegaCalc application available to enable easy results calculation which therefore must be achieved as outlined above in either of points 2 or 3.

Q6. Do samples require any specific sample preparation prior to testing with the kits?

The sample preparation is sample dependent, some samples may be tested directly in the assay or after appropriate dilution, however, some samples may require further sample preparation prior to testing.  The following are example of sample preparation methods:
(a) Liquid samples: clear, slightly coloured and approximately neutral, liquid samples can be used directly in the assay.
(b) Acidic samples: if > 0.1 mL of an acidic sample is to be used undiluted (such as wine or fruit juice), the pH of the solution should be increased to approx. 9.0 using 2 M NaOH, and the solution incubated at room temperature for 30 min.
(c) Carbon dioxide: samples containing significant quantities of carbon dioxide, such as beer, should be degassed by increasing the pH to approx. 9.0 with 2 M NaOH and gentle stirring, or by stirring with a glass rod.
(d) Coloured samples: an additional sample blank, i.e. sample with no L-MDH, may be necessary in the case of coloured samples.
(e) Strongly coloured samples: if used undiluted, strongly coloured samples should be treated by the addition of 0.2 g of PVPP/10 mL of sample.  Shake the tube vigorously for 5 min and then filter through Whatman No. 1 filter paper.
(f) Solid samples: homogenise or crush solid samples in distilled water and filter if necessary.
(g) Samples containing fat: extract such samples with hot water at a temperature above the melting point of the fat, e.g. in a 100 mL volumetric flask.  Adjust to room temperature and fill the volumetric flask to the mark with distilled water.  Store on ice or in a refrigerator for 15-30 min and then filter.  Discard the first few mL of filtrate, and use the clear supernatant (which may be slightly opalescent) for assay. Alternatively, clarify with Carrez reagents.
(h) Samples containing protein: deproteinise samples containing protein by adding an equal volume of ice-cold 1 M perchloric acid with mixing.  Centrifuge at 1,500 g for 10 min and neutralise the supernatant with 1 M KOH.  Alternatively use Carrez reagents.

Q7. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q8. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

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Megazyme L-苹果酸（手动）检测试剂盒操作视频（K-LMAL）

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

Elabscience Cell Cycle Assay Kit代理商

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上海金畔生物科技有限公司是Elabscience Cell Cycle Assay Kit代理商 ，欢迎访问官网了解更多产品信息和订购。
Elabscience Cell Cycle Assay Kit代理

详情介绍

Elabscience Cell Cycle Assay Kit代理——上海金畔生物科技有限公司，Elabscience细胞周期检测试剂盒(绿色荧光)的荧光是Cycle Green，可选用FITC荧光通道进行检测。Elabscience细胞周期检测试剂盒保存条件是低温-20℃保存。

产品简介：

细胞周期（cell cycle）是指连续分裂细胞从一次有丝分裂结束到下一次有丝分裂结束所经历的整个过程。在这个过程中，细胞遗传物质复制并加倍，且在分裂结束时平均分配到两个子细胞中去。细胞周期又可以分为间期（interphase）和有丝分裂期(M phase)，细胞间期又常划分为休眠期（G0），DNA合成前期（G1），DNA合成期（S），DNA合成后期（G2）。DNA周期检测可用来反应细胞周期的各个期的状况，即细胞增殖状况。利用细胞内DNA能够和荧光染料（如Cycle Green）结合的特性，细胞各个时期其DNA含量不同从而结合的荧光染料不同，流式细胞仪检测的荧光强度也不一样可检测细胞不同周期。 细胞发生凋亡时，由于胞浆和染色质浓缩、核裂解，产生凋亡小体，使细胞的光散射性质发生变化。在细胞凋亡的早期，细胞前向光散射能力显著降低，侧向光散射能力增加或没有变化。在细胞凋亡的晚期，前向和侧向散射的信号均降低。因此可通过流式细胞仪测定细胞光散射的变化观察凋亡细胞。 Cycle Green染色后，假设G0/G1期细胞的荧光强度为1，那么含有双份基因组DNA的G2/M期细胞的荧光强度的理论值为2，正在进行DNA复制的S期细胞的荧光强度为1~2之间。凋亡细胞由于细胞核发生浓缩以及DNA片段化(DNA fragmentation)导致部分基因组DNA片段在染色过程中丢失，因此凋亡细胞Cycle Green染色后呈现明显的弱染，即荧光强度小于1，在流式检测的荧光图上出现所谓的sub-G1峰，即凋亡细胞峰。

产品属性：

应用类型：细胞周期

检测方法：荧光

样本类型；细胞

检测时间：3 hours

检测仪器：流式细胞仪

荧光：Cycle Green

Ex/Em (nm)：500/530

Channel set：FITC

自备试剂：无水乙醇，PBS(E-IR-R187)

保存温度：-20℃

运输条件：冰袋

保质期：12 months

产品订购：

货号

产品名称

规格

E-CK-A352

细胞周期检测试剂盒(绿色荧光)

20Assays

50Assays

100Assays


更多有关Elabscience Cell Cycle Assay Kit产品介绍，请联系Elabscience Cell Cycle Assay Kit代理——上海金畔生物科技有限公司：

Assay Biotech ELISA试剂盒代理商

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上海金畔生物科技有限公司是Assay Biotech ELISA试剂盒代理商 ，欢迎访问官网了解更多产品信息和订购。
Assay Biotech ELISA试剂盒代理

详情介绍

Assay Biotech ELISA试剂盒代理——上海金畔生物科技有限公司，提供Assay Biotech产品，如ELISA试剂盒、一抗、二抗等，更多Assay Biotech品牌产品等，欢迎咨询！

