Nanocs Cholesterol PEG aldehyde is a lipid PEG derivative with amine reactive aldehyde group. Aldeyde PEG is a common N-terminal amine group pegylation reagent. The reaction between aldehyde group with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.
Physical Properties:
Off-white/white solid;
Soluble in warm water, DMSO, DMF;
Storage Conditions:
Store at -20 0C
Reaction Procedures:
Generally, a 5- to 10-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
PEG aldehyde stock solution: 50 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
Ethoxy or methoxyl silane functionalized polyethylene glycol, silane PEG (PEG-Si) is a surface reactive PEG derivative that can be used to modify glass, silica and other surfaces via the reaction between hydroxyl group and ethoxyl/methoxyl silane. Pegylation can greatly suppress the non-specific binding of charged molecules to the modified surfaces. And they have wide applications for medica device, biomems or biocompatible material development.
Physical Properties:
Off-white/white solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, desiccated. Silane PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.
Reaction Procedures:
Generally, silane PEG is first dissolved in a mixture of ethanol/water solution, then allow the solution to contact with oxidized glass or silica surface for 30 min to 2 hours. Under this condition, silane PEG should bind to the material covalently.
Materials Required:
Pegylation solution: Ethanol/water (w/w, 95%/5%);
Silane PEG stock solution, 10~50 mg/mL in pegylation solution, prepare in fresh.
Washing solution: Distilled water.
Reaction Steps:
1. Dissolve targeted materials in Pegylation buffer.
2. Add silane PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG needed for optimal conjugation;
3. Allow mixture agitates at room temperature for 30 min at room temperature or 2 hours at 4 -20 0C.
4. Wash out unreacted materials either by distilled water.
Maleimide PEG NHS is a heterobifunctional PEG derivative that can react with both thiol and amine groups. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. The maleimide group reacts with available –SH groups from naturally occurring or mutagenically derived cysteine residues. Maleimide groups react with sulfhydryl groups at pH 6.5~7.5 at room temperature to form stable thioether bonds.
Physical Properties:
Off-white/white solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
它 提供9种不同的加热模块。有三款模块都可放置24个微量管，适合0.5 ml，1.5 ml 和2.0 ml三种规格。此外Eppendorf还提供适合各种孔径的微孔板，11-11.9 mm试管，8 个15 ml Falcon®管，4 个50 ml Falcon®管，1.5-2 ml Cryo管，4块芯片玻片和4块 96 x 0.2 ml PCR管的模块。
Nanocs fluorescent labeled gold nanoparticles were made from monodispersed gold nanoparticles with narrow size distribution (<15%). Fluorescent molecules, from green to near infrared region, including fluorescein (FITC), rhodamine, Cy3, Cy5 or Alexa fluor dyes, were convalently attached to the gold surface. These fluorescently labeled gold nanoparticles are stable in aqueous buffer and is useful for generating various gold nanoparticle probes to detect various biomolecules in SEM, TEM, light microscopy and blotting.
Physical Properties:
Organe, red, purple or blue depends on composition;
Polystyrene Particles from Nanocs have narrow size distribution yet with multiple size selection from 20 nm to 10 microns. These particles are suspended in 1% aqeuous solution. Standard deviation of these particles is in the range of 10% of listed size parameter. These particles have wide applications in diagnostic reagents and bioassay development. They can be further modified with different surface coatings and functional groups. Nanocs surface modified polystyrene particles provide more choices for in-vitro or in-vivo applications of those nanoparticles. Customer conjugation and modification is available upon request.
Physical Properties:
1% suspension in aqueous solution, other concentration available upon request;
