你了解中科百抗胎牛血清的灭活方法吗？

胎牛血清是从胎牛脐带血中提取的一种生物制品。它是一种黄褐色液体，主要由蛋白质、氨基酸、碳水化合物、脂类、矿物质和生长因子等组成。

胎牛血清在许多细胞培养和生物制药领域都被广泛应用。由于其含有丰富的营养物质和生长因子，胎牛血清可以为细胞提供必要的生长条件和支持细胞增殖。它被用作培养细胞和组织、制备疫苗、抗体和其他生物制剂的原料，以及用于生物医药研究和临床诊断。那么你了解中科百抗胎牛血清的灭活方法是什么吗？让我们一起来看看吧！

一般情况下，血清的灭活是指解冻后的血清，通过水浴加热的方式，将其加热至56℃维持30分钟，或者40℃维持20分钟，以达到灭活补体的目的。

灭活方法：

先将血清融化和混匀，热灭活时请将其置于 56 ℃ 水浴 30 分钟。为尽量减少热灭活对血清品质的影响，一次灭活的血清体积不宜过大。最好准备同样的容器装相等体积的水（与血清相同温度），灭活时将装有血清和水的容器同时放入 56 ℃ 水浴, 在装水的容器中放置温度计，加热过程中不时轻轻旋转混匀血清，当温度计显示达到 56 ℃ 左右，开始计时。56 ℃ 水浴后，建议马上转移到冰上降温。

注意事项：

·水面没过血清液面且管底不接触水浴锅底面；

·温度计需时不时晃动，且不接触管壁；

·初始温度应相同；

·两容器材质相同；

·液体体积相同；

·装水容器口用封口膜或保鲜膜封住，只露出温度计即可。

中科百抗胎牛血清来源于无菌采集的健康胎牛血清，适用于大部分原代细胞和部分免疫细胞，以及部分难贴壁细胞，典型代表有：UVEC、HUVEC；THP-1、REC-1、Raw264.7；293T、MRC-5。中科百抗胎牛血清在GMP净化车间全自动灌装生产，经过三级0.1μm过滤，在严格质量控制体系下，经过病毒、支原体、内毒素等多项测试，无细菌、真菌、病毒、支原体及黑胶虫等污染，内毒素含量≤ 10EU/ml，血红蛋白含量≤ 200mg/L，批量大、批间差小，具有高质量和高稳定性。

更多有关中科百抗胎牛血清的灭活方法，请咨询上海金畔生物科技有限公司客服！

上海金畔生物科技有限公司是美国源热灭活特级胎牛血清品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
美国源热灭活特级胎牛血清

美国源热灭活特级胎牛血清

产品名称：HyClone Characterized FBS, US Origin, 100 mL, Heat-inactivated;美国源热灭活特级胎牛血清

产品货号：SH30071.02HI;

处理方式：3×100 nm滤膜过滤;

地区来源：美国;

物种来源：;

内毒素水平：≤ 25 EU/mL;

血红素水平：< 25 mg/dL;

热灭活：灭活;

射线处理：未处理;

保存条件：≤-10℃;

运输方式：干冰运输;

详细介绍：Enhance growth of cells with U.S. sourced HyClone Characterized Fetal Bovine Serum (FBS).

HyClone characterized fetal bovine serum (FBS) is sourced in the USA. Complete traceability is provided back to the original source, as certified by the International Serum Industry Association (ISIA).

Additional traceability testing of premium HyClone fetal bovine serum is performed by our collaborator, Oritain.

HyClone FBS serum is low in antibodies and high in growth factors to support robust growth and division of cells, including embryonic stem cells.

HyClone characterized fetal bovine serum (FBS) is produced using true pool technology, which minimizes bottle-to-bottle variability. This FBS product is filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Each lot of FBS is subjected to virus panel testing according to 9 CFR 113.53. HyClone characterized FBS is assayed for many components, including gamma globulin, glucose, and IgG.

Additional treatments are available: HyClone FBS heat inactivated (HI) and HyClone FBS irradiated (IR). Additional testing, including EMEA-conforming testing, is also available.

Fetal bovine serum used in cell culture

Fetal bovine serum (FBS) is frequently added to cell culture media to promote cell growth and division. Because of its high content of growth factors and other nutrients that meet the metabolic needs of eukaryotic cells, FBS serum is frequently used to grow mammalian cells including embryonic stem cells.

HyClone FBS is certified for traceability

The manufacturing processes used to create HyClone serum are traceability certified by the International Serum Industry Association (ISIA). To further support product traceability, we were an early adopter of Scientific Traceability by Oritain to prove the country of origin of premium bovine sera products from the United States, Australia, and New Zealand. Premium bovine serum samples from different regions have been analyzed, and Oritain has created a database of the unique 'Origin Fingerprint' exclusive to each country of origin.

Have Confidence in Security of Supply with Secondary Sourcing

Fetal Bovine Serum from a single origin may be limited, so it’s important to qualify FBS products from other countries to increase your security of supply. HyClone Characterized FBS from United States (US), New Zealand (NZ) and Australia (AUS) have been evaluated and tested to show equivalent performance.