Assay Biotech公司介绍：

自 2006 年 1 月成立以来，Assay Biotechnology 一直是行业领xian的抗体和检测技术、荧光染料、淬灭剂、重组蛋白和合成肽的全球贡献者。我们位于加利福尼亚州旧金山湾区的中心地带，致力于满足全球对高质量检测试剂盒和免疫试剂的需求，这些检测试剂盒和免疫试剂用于学术和制药研究，为医疗保健奠定了基础。

Assay Biotech详细产品列表：

货号

产品

价格

品牌

CBP2128

IP3R-I (Phospho-Ser1764) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2127

Btk (Phospho-Tyr551) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2126

EpoR (Phospho-Tyr426) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2125

ZAP-70 (Phospho-Tyr319) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2124

YB-1 (Phospho-Ser102) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2123

XIAP (Phospho-Ser87) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2122

VDR (Phospho-Ser51) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2121

Vav3 (Phospho-Tyr173) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2120

TPH2 (Phospho-Ser19) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech

CBP2119

TGFβ RI (Phospho-Ser165) Colorimetric   Cell-Based ELISA Kit

询价

Assay Biotech


更多有关Assay Biotech产品介绍，请联系Assay Biotech ELISA试剂盒代理——上海金畔生物科技有限公司：

Enzo Biochem品牌代理商

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上海金畔生物科技有限公司是Enzo Biochem品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
Enzo Biochem

简要描述：

Enzo旗下共有Enzo，Biomol，Alexis Biochemicals，AssayDesigns，Stressgen等品牌，为生命科学研究者提供基因组分析，细胞生物学，翻译后修饰，信号转导，肿瘤和免疫学，药物筛选等相关领域的优质产品。

Enzo旗下共有Enzo，Biomol，Alexis Biochemicals，AssayDesigns，Stressgen等品牌，为生命科学研究者提供基因组分析，细胞生物学，翻译后修饰，信号转导，肿瘤和免疫学，药物筛选等相关领域的优质产品。Enzolife sciences 的标记技术和检测技术在科学研究和临床诊断这两个领域中处于地位。标记探针和染料的强强组合是基因表达分析，核酸检测，蛋白质生物化学研究与检测，分子生物学，细胞分析等研究领域中目标识别/验证和高容量分析实验的强有力工具。

Enzo Biochem是分子诊断的先驱，通过开发诊断平台技术，提供了优于以前标准的许多优势，从而临床实验室，生命科学和治疗学的融合。作为一家全球性公司，Enzo Biochem利用跨职能团队开发和部署产品系统和服务，以满足当今和未来医疗保健不断变化和快速增长的需求。支持Enzo Biochem的技术，平台和产品是一个广泛而深入的知识产权组合，覆盖了许多关键的支持技术。

Enzo Biochem is a leading life sciences and biotechnology company focused on harnessing genetic processes to develop research tools, diagnostics and therapeutics and provides reference laboratory services to the medical community. Founded in 1976, we have concentrated on the development of enabling technologies in the areas of gene regulation and gene modification. Many of our technologies are applicable to the biomedical and pharmaceutical research markets, and we are further using these technologies as a platform for our entry into the clinical diagnostics market. Today, Enzo technologies and products are recognized as the key tools in non-radioactive gene labeling used by researchers worldwide. Additionally, Enzo's work in gene analysis has led to development of a number of significant therapeutic candidates for the treatment of viral and immunological based disorders, several of which are in various phases of human clinical trials. In the course of our extensive research and development activities, we have built a significant patent position consisting of numerous pioneer patents and applications that encompass our core technologies.


The business activities of Enzo Biochem are performed by the company's three wholly owned subsidiaries . . . Enzo Life Sciences, Enzo Therapeutics and Enzo Clinical Labs. Such activities include research and development, manufacturing and marketing of biomedical research products and tools through Enzo Life Sciences and research and development of therapeutic products through Enzo Therapeutics and the operation of a regional clinical reference laboratory through Enzo Clinical Labs.


Enzo's vision of the importance of recombinant DNA technology as an informational source more than two decades ago has now become a major direction in modern medicine.

产品列表：



Enzo Biochem  BioArray(tm) HighYield(tm) RNA Transcript Labeling Kit (T7) 10 reactions 
Enzo Biochem  BioArray(tm) Eukaryotic Hybridization Controls 150 reactions 
Enzo Biochem  BioArray(tm) Deoxyoligo B2 150 reactions 
Enzo Biochem  Single Round Amplification and Labeling Systems  NA 
Enzo Biochem  Array CGH Labeling Kits 1 KT 
Enzo Biochem  Cyanine 5-NHS Ester Pack NA 
Enzo Biochem  Direct Labeling of cDNA NA 
Enzo Biochem  Cyanine-5-dUTP 25mmol
Enzo Biochem  Cyanine-5-UTP NA 
Enzo Biochem  Modified Nucleotides NA 
Enzo Biochem  Indirect Labeling Reactive Dye Packs NA 
Enzo Biochem  Ribonucleotides NA 
Enzo Biochem  Bio-17-ATP 250mmol'
Enzo Biochem  Fluorescein-12-UTP 250mmol'
Enzo Biochem  Bio-11-dCTP 50mmol

Dualucif® Firefly & Renilla Assay Kit（双萤光素酶报告基因检测试剂盒） 货号: F6075S/F6075M/F6075L 规格: 20T/100T/1000T

