EC 3.2.1.20 CAZY Family: GH31 From A. niger. In 3.2 M ammonium sulphate. Specific activity: 70 U/mg (40°C, pH 4.5, maltose as substrate). 2.5 U/mg (phenyl α-glucoside as substrate). Contaminants: α-amylase < 0.001%; amyloglucosidase < 0.01%. Stable at 4°C for > 4 years.
英文名：Amyloglucosidase Assay Reagent
货号：MR-AMGR3
规格：200 assays per kit
市场价： 5400元
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EC 3.2.1.51 CAZY Family: GH29 α-L-fucoside fucohydrolase. Recombinant from T. maritima. In 3.2 M ammonium sulphate. Specific activity: ~ 2.5 U/mg (25oC, pH 5.0); ~ 4 U/mg (37oC), ~ 15 U/mg (60oC), ~ 195 U/mg (95oC). Contaminants: p-NP-N-acetyl-β-D-glucosaminide, p-NP-α-D-galactoside, p-NP-β-D-galactoside, p-NP-β-D-glucoside, p-NP-α-D-mannoside, p-NP-β-D-mannoside, p-NP-β-D-xyloside < 0.001 %.
英文名：Beta-Glucosidase
货号：E-BGOSTM
规格：460 Units
市场价： 3700元
High purity beta-Glucosidase (Thermostable) (T. maritima) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.
EC 3.2.1.21
CAZy Family: GH1
From T. maritima.
In 3.2 M ammonium sulphate. Electrophoretically homogeneous (before BSA addition).
Specific activity: ~ 70 U/mg (40oC, pH 6.5, p-nitrophenyl β-glucoside).
Stable at 4oC for > 4 years.
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英文名：1，2-a-L-Fucosidase (microbial)
货号：E-FUCM
规格：3 Units
市场价： 3600元
High purity 1,2-alpha-L-Fucosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.63
CAZy Family: GH95
2-alpha-L-fucopyranosyl-beta-D-galactoside fucohydrolase
Recombinant from microbial source. In solution (Tris.HCl/NaCl/EDTA).
Hydrolysis of terminal non-reducing α-1,2-linked L-fucose residues from glycoproteins and oligosaccharides.
Specific activity: ~ 160 U/mg (37oC, pH 6.5 on 2’-fucosyllactose)
Stability: > 3 years at 4oC.
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EC 3.2.1.3 CAZY Family: GH15 From Rhizopus sp.
Ultra high purity.
Electrophoretically homogeneous. Freeze-dried powder.
Specific activity: approx. 35 U/mg (40°C, pH 4.5, soluble starch as substrate).
Contaminants: α-amylase < 0.0001%.
Store dry at -20°C. Stable for > 4 years at -20°C.
英文名：α-Rhamnosidase (prokaryote)
货号：E-RHAMS
规格：3000 Units at 50°C
市场价： 2968元
High purity recombinant alpha-Rhamnosidase (prokaryote) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.
EC 3.2.1.40
CAZy family: GH78
α-L-rhamnoside rhamnohydrolase.
Recombinant from a prokaryote.
In 3.2 M ammonium sulphate.
Specific activity: ~ 190 U/mg (50oC, pH 6.5 on p-nitrophenyl-α-L-rhamnoside)
Store > 2 years 4oC.
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EC 3.2.1.55 CAZy family: GH51 Recombinant. From Clostridium thermocellum In 3.2 M ammonium sulphate. Specific activity:: 58 U/mg protein on p-nitrophenyl-α-L-arabinofuranoside at pH 5.5 and 40oC (155 U/mg at pH 5.5 and 60oC. Stable at 4oC for ~ 4 years.
英文名：α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
货号：E-TSAGS
规格：3000 Units at 40°C /6000 Units at 60°C
市场价： 2968元
Alpha葡[萄]糖苷酶[嗜热脂肪芽孢杆菌]
EC 3.2.1.20
CAZY Family: GH13
Recombinant from Bacillus stearothermophilus. In 3.2 M ammonium sulphate.
Specific activity: ~ 280 U/mg (60oC, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside); ~ 135 U/mg (40oC, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside).
Action on other substrates: Blocked p-nitrophenol maltoheptaoside < 0.0001 %
Stability: > 4 years at 4oC.
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英文名：α-L-Arabinofuranosidase (Aspergillus niger)
货号：E-AFASE
规格：480 Units
市场价： 2968元
High purity alpha-L-Arabinofuranosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.55
CAZy Family: GH51
From A. niger.
In 3.2 M ammonium sulphate.
Specific activity: ~ 32 U/mg (40oC, pH 4.0, p-nitrophenyl α-L-arabinofuranoside);
~ 12.6 U/mg on sugar-beet arabinan;
~ 1.2 U/mg on wheat arabinoxylan.
