Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:NaHCO3: yes;sodium pyruvate: yes;glucose: yes;L-glutamine: no;HEPES: no;Hanks’ salts (2% CO2): no;phenol red: yes;stable glutamine: no;Earle’s salts (5% CO2): yes.

General description:Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Application:Minimum Essential Medium Eagle has been used to maintain:

immature cumulus-oocyte complexes from mice

human mesenchymal stem cells (MSC)

mouse pre-osteoblastic cell line

Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;L-glutamine: no;sodium pyruvate: no;NaHCO3: yes;phenol red: yes;HEPES: no.

General description:Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the c*tion of a wide variety of cells grown in monolayers.

Application:

Minimum Essential Medium Eagle has been used to culture:

HeLa cells;Madin-Darby canine kidney (MDCK) cells;chondrosarcoma cell line.

Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;NaHCO3: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;L-glutamine: no.Application:

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;NaHCO3: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;L-glutamine: no.Application:

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;NaHCO3: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;L-glutamine: no.Application:

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:HEPES: no;NaHCO3: yes;L-glutamine: yes;Earle’s salts (5% CO2): yes;sodium pyruvate: no;

phenol red: yes.General description:Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to c*te normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for c*tion of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application:Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Minimum Essential Medium Eagle

上海金畔生物科技有限公司提供Minimum Essential Medium Eagle ,欢迎访问官网了解更多产品信息和订购。
Minimum Essential Medium Eagle

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:HEPES: no;NaHCO3: yes;L-glutamine: yes;Earle’s salts (5% CO2): yes;sodium pyruvate: no;

phenol red: yes.General description:Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to c*te normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for c*tion of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application:Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.