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

Dualucif® Firefly & Renilla Assay Kit（双萤光素酶报告基因检测试剂盒）

产品货号: F6075S/F6075M/F6075L

产品规格: 20T/100T/1000T

目录价（元）:286/1105/6189

推荐仪器：化学发光仪、酶标仪或液闪测定仪

大包装询价


产品概述：

储存条件

-80℃保存，有效期见外包装。C组分建议预先使用无菌水配置为2 mg/mL储液，B组分、D组分及配置为储液的C组分，根据实验需求进行小批量分装。所有检测工作液建议现配现用，避免反复冻融。

 

       规格

组分

F6075S（20T）

F6075M（100T）

F6075L（1000T）

A. 5× PassiveLuciferase Lysis Buffer

2 × 1 mL

10 mL

100 mL

B. FireflyLuciferase Assay Buffer

2 × 1 mL

10 mL

100 mL

C. D-Luciferin

0.4 mg

2 mg

20 mg

D. RenillaLuciferase Assay Buffer

2 × 1 mL

10 mL

100 mL

E. 50× Coelenterazine

40 µL

200 µL

2 × 1 mL

产品介绍

Dualucif® Firefly & Renilla Assay Kit（双萤光素酶报告基因检测试剂盒）为检测基因的表达量提供有效的手段，在 DLR检测中，萤火虫萤光素酶（Firefly luciferase）和海肾萤光素酶（Renilla luciferase）的活性可在单个样品中依次检测。先以萤光素（Luciferin）为底物来检测萤火虫萤光素酶的活性，然后加入抑制萤火虫萤光素酶催化的物质，同时加入腔肠素（Coelenterazine）检测海肾萤光素酶的活性，实现双萤光素酶报告基因检测。通过萤光素酶和其底物这一生物发光体系，可以非常灵敏、高效地检测基因的表达。通常把感兴趣基因的转录调控元件或5′ 启动子区克隆在Luciferase的上游，或把3′-UTR 区克隆在Luciferase的下游，构建成报告基因（Reporter gene）质粒，然后转染细胞，用适当药物等处理细胞后裂解细胞，通过检测萤光素酶活性的高低来判断药物处理等对目的基因的转录调控作用。海肾萤光素酶更多地被用作检测转染效率的内参，以消除细胞数量和转染效率的差异。

萤火虫萤光素酶是一种分子量约为61 kD 的蛋白，在ATP、镁离子和氧气存在的条件下，可以催化萤光素生成氧化萤光素（Oxyluciferin），在萤光素被氧化的过程中，会产生光信号。海肾萤光素酶是一种分子量约为36 kD的蛋白，在氧气存在的条件下，可以催化腔肠素氧化成肠酰胺（Coelenteramide），在腔肠素氧化的过程中也会产生光信号。本试剂盒的光信号可以通过化学发光仪、酶标仪或液闪测定仪进行测定。该试剂盒具有检测迅速、灵敏度高、检测范围广，无细胞内源活性干扰等特点。

注意事项

1. 使用前请将产品瞬时离心至管底，再进行后续实验。

2. 为取得最佳测定效果，在用单管的化学发光仪测定时，样品和测定试剂混合后到测定前的时间应尽量控制一致；使用具有化学发光测定功能的多功能荧光酶标仪时，宜先把样品全部加好，然后统一加入萤火虫萤光素酶检测试剂。

3. 萤火虫萤光素酶催化的生物发光的最强波长为560 nm，海肾萤光素酶催化的生物发光的最强波长为480 nm。

4. 为防止孔间干扰，建议使用白色不透光孔板。

5. 由于温度对酶反应有影响，所以测定时，样品和试剂均需达到室温后再进行测定。

6. 为了您的安全和健康，请穿实验服并戴一次性手套操作。


说明书：

Dualucif® Firefly & Renilla Assay Kit（双萤光素酶报告基因检测试剂盒） 货号: F6075S/F6075M/F6075L 规格: 20T/100T/1000T UE-F6075S/F6075M/F6075L    
MSDS：
MSDS F6075 Dual Luciferase Assay Kit
常见问题解答：

◆双萤光素酶报告基因最适反应温度？

室温（20-22℃）。反应时各个组分（细胞裂解产物，底物工作液等）都需要调整到室温。此两种萤光素酶的反应速率是受温度影响的，为了保证实验的一致性，我们推荐此两种工作液在检测时都孵育至室温。

◆双萤光素酶报告基因载体该如何选择？
（1）萤火虫报告基因质粒原则上含有luc基因就可以，但是不同实验所需的载体有一定差异，如果您要自己构建载体，要注意有些载体没有启动子，需要自行插入启动子，如pGL3 Basic。
（2）海肾报告基因质粒尽量选择中等强度的启动子，如TK启动子等，不要选择强启动子CMV、SV40等。海肾基因的表达活性应显著高于背景组，同时不干扰萤火虫萤光素酶报告基因的表达。。

