Gibco澳大利亚胎牛血清(10099141C)的原理

Gibco澳大利亚胎牛血清(10099141C)的原理

血清是基础培养基中极为重要的细胞生长和粘附因子、激素、脂质和矿物质来源。此外,血清还可调节细胞膜通透性,并可作为向细胞内运送脂质、酶、微量营养素和微量元素的载体。Gibco澳大利亚胎牛血清(10099141C)是一种常用的细胞培养试剂。那么你知道Gibco澳大利亚胎牛血清(10099141C)的原理是什么吗?让我们一起来看看吧!

Gibco澳大利亚胎牛血清(10099141C)是一种常用的细胞培养试剂,在细胞培养领域扮演着重要的角色。其原理涉及到胎牛血清中所含有的丰富生长因子、营养物质和其他生物活性成分,对细胞的生长、增殖和功能发挥着关键作用。

Gibco澳大利亚胎牛血清所含的生长因子是促进细胞增殖和生长的关键因素。这些生长因子包括但不限于胰岛素样生长因子(IGF)、表皮生长因子(EGF)、成纤维细胞生长因子(FGF)等,它们通过调控细胞的代谢、信号传导和基因表达,直接影响细胞的生长和分化过程。

其次,胎牛血清中丰富的营养物质为细胞提供了生长所需的能量和基础物质。这些营养物质包括葡萄糖、氨基酸、维生素、矿物质等,为细胞代谢活动提供支持,保证细胞在培养条件下正常生长和功能维持。

此外,Gibco澳大利亚胎牛血清中还含有一系列的蛋白质、脂质、激素和细胞因子等生物活性成分,这些成分能够调节细胞的信号传导通路、细胞凋亡和细胞周期等重要生物学过程,从而影响细胞的功能和特性。

Gibco澳大利亚胎牛血清作为细胞培养中不ke或缺的试剂,其原理在于提供丰富的生长因子、营养物质和其他生物活性成分,为细胞的生长、增殖和功能发挥关键作用。通过与细胞相互作用,胎牛血清为细胞提供了适宜的生长环境,促进细胞在培养条件下的健康生长和稳定传代,为科研和医学领域的细胞实验提供了坚实的基础。

主要功能:

1.提供维持细胞指数生长的激素。

2.酸碱缓冲液作用。

3.细胞贴壁、铺展在塑料基质上所需因子来源。

4.提供蛋白酶抑制剂,使细胞传代时细胞不受伤害5提供结合蛋白,结合或调控维生素、脂类、金属和其他激素的结合物质的活力;与有毒金属和热原质结合,解毒作用。

产品选购:

货号

产品名称

规格

10099141C

澳大利亚胎牛血清

500 mL

10099158

澳大利亚胎牛血清

1