上海金畔生物科技有限公司提供无血清培养基 ，欢迎访问官网了解更多产品信息和订购。
无血清培养基

无血清培养基（2X）

Additionally, it supports growth of chicken embryo fibroblasts under serum-free conditions. Virus production can then be conducted under serum-free conditions without the addition of albumin or other supplements.

The Diploid SRM system is composed of two different kits:

1. Diploid Growth Serum-Reduced Medium (SRM) kit: A basal medium and 100X growth supplement designed for cell expansion

2. Diploid Production Serum-Free Medium (SFM) kit (this page): A basal medium and 100X production supplement designed for virus production

Human cells have traditionally been used to manufacture a variety of vaccines, such as the varicella zoster, polio, smallpox, hepatitis A, measles, mumps, and rubella vaccines. Traditionally, the only way to culture these cells has been to use classical media with 10% serum for growth and 1–5% human albumin (human sourced or recombinant) for virus production. These are two of the highest cost components in vaccine production. The Diploid SRM system helps reduce or eliminate both components while maintaining high virus titers.

Cost savings:

Reduction or elimination of serum can significantly reduce cost for a vaccine manufacturing process. Premium FBS is expensive with high price volatility. Given the typical cost pressures on vaccine production, both economic drivers make vaccine manufacturing to a target price extremely challenging. Diploid Growth SRM reduces the impact of this volatility in price and offers a level of cost consistency not available with processes that use serum in high volumes.

In addition, serum requires frozen storage. A 10% serum concentration in growth media can result in high frozen storage requirements for a vaccine process. A process requiring 1,000 L of growth medium would require the frozen storage of 100 L of serum. The Diploid Growth SRM kit comes with a 100X frozen supplement, enabling an 80% reduction in frozen storage requirements. 1,000 L of Diploid Growth SRM would only need 20 L of frozen storage: 10 L for FBS and 10 L for the 100X supplement.

Albumin is commonly added to virus production media. With the Diploid Production SFM, viruses can be produced at comparable titers without any additional supplementation.

No adaptation required:

The Diploid SRM system was developed with existing cell banks in mind. Transitioning a vaccine process to a new medium can be challenging due to the need to adapt the cells to the new medium. For human cells such as MRC-5, there is the added risk of reaching the Hayflick limit due to the added passages necessary during cell adaptation and subsequent re-banking of the cells. The transition to the Diploid SRM system does not require adaptation or re-banking of cells when using the Diploid Growth SRM kit. Existing cGMP cells banked with serum may be thawed directly in Diploid Growth SRM supplemented with 1% FBS, allowing a simple transition to this medium.

Mitigate serum supply risk:

Obtaining a steady supply of serum can be challenging, especially if the serum required is from a BSE/TSE safe region such as New Zealand or Australia. Serum supply can fluctuate based on several environmental (drought, cattle disease) and economic (feed cost, dairy pricing) factors. With Diploid Growth SRM this dependence is substantially reduced due to the reduced serum requirements.

上海金畔生物科技有限公司是进口胎牛血清，射线照射品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
进口胎牛血清，射线照射

进口胎牛血清，射线照射

Product Name: Fetal Bovine Serum (FBS), US Origin, Gamma-irradiated.进口胎牛血清，射线照射

Product description: Product OverviewGamma irradiation is a process which involves exposing material to high energy gamma photons released by radioisotopes, such as cobalt 60. This energy is transferred from the photons to the products, and is responsible for organism inactivation by ionization of nucleic acid bonds. The irradiation of serum is intended to provide complete assurance of viral inactivation.

Isotype: Cocktail.

Host Species: Alligator.

Purification: Affinity purified.

全自动核酸提取仪的技术特点都在这了  

上海金畔生物科技有限公司提供231细胞专用培养基 ，欢迎访问官网了解更多产品信息和订购。
231细胞专用培养基

231细胞专用培养基

When supplemented with SMGS, Human Vascular Smooth Muscle Cell Basal Medium will support the plating and proliferation of smooth muscle cells at densities between 2.5 x 103 cells/cm2 and 1 x 105 cells/cm2. Additional applications for use may include primary isolation of human smooth muscle cells.

Human Vascular Smooth Muscle Cell Basal Medium is a basal medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. This medium is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support the plating and proliferation of normal human vascular smooth muscle cells, Medium 231 must be supplemented with Smooth Muscle Growth Supplement (SMGS). To induce differentiation of normal human vascular smooth muscle cells, Human Vascular Smooth Muscle Cell Basal Medium must be supplemented with Smooth Muscle Differentiation Supplement (SMDS).