◆检测的萤光数值很低怎么办？
（1）可能为转染效率低
a.优化转染实验条件，用较易转染的质粒做阳性对照（如转染过表达荧光蛋白质粒）；
b.确保转染DNA的质量，可通过酶切或琼脂糖凝胶电泳的方法对DNA质量进行鉴定；
c.选择活性较高，处于指数分裂期的细胞进行转染。
（2）可能为启动子活性低或诱导失败
a.转染后的细胞培养使用特异性诱导启动子的条件；
b.优化细胞的培养条件，提高萤光素酶的表达量；
c.更换强启动子（如SV40、CMV）。
注：海肾萤光素酶基因作为内对照，其表达应不受时期、部位、环境影响，因此常用组成型表达的TK启动子。
（3）样品裂解效率低
a.细胞培养时间不宜过长，12-36h内最好，长时间培养后，细胞可能会难裂解。
b.加入的裂解液需足量，保证细胞能够充分裂解。
（4）检测过程操作不规范
a.选择合适的检测仪器，能够检测化学发光或者生物发光的仪器都适用于该实验；
b.需加入足量底物，保证底物的饱和，否则会造成检测结果出现很大偏差；
c.室温反应。反应时各个组分（细胞裂解产物，底物工作液等）都需要调整到室温；
d.萤光素酶的半衰期一般约30min，加完底物后可立即检测，尽量在30min内完成。
（5）底物氧化失效
a.底物避光密封保存，萤火虫萤光素酶底物-20℃保存；海肾萤光素酶底物推荐-80℃保存；
b.反应工作液建议现用现配。


使用本产品的文献：

参考文献
1.DDX3 Activates CBC-eIF3-Mediated Translation of uORF-Containing Oncogenic mRNAs to Promote Metastasis in HNSCC.
应用方向：基因转录调控检测

2.Glucose starvation induces LKB1-AMPK-mediated MMP-9 expression in cancer cells.
应用方向：基因转录调控检测

3.Repression of MAP3K1 expression and JNK activity by canonical Wnt signaling.
应用方向：基因转录调控检测

4.The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.
应用方向：基因转录调控检测

5.Large-Scale Screening of Natural Products Transactivating Peroxisome Proliferator-Activated Receptor α Identifies 9S-Hydroxy-10E,12Z,15Z-Octadecatrienoic Acid and Cymarin as Potential Compounds Capable of Increasing Apolipoprotein A-I Transcription in Human Liver Cells.
应用方向：基因转录调控检测

BioPal品牌代理商

发表于由

上海金畔生物科技有限公司是BioPal品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
BioPal

简要描述：

BioPAL是生命科学领域高质量MRI对比试剂的供应商。我们提供广泛的铁基（T2成像剂，USPIOs）和钆基（T1成像剂）对比剂。 BioPAL还为其他成像系统提供和开发产品，如光学（近红外），核成像和CT。

BioPAL是生命科学领域高质量MRI对比试剂的供应商。我们提供广泛的铁基（T2成像剂，USPIOs）和钆基（T1成像剂）对比剂。 BioPAL还为其他成像系统提供和开发产品，如光学（近红外），核成像和CT。

 

BioPhysics Assay Laboratory (BioPAL, Inc.) is a privately held, for-profit company based in Worcester Massachusetts. The company developsand sells novel tools for the life science and biomedical communities. BioPAL was the first company to commercialize non-radioactive products thatuse neutron activation technology as the read-out system. The company also develops and sells nanoparticles for applications in magnetic resonance imaging (MRI), magnetic separation applications and tools for time resolved fluorescent technology. BioPAL offers ready-to-use cell labeling and tracking products for cellular therapy research applications.

 

BioPAL was the first to introduce functional immunoassay technology (FIT) to the research community. The company offers a line of renal diagnostics based on FIT to measure glomerular filtration rate (GFR) and effective renal blood flow (RBF). Most notably is BioPAL's ELISA kit for the measurement of inulin.

产品列表：

 

Magnetic Resonance Imaging (MRI) Agents

Optical, Near Infrared (NIR) Imaging Agents

Nuclear Imaging Agents (PET and SPECT)

Computed Tomography Imaging Agents (X-ray CT)

Glomerular Filtration Rate (FIT-GFR)

Effective Renal Blood Flow (FIT-RBF)

Stable-Labeled Microspheres – 15 µm

Stable-Labeled Microspheres – 10 µm

Contaminant-Free, BioPAL Sample Vials

Saline-Free Buffer

BioPAL's Microsphere Assay Service

Viahance™ – Small

Viahance™ – Large

Molday ION Dye Free™

Molday ION Rhodamine B™

Bioassay works品牌代理商

发表于由

上海金畔生物科技有限公司是Bioassay works品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
Bioassay works

简要描述：

BioAssay Works公司专注于以抗原和抗体为基础的检测技术，及其在横向层流免疫分析（lateral-flow immunoassay）中的应用。公司拥有众多受保护的专利性技术，特别是在生产*性、高灵敏度、浓缩金溶液和金结合物方面的专利技术。BioAssay Works公司还拥有其他C-FLAT@侧向横流测试（免疫层析试验）专利品台。

BioAssay Works公司专注于以抗原和抗体为基础的检测技术，及其在横向层流免疫分析（lateral-flow immunoassay）中的应用。公司拥有众多受保护的专利性技术，特别是在生产*性、高灵敏度、浓缩金溶液和金结合物方面的专利技术。BioAssay Works公司还拥有其他C-FLAT@侧向横流测试（免疫层析试验)专利品台。

BioAssay Works公司创始人为免疫检测及相关技术领域的者。公司已发展成为众多快速检测领域企业的合作伙伴。BioAssay Works开发的检测产品具有的品质，因此公司的研发科学家们也在业界享有良好的声誉。

BioAssay Works将具有ELISA方法的高灵敏度引入到快速检的免疫层析检测中，同时又保持了产品的操作简便性和耐用性。专利性的纳米技术、受专利保护的分析平台以及丰富的检测技术开发经验，使得BioAssary Works公司可满足广大IVD企业客户的需求和期望。

We focus on rapid, easy-to-use, lateral-flow immunoassays. We develop the crucial reagents, such as gold sols, gold conjugates, gold ribbon, etc. that are the backbone of any high-performance lateral-flow device. Superior reagents, coupled with our development and manufacturing expertise, yield superior assays.