Stable at 4oC for > 4 years.
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英文名：α-Galactosidase (Aspergillus niger)
货号：E-AGLAN
规格：2000 Units
市场价： 3100元
High purity alpha-Galactosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.22
CAZy Family: GH36
From A. niger. Highly purified.
In 3.2 M ammonium sulphate.
Specific activity: > 600 U/mg (40oC, pH 4.5, p-nitrophenyl α-D-galactopyranoside.
Stable at 4oC for > 4 years.
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英文名：α-Galactosidase (Guar)
货号：E-AGLGU
规格：1000 Units
市场价： 3100元
High purity alpha-Galactosidase (Guar) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.22
CAZy Family: GH27
From guar seed. Highly purified. Stabilised with BSA.
In 3.2 M ammonium sulphate.
Specific activity: 50 U/mg (40oC, pH 4.5, p-nitrophenyl α-D-galactopyranoside, before addition of BSA).
Stable at 4oC for > 4 years.
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英文名：Amyloglucosidase (A. niger)
货号：E-AMGDF-40ML
规格：140000
市场价： 2968元
High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.3
CAZy Family: GH15
Amyloglucosidase.
From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Electrophoretically homogeneous. Stabilised liquid in 50% glycerol.
Specific activity: approx. 36 U/mg (40oC, pH 4.5, soluble starch as substrate). 200 U/ml on p-nitrophenyl β-maltoside; 3,260 U/ml on soluble starch.
Stable at 4oC for > 4 years.
Data booklets for each pack size are located in the Technical Resources tab.
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英文名：Amyloglucosidase (Aspergillus niger) Powder
货号：E-AMGDFPD
规格：4 g – 36000 Units/g
市场价： 3498元
High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.3
CAZY Family: GH15
Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase (Cat. No. E-BLAAM) and protease powder (Cat. No. E-BSPRTPD). For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.
Specific activity: approx. 36 U/mg (40°C, pH 4.5, soluble starch as substrate). 3,100 U/g on p-nitrophenyl ß-maltoside; 36,000 U/g on soluble starch.
Stable at -20°C for > 4 years.
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英文名：α-D-Mannosidase (Bacteroides thetaiotaomicron)
货号：E-AMANBT
规格：10 Units
市场价： 2800元
High purity α-D-Mannosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.24
CAZy Family: GH92
CAS: 9025-42-7
alpha-mannosidase; alpha-D-mannoside mannohydrolase
Recombinant. From Bacteroides thetaiotaomicron.
In 3.2 M ammonium sulphate.
Supplied at ~ 100 U/mL.
Specific activity: ~ 8 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.5 and 40oC.
Stability: > 4 years at 4oC.
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英文名：Oligo-α-1,6-Glucosidase plus β-Galactosidase
货号：E-BGOG
规格：10,000 Units Oligo-α-1,6-Glucosidase plus 10,000 Units β-Galactosidase
市场价： 3074元 Megazyme 低聚-α-1,6-葡萄糖苷酶加β-半乳糖苷酶 Oligo-α-1,6-Glucosidase plus β-Galactosidase
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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
1 set of this bundle includes 2,000,000 units of O-Glycosidase and 2,000 units of Neuraminidase.
O-Glycosidase, also known as Endo-α-N-Acetylgalactosaminidase, catalyzes the removal of
Core 1 and Core 3 O-linked disaccharides from glycoproteins.
Neuraminidase is the common name for Acetyl-neuraminyl hydrolase ( Sialidase ). This Neura-minidase catalyzes the hydrolysis of α2-3, α2-6, α2-8 linked N-acetyl-neuraminic acid residues
from glycoproteins and oligosaccharides.
O-Glycosidase is cloned from Enterococcus faecalis and expressed in E.coli (1). Neuraminidase
is cloned from Clostridium perfringens (2) and overexpressed in E. coli (3).
The following reagents are supplied with this product:
Unit Definition
One unit of O-Glycosidase is defined as the amount of enzyme required to remove 0.68 nmol of O-linked disaccharide from 5 mg of Neuraminidase digested, non-denatured fetuin in 1 hour at 37°C in a total reaction volume of 100 µl (1 unit of both O-Glycosidase and PNGase F will remove equivalent molar amounts of O-linked disaccharides and N-linked oligosaccharides, respectively).
Non-denaturing Unit Definition of O-Glycosidase:
Two fold serial dilutions of O-Glycosidase are added to a reaction mixture of 5 mg of Neura-minidase digested fetuin with 1 X GlycoBuffer 2. The reaction is then incubated at 37°C for 1 hour. O-linked disaccharide carbohydrates are determined by Morgan and Elson Assay (4). Note: Under denaturing conditions the enzyme activity is increased two-fold. This observation is substrate dependent.
