标签归档：eagle

Gibco™ Basal Medium Eagle (BME)

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上海金畔生物科技有限公司提供Gibco™ Basal Medium Eagle (BME) ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Basal Medium Eagle (BME)

详情介绍

Gibco™ Basal Medium Eagle (BME)

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.


Product Intended Use：

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.


Dual-Site cGMP Manufacturing：

Gibco™ BME is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ BME product made in our Scotland facility (410100-026). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


Dulbecco′s Modified Eagle′s Medium – high glucose

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose:

form:powder.

technique(s):cell culture | mammalian: suitable.

components:glucose: high;NaHCO3: no;phenol red: yes;L-glutamine: yes;HEPES: no;sodium pyruvate: no.

Application:Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Dulbecco′s Modified Eagle′s Medium – high glucose

发表于由

上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose：

form:powder.

technique(s):cell culture | mammalian: suitable.

components:glucose: high;NaHCO3: no;phenol red: yes;L-glutamine: yes;HEPES: no;sodium pyruvate: no.

Application:Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.


Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur

详情介绍

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:glucose: 3.15 g/L;L-glutamine: yes;HEPES: no;sodium pyruvate: 0.055 g/L;phenol red: yes;NaHCO3: yes.

General description:Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application:Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites

to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment

to culture mouse squamous cell carcinoma (SCCVII) tumor cells


Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur

详情介绍

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:phenol red: no;L-glutamine: no;NaHCO3: yes;sodium pyruvate: 0.055 g/L;HEPES: 15 mM;glucose: 3.15 g/L.

General description:Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Dulbecco′s Modified Eagle′s Medium – high glucose

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:L-glutamine: no;glucose: high;sodium pyruvate: no;NaHCO3: yes;HEPES: no;phenol red: no.

Application:Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Dulbecco′s Modified Eagle′s Medium – low glucose

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – low glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – low glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – low glucose

form:powder.

technique(s):cell culture | mammalian: suitable.

solubility:H2O: soluble 10 g/L, clear.

components:HEPES: no;sodium pyruvate: yes;glucose: low;phenol red: no;L-glutamine: yes;NaHCO3: no.

Application:Dulbecco′s Modified Eagle′s Medium – low glucose is used in cell line culture (used in medium for the growth of cells) and for their maintenance. It is also used for tissue culture.

Dulbecco′s Modified Eagle′s Medium – low glucose

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – low glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – low glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – low glucose

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:phenol red: yes;HEPES: no;sodium pyruvate: yes;glucose: low;NaHCO3: yes;L-glutamine: no.

General description:This modification has been shown to improve the stability of the medium.

Minimum Essential Medium Eagle

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上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ，欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:NaHCO3: yes;sodium pyruvate: yes;glucose: yes;L-glutamine: no;HEPES: no;Hanks’ salts (2% CO2): no;phenol red: yes;stable glutamine: no;Earle’s salts (5% CO2): yes.

General description:Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Application:Minimum Essential Medium Eagle has been used to maintain:

immature cumulus-oocyte complexes from mice

human mesenchymal stem cells (MSC)

mouse pre-osteoblastic cell line

MEM Eagle培养基 sigma

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上海金畔生物科技有限公司提供MEM Eagle培养基 sigma ，欢迎访问官网了解更多产品信息和订购。
MEM Eagle培养基 sigma

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;L-glutamine: no;sodium pyruvate: no;NaHCO3: yes;phenol red: yes;HEPES: no.

General description:Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the c*tion of a wide variety of cells grown in monolayers.

Application:

Minimum Essential Medium Eagle has been used to culture:

HeLa cells;Madin-Darby canine kidney (MDCK) cells;chondrosarcoma cell line.

Minimum Essential Medium Eagle

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上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ，欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;L-glutamine: no;sodium pyruvate: no;NaHCO3: yes;phenol red: yes;HEPES: no.

General description:Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the c*tion of a wide variety of cells grown in monolayers.

Application:

Minimum Essential Medium Eagle has been used to culture:

HeLa cells;Madin-Darby canine kidney (MDCK) cells;chondrosarcoma cell line.

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixtur

详情介绍

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:HEPES: 15 mM;L-glutamine: no;NaHCO3: yes;phenol red: yes;sodium pyruvate: 0.055 g/L;glucose: 3.15 g/L.General description:

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.

Application:

DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Suitable for growth of wide range of cell types in low serum conditions.

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.

Dulbecco′s Modified Eagle′s Medium – high glucose

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | hybridoma: suitable;cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:glucose: high;NaHCO3: yes;sodium pyruvate: no;L-glutamine: no;phenol red: yes;HEPES: no.General description:

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application:

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Dulbecco′s Modified Eagle′s Medium – high glucose

发表于由

上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose:

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | hybridoma: suitable;cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:glucose: high;NaHCO3: yes;sodium pyruvate: no;L-glutamine: no;phenol red: yes;HEPES: no.General description:

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application:

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Dulbecco′s Modified Eagle′s Medium – high glucose

发表于由

上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | hybridoma: suitable;cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:glucose: high;NaHCO3: yes;sodium pyruvate: no;L-glutamine: no;phenol red: yes;HEPES: no.General description:

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application:

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Minimum Essential Medium Eagle

发表于由

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ，欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;NaHCO3: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;L-glutamine: no.Application:

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Minimum Essential Medium Eagle

发表于由

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ，欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;NaHCO3: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;L-glutamine: no.Application:

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Minimum Essential Medium Eagle

发表于由

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ，欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;NaHCO3: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;L-glutamine: no.Application:

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Dulbecco′s Modified Eagle′s Medium – high glucose

发表于由

上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose：

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:L-glutamine: no;phenol red: yes;sodium pyruvate: yes;NaHCO3: yes;HEPES: no;glucose: high.General description:

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application:

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

Dulbecco′s Modified Eagle′s Medium – high glucose

发表于由

上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – high glucose ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – high glucose

详情介绍

Dulbecco′s Modified Eagle′s Medium – high glucose

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:L-glutamine: no;phenol red: yes;sodium pyruvate: yes;NaHCO3: yes;HEPES: no;glucose: high.General description:

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application:

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.