BioAssay Works, LLC (BAW) powers the development of the finest rapid immunoassays. Utilizing our high sensitivity concentrated gold sols, high-performance gold conjugates, and our patented lateral-flow test platform, BAW develops and manufactures rugged lateral-flow tests that rival the sensitivity of ELISA. These tests can be read visually for quick positive/negative results or with simple instruments for more quantitative results.

BAW offers a growing line of products that enable researchers and assay developers to create their own rapid assays. In addition, we are the assay-development partner-of-choice for both early-stage and established companies. We have the capability to manufacture and package your rapid assay, regardless of the volume required.

产品列表：

Bioassay works

NG20-B001

Colloidal Gold Sol

1 mL

Bioassay works

NG20-B009

Colloidal Gold Sol

9 mL

Bioassay works

NG20-B044

Colloidal Gold Sol

44 mL

Bioassay works

NG20-B100

Colloidal Gold Sol

100 mL

Bioassay works

NG30-B001

Colloidal Gold Sol

1 mL

Bioassay works

NG30-B009

Colloidal Gold Sol

9 mL

Bioassay works

NG30-B044

Colloidal Gold Sol

44 mL

Bioassay works

NG30-B100

Colloidal Gold Sol

100 mL

Bioassay works

NG40-B001

Colloidal Gold Sol

1 mL

Bioassay works

NG40-B009

Colloidal Gold Sol

9 mL

Bioassay works

NG40-B044

Colloidal Gold Sol

44 mL

Bioassay works

NG40-B100

Colloidal Gold Sol

100 mL

Bioassay works

DGHG-B001

Goat anti-Human IgG (H+L)

1 mL

Bioassay works

DGHG-B009

Goat anti-Human IgG (H+L)

9 mL

Bioassay works

DGHA-B001

Goat anti-Human IgA (α Chain)

1 mL

Bioassay works

DGHA-B009

Goat anti-Human IgA (α Chain)

9 mL

Bioassay works

DGHM-B001

Goat anti-Human IgM (μ Chain)

1 mL

Bioassay works

DGHM-B009

Goat anti-Human IgM (μ Chain)

9 mL

Bioassay works

DGMG-B001

Goat anti-Mouse IgG (H+L)

1 mL

Bioassay works

DGMG-B009

Goat anti-Mouse IgG (H+L)

9 mL

Bioassay works

DGRG-B001

Goat anti-Rabbit IgG (H+L)

1 mL

Bioassay works

DGRG-B009

Goat anti-Rabbit IgG (H+L)

9 mL

Bioassay works

DRGG-B001

Rabbit anti-Goat IgG (H+L)

1 mL

Bioassay works

DRGG-B009

Rabbit anti-Goat IgG (H+L)

9 mL

Bioassay works

DPRA-B001

Protein A

1mL

Bioassay works

DPRA-B009

Protein A

9 mL

Bioassay works

DPRG-B001

Protein G

1 mL

Bioassay works

DPRG-B009

Protein G

9 mL

Bioassay works

DSTA-B001

Streptavidin

1 mL

Bioassay works

DSTA-B009

Streptavidin

9 mL

Bioassay works

DGBB-B001

Biotinylated BSA

1 mL

Bioassay works

DGBB-B009

Biotinylated BSA

9 mL

Bioassay works

DGMB-B001

Mouse anti-Biotin

1 mL

Bioassay works

DGMB-B009

Mouse anti-Biotin

9 mL

Firefly Luciferase Assay Kit（萤火虫萤光素酶报告基因检测试剂盒） 货号: F6024S/F6024M/F6024L/F6024XL 规格: 20T/50T/200T/1000T

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上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

Firefly Luciferase Assay Kit（萤火虫萤光素酶报告基因检测试剂盒）

产品货号: F6024S/F6024M/F6024L/F6024XL

产品规格: 20T/50T/200T/1000T

目录价（元）:264/516/1179/4274

推荐仪器：化学发光仪、酶标仪或液闪测定仪

大包装询价


产品概述：

储存条件

-80℃保存，有效期见外包装。C组分建议预先使用无菌水配置为2 mg/mL储液，B组分及配置为储液的C组分，根据实验需求进行小批量分装。检测工作液建议现配现用，避免反复冻融。

 

规格

组分

F6024S（20T）

F6024M（50T）

F6024L（200T）

F6024XL（1000T）

A. 5×FireflyLuciferase Lysis Buffer

2 × 1 mL

5 mL

20 mL

100 mL

B. FireflyLuciferase Assay Buffer

2 × 1 mL

5 mL

20 mL

100 mL

C. D-Luciferin

0.4 mg

1 mg

4 mg

20 mg

 

产品介绍

真核基因表达调控研究常用的方法是进行报告基因的检测，生物发光法又是报告基因检测最常用的有效手段。萤光素酶能催化底物萤光素的转化，并发射出光子。该产品为萤火虫萤光素酶报告基因在哺乳动物细胞中的表达提供快速、灵敏、稳定的检测方法。能够检测最低10-20 mol的萤光素酶，在10-14至10-20mol的酶浓度范围内呈很好的线性关系。

 

产品特点

1. 快速：细胞裂解在10-15 min内完成。

2. 方便：试剂易于配制，样品检测步骤简单。

3. 灵敏：能够检测最低10-20 mol的萤光素酶。

4. 酶的浓度线性范围可达8个数量级。

 