Unit Definition of Neuraminidase:
One unit of Neuraminidase is defined as the amount of enzyme required to cleave > 95% of the terminal α-Neu5Ac from 1 nmol Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl- coumarin (AMC), in 5 minutes at 37°C in a total reaction volume of 10 µl.
1X Glycoprotein Denaturing Buffer
0.5% SDS
40 mM DTT
1X NP-40
1% NP-40 in MilliQ-H2O
1X GlycoBuffer 2
Incubate at 37°C
1X GlycoBuffer 2:
50 mM sodium phosphate
pH 7.5 @ 25°C
Companion Products
α2-3,6,8 Neuraminidase
O-Glycosidase
PNGase F
PNGase F (Glycerol-free)
Endo H
Endo Hf
1.Since O-Glycosidase is inhibited by SDS, it is essential to have NP-40 in the reaction mixture. It is not known why this non-ionic detergent counteracts the SDS inhibition at the present time. Double digest with Endo H must have NP-40 present (NP-40 does not inhibit Endo H).
2.To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
1.Koutsioulis, D., Landry, D. and Guthrie, E.P. (2008). Glycobiology. 18, 799-805.
2.Roggentin, P. et al. (1988). FEBS Lett. 238, 31-34.
3.Guan, C. unpublished observations. New England Biolabs.
4.Morgan, W.T.J. and Elson, L.A. (1934). Biochem. J. 28, 988-995.
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
1 set of this bundle includes 2,000,000 units of O-Glycosidase and 2,000 units of Neuraminidase.
O-Glycosidase, also known as Endo-α-N-Acetylgalactosaminidase, catalyzes the removal of
Core 1 and Core 3 O-linked disaccharides from glycoproteins.
Neuraminidase is the common name for Acetyl-neuraminyl hydrolase ( Sialidase ). This Neura-minidase catalyzes the hydrolysis of α2-3, α2-6, α2-8 linked N-acetyl-neuraminic acid residues
from glycoproteins and oligosaccharides.
O-Glycosidase is cloned from Enterococcus faecalis and expressed in E.coli (1). Neuraminidase
is cloned from Clostridium perfringens (2) and overexpressed in E. coli (3).
The following reagents are supplied with this product:
Unit Definition
One unit of O-Glycosidase is defined as the amount of enzyme required to remove 0.68 nmol of O-linked disaccharide from 5 mg of Neuraminidase digested, non-denatured fetuin in 1 hour at 37°C in a total reaction volume of 100 µl (1 unit of both O-Glycosidase and PNGase F will remove equivalent molar amounts of O-linked disaccharides and N-linked oligosaccharides, respectively).
Non-denaturing Unit Definition of O-Glycosidase:
Two fold serial dilutions of O-Glycosidase are added to a reaction mixture of 5 mg of Neura-minidase digested fetuin with 1 X GlycoBuffer 2. The reaction is then incubated at 37°C for 1 hour. O-linked disaccharide carbohydrates are determined by Morgan and Elson Assay (4). Note: Under denaturing conditions the enzyme activity is increased two-fold. This observation is substrate dependent.
Unit Definition of Neuraminidase:
One unit of Neuraminidase is defined as the amount of enzyme required to cleave > 95% of the terminal α-Neu5Ac from 1 nmol Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl- coumarin (AMC), in 5 minutes at 37°C in a total reaction volume of 10 µl.
1X Glycoprotein Denaturing Buffer
0.5% SDS
40 mM DTT
1X NP-40
1% NP-40 in MilliQ-H2O
1X GlycoBuffer 2
Incubate at 37°C
1X GlycoBuffer 2:
50 mM sodium phosphate
pH 7.5 @ 25°C
Companion Products
α2-3,6,8 Neuraminidase
O-Glycosidase
PNGase F
PNGase F (Glycerol-free)
Endo H
Endo Hf
1.Since O-Glycosidase is inhibited by SDS, it is essential to have NP-40 in the reaction mixture. It is not known why this non-ionic detergent counteracts the SDS inhibition at the present time. Double digest with Endo H must have NP-40 present (NP-40 does not inhibit Endo H).
2.To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
1.Koutsioulis, D., Landry, D. and Guthrie, E.P. (2008). Glycobiology. 18, 799-805.
2.Roggentin, P. et al. (1988). FEBS Lett. 238, 31-34.
3.Guan, C. unpublished observations. New England Biolabs.
4.Morgan, W.T.J. and Elson, L.A. (1934). Biochem. J. 28, 988-995.
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
70,000 daltons。
无糖苷外切酶污染，无蛋白水解活性。
20,000 units/ml。
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
更多详细结构特异性请翻阅目录 256页
66,000 daltons。
无糖苷外切酶污染，无蛋白水解活性。
10,000 units/ml。