注意事项

1. 使用前请将产品瞬时离心至管底，再进行后续实验。

2. 温度会对酶的活性产生影响，因此所有试剂应放置室温后再使用。

3. 如果单管萤光测定仪测定，每个样品与测定试剂混合后到测定前的时间应保持一致。

4. 萤火虫萤光素酶催化的生物发光的最强波长为560 nm。

5. 为防止孔间干扰，建议使用白色不透光孔板。

6. 为了您的安全和健康，请穿实验服并戴一次性手套操作。


说明书：

Firefly Luciferase Assay Kit（萤火虫萤光素酶报告基因检测试剂盒） 货号: F6024S/F6024M/F6024L/F6024XL 规格: 20T/50T/200T/1000T UE-F6024S/F6024M/F6024L/F6024XL    
常见问题解答：

◆检测的萤光数值很低怎么办？

(1）可能为转染效率低
a.优化转染实验条件，用较易转染的质粒做阳性对照（如转染过表达荧光蛋白质粒）；
b.确保转染DNA的质量，可通过酶切或琼脂糖凝胶电泳的方法对DNA质量进行鉴定；
c.选择活性较高，处于指数分裂期的细胞进行转染。
(2）可能为启动子活性低或诱导失败
a.转染后的细胞培养使用特异性诱导启动子的条件；
b.优化细胞的培养条件，提高萤光素酶的表达量；
c.更换强启动子（如SV40、CMV）。
注：海肾萤光素酶基因作为内对照，其表达应不受时期、部位、环境影响，因此常用组成型表达的TK启动子。
(3）样品裂解效率低
a.细胞培养时间不宜过长，12-36h内最好，长时间培养后，细胞可能会难裂解。
b.加入的裂解液需足量，保证细胞能够充分裂解。
(4）检测过程操作不规范
a.选择合适的检测仪器，能够检测化学发光或者生物发光的仪器都适用于该实验；
b.需加入足量底物，保证底物的饱和，否则会造成检测结果出现很大偏差；
c.室温反应。反应时各个组分（细胞裂解产物，底物工作液等）都需要调整到室温；
d.萤光素酶的半衰期一般约30min，加完底物后可立即检测，尽量在30min内完成。
(5）底物氧化失效
a.底物避光密封保存，萤火虫荧光素酶底物-20℃保存；海肾萤光素酶底物推荐-80℃保存；
b.反应工作液建议现用现配。

◆进行检测的过程中，萤光信号值太高该如何进行调整？
（1） 减少质粒转染量。
（2）细胞样品裂解后，离心取上清后检测或对裂解产物进行稀释后检测。
注：不建议通过减少底物量来降低荧光值，需要保证底物的饱和来反映萤光素酶真实的表达水平，否则会造成检测结果出现大的偏差。

Cell Cycle Assay Kit Plus（细胞周期检测试剂盒升级版） 货号: C6078 规格: 50T

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

Cell Cycle Assay Kit Plus（细胞周期检测试剂盒升级版）

产品货号: C6078

产品规格: 50T

目录价（元）:600

推荐仪器：流式细胞仪

大包装询价


产品概述：

产品内容：

组分

50T

A. 染色缓冲液（10×）

10 mL

B. RedNucleus I 染色液

200 μL


储存条件
4℃保存，有效期见外包装。长期储存请于-20℃保存。RedNucleus I染色液需避光保存。

产品介绍

Cell Cycle Assay Kit Plus（细胞周期检测试剂盒升级版）用于活细胞及固定细胞周期检测具有一定的适用性，目前经过本公司验证过的细胞类型有Hela、Molt-4、Jurkat、K562，对于Hela及Molt-4，活细胞及75%冰乙醇-20℃过夜固定后均可以检测，但另外两种Jurkat、K562，活细胞状态及固定状态均不能检测。故针对不同类型的细胞，是否适用，需要测试后确定。
Cell Cycle Assay Kit Plus（细胞周期检测试剂盒升级版）采用RedNucleus I染色法检测细胞周期，RedNucleus I是一种远红外核酸染料，具有细胞膜透性，可快速进入活细胞，特异结合DNA，对活细胞进行周期检测，且无需RNase的消化。与传统的碘化丙啶染色（PI staining）方法相比，细胞无需破膜或固定，操作更加简便。
RedNucleus I是一种双链DNA的荧光染料，与双链DNA结合后的荧光强度和双链DNA的含量成正比。可通过流式细胞仪测定细胞内DNA含量，然后根据DNA含量的分布情况，进行细胞周期分析。RedNucleus I 染色后，假设G0/G1期细胞的荧光强度为1，那么含有双份基因组DNA的G2/M期细胞的荧光强度的理论值为2，正在进行DNA复制的S期细胞的荧光强度为1-2之间。此外，RedNucleus I与Horizon BV/BUV、FITC和R-PE等染料兼容，可在样品染色后进行周期检测。
本试剂盒通常应用于培养的贴壁或悬浮细胞的细胞周期检测，如果用于组织的细胞周期检测，则必须先把组织消化成单细胞状态。

注意事项

1. 使用前请将产品瞬时离心至管底，再进行后续实验。

2. 本产品适用于活细胞和固定细胞周期检测具有一定的局限性，针对不同类型的细胞，是否适用，需要测试后确定。如需固定，推荐使用冰浴预冷75-80%的乙醇-20℃过夜固定细胞。

3. 荧光染料均存在淬灭问题，保存和使用过程中请尽量注意避光，以减缓荧光淬灭。

4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。


说明书：

Cell Cycle Assay Kit Plus（细胞周期检测试剂盒升级版） 货号: C6078 规格: 50T UE-C6078    
MSDS：
MSDS C6078 Cell Cycle Assay Kit Plus

Renilla Luciferase Assay Kit（海肾萤光素酶报告基因检测试剂盒） 货号: R6073S/R6073M/R6073L 规格: 50T/200T/1000T

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

Renilla Luciferase Assay Kit（海肾萤光素酶报告基因检测试剂盒）

产品货号: R6073S/R6073M/R6073L

产品规格: 50T/200T/1000T

目录价（元）:663/1842/5526

推荐仪器：化学发光仪、酶标仪或液闪测定仪

大包装询价


产品概述：

储存条件

-80℃保存，有效期见外包装。B组分建议根据实验需求进行小批量分装。海肾检测工作液建议现配现用，避免反复冻融。


    规格

组分

R6073S（50T）

R6073M（200T）

R6073L（1000T）

A. 5× RenillaLuciferase   Lysis Buffer

5 mL

20 mL

100 mL

B. RenillaLuciferase   Assay Buffer

5 mL

20 mL

100 mL

C. 50× Coelenterazine

100 µL

400 µL

2 × 1 mL

 

产品介绍

真核基因表达调控研究常用的方法是进行报告基因的检测，生物发光法又是报告基因检测最常用的有效手段。萤光素酶能催化底物萤光素的转化，并发射出光子。该产品为海肾萤光素酶报告基因在哺乳动物细胞中的表达提供快速、灵敏、稳定的检测方法。

 

产品特点

1. 快速：细胞裂解在10-15 min内完成。

2. 方便：试剂易于配制，样品检测步骤简单。

 

注意事项

1. 使用前请将产品瞬时离心至管底，再进行后续实验。

2. 由于温度对酶反应有影响，所以测定时，样品和试剂均需达到室温后再进行测定。

3. 海肾萤光素酶催化的生物发光的最强波长为480 nm，为防止孔间干扰，建议使用白色不透光孔板。

4 为了您的安全和健康，请穿实验服并戴一次性手套操作。


说明书：

Renilla Luciferase Assay Kit（海肾萤光素酶报告基因检测试剂盒） 货号: R6073S/R6073M/R6073L 规格: 50T/200T/1000T UE-R6073S/R6073M/R6073L    
常见问题解答：

◆检测的萤光数值很低怎么办？

（1）可能为转染效率低
a.优化转染实验条件，用较易转染的质粒做阳性对照（如转染过表达荧光蛋白质粒）；
b.确保转染DNA的质量，可通过酶切或琼脂糖凝胶电泳的方法对DNA质量进行鉴定；
c.选择活性较高，处于指数分裂期的细胞进行转染。
（2）可能为启动子活性低或诱导失败
a.转染后的细胞培养使用特异性诱导启动子的条件；
b.优化细胞的培养条件，提高萤光素酶的表达量；
c.更换强启动子（如SV40、CMV）。
注：海肾萤光素酶基因作为内对照，其表达应不受时期、部位、环境影响，因此常用组成型表达的TK启动子。
（3）样品裂解效率低
a.细胞培养时间不宜过长，12-36h内最好，长时间培养后，细胞可能会难裂解。
b.加入的裂解液需足量，保证细胞能够充分裂解。
（4）检测过程操作不规范
a.选择合适的检测仪器，能够检测化学发光或者生物发光的仪器都适用于该实验；
b.需加入足量底物，保证底物的饱和，否则会造成检测结果出现很大偏差；
c.室温反应。反应时各个组分（细胞裂解产物，底物工作液等）都需要调整到室温；
d.萤光素酶的半衰期一般约30min，加完底物后可立即检测，尽量在30min内完成。
（5）底物氧化失效
a.底物避光密封保存，萤火虫萤光素酶底物-20℃保存；海肾萤光素酶底物推荐-80℃保存；
b.反应工作液建议现用现配。

◆进行检测的过程中，萤光信号值太高该如何进行调整？
（1） 减少质粒转染量。
（2）细胞样品裂解后，离心取上清后检测或对裂解产物进行稀释后检测。
注：不建议通过减少底物量来降低荧光值，需要保证底物的饱和来反映萤光素酶真实的表达水平，否则会造成检测结果出现大的偏差。

NO检测试剂盒（Nitric Oxide Assay Kit） 货号: N6025S/N6025L 规格: 200T/1000T

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

NO检测试剂盒（Nitric Oxide Assay Kit）

产品货号: N6025S/N6025L

产品规格: 200T/1000T

目录价（元）:239/799

推荐仪器：酶标仪

大包装询价


产品概述：
储存条件
-20℃避光保存，有效期见外包装。

组分

规格

N6025S（200T）

N6025L（1000T）

A. Griess Reagent I

10 mL

50 mL

B. Griess Reagent II

10 mL

50 mL

C. 1 M NaNO2

200 μL

1 mL

产品介绍
Griess 试剂可用于光度法检测亚硝酸盐。试剂含有两种化学物质，磺胺酸和 N-（1-萘基）乙二胺。在酸性条件下，磺胺酸被亚硝酸盐转化成重氮盐，可与 N-（1-萘基）乙二 胺形成高度着色的偶氮染料，可以在 548 nm 检测此染料：由于 NO 极其不稳定，被氧化生成亚硝酸盐和硝酸盐，Griess 通过检测亚硝酸盐的含量，间接反映出 NO 的含量。

注意事项
1. 在使用 Griess 试剂之前，将其恢复至室温，并检查溶液是否有沉淀。如果 Griess Reagent I 取出时含有沉淀，可将其置于 37℃水浴锅中，水浴直至沉淀溶解。
2. 本品有潜在危害性，避免长时间或反复接触。避免进入眼睛，皮肤或衣服上。请穿实验服并戴一次性手套操作。
说明书：

NO检测试剂盒（Nitric Oxide Assay Kit） 货号: N6025S/N6025L 规格: 200T/1000T UE-N6025S/N6025L    

One-Step Firefly Luciferase Assay Kit（一步法萤火虫萤光素酶报告基因检测试剂盒） 货号: F6072S/F6072M/F6072L 规格: 50T/500T/1000T

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

One-Step Firefly Luciferase Assay Kit（一步法萤火虫萤光素酶报告基因检测试剂盒）

产品货号: F6072S/F6072M/F6072L

产品规格: 50T/500T/1000T

目录价（元）:610/4863/6484

推荐仪器：化学发光仪、酶标仪或液闪测定仪

大包装询价


产品概述：

储存条件

-80℃保存，有效期见外包装。B组分建议预先使用无菌水配置为2 mg/mL储液，A组分及配置为储液的B组分，根据实验需求进行小批量分装。检测工作液建议现配现用，避免反复冻融。

                  规格

组分

F6072S（50T）

F6072M（500T）

F6072L（1000T）

A.      One-Step FireflyLuciferase Assay Buffer

5 mL

50 mL

100 mL

B.       D-Luciferin

1 mg

10 mg

20 mg

 

产品介绍

报告基因检测是现代分子生物学研究领域中分析结构基因旁侧区域潜在的顺式元件（如启动子、增强子和沉默子等）和反式作用因子相互作用关系的一种重要工具。

萤火虫萤光素酶被广泛应用于基因调控和药物筛选等方面。萤火虫萤光素酶是一种分子量约为61 kD的蛋白，在ATP、镁离子和氧气存在的条件下，可以催化萤光素生成氧化萤光素（Oxyluciferin），在萤光素被氧化的过程中，会产生光信号，如图1。

本试剂盒的光信号是一种瞬时光，在加入工作液后，需立即检测。光信号半衰期约为5 min。

One-Step Firefly Luciferase Assay Kit（一步法萤火虫萤光素酶报告基因检测试剂盒） 货号: F6072S/F6072M/F6072L 规格: 50T/500T/1000T

One-Step Firefly Luciferase Assay Kit（一步法萤火虫萤光素酶报告基因检测试剂盒） 货号: F6072S/F6072M/F6072L 规格: 50T/500T/1000T
图1. 萤火虫萤光素酶催化的化学发光反应

注意事项
1. 使用前请将产品瞬时离心至管底，再进行后续实验。
2. 萤火虫萤光素酶催化的生物发光的最强波长为560 nm。
3. 为防止孔间干扰，建议使用白色不透光孔板。


说明书：

One-Step Firefly Luciferase Assay Kit（一步法萤火虫萤光素酶报告基因检测试剂盒） 货号: F6072S/F6072M/F6072L 规格: 50T/500T/1000T UE-F6072S/F6072M/F6072L    
使用本产品的文献：

   

Qbtest®1× dsDNA HS Assay Kit（即用型预混液） 货号: Q2061S/Q2061L 规格: 100T/500T

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

Qbtest®1× dsDNA HS Assay Kit（即用型预混液）

产品货号: Q2061S/Q2061L

产品规格: 100T/500T

目录价（元）:850/2780

大包装询价


产品概述：

储存条件

4℃避光保存，有效期见外包装。

                         规格   

组分

Q2061S（100T）

Q2061L（500T）

浓度

储存

A. Qbtest® 1× dsDNA HS预混液

50 mL

250 mL

1×

2-6℃避光

B. Qbtest® 1× dsDNA标准液1

1 mL

5 mL

0 ng/μL 

2-6℃

C. Qbtest® 1× dsDNA标准液2

1 mL

5 mL

10 ng/μL

2-6℃

 

产品参数

Ex/Em: 480/520 nm（结合dsDNA）

 

产品介绍

该产品提供已经预混好的检测液，与Qbtest® X-Green II双链DNA定量试剂盒升级款（Q2038）相比，为客户节省了检测液的预混时间，使用更加方便。

Qbtest® 1× dsDNA HS Assay Kit是荧光检测dsDNA并进行定量的一种产品，这种检测方法非常快速、灵敏和精确，适用于NGS大规模DNA样品如cDNA文库的定量，并且在一定程度上耐受常见的污染物如盐类、游离核苷酸、去污剂和蛋白质。

Qbtest® 1× dsDNA HS Assay Kit包含即用型工作液（已预混荧光染料）和dsDNA标准品，检测时只需将样品（1 – 20 μL）和工作液混合，即可使用Qubit荧光仪进行读数，操作简便、数据可靠。Qbtest® 1× dsDNA HS Assay Kit（即用型预混液）检测浓度范围10 pg/μL – 100 ng/μL、检测质量范围0.2 – 100 ng，且线性关系较好(R2>0.99)。

该试剂盒可用于Qubit仪器，效能等同于Qubit™ 1× dsDNA HS Assay Kits。

注意事项

1. 使用前请将产品瞬时离心至管底，再进行后续实验。

2. 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

3. 请将试剂盒中的各组分平衡至室温后再进行定量检测。

4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。


说明书：

Qbtest®1× dsDNA HS Assay Kit（即用型预混液） 货号: Q2061S/Q2061L 规格: 100T/500T UE-Q2061S/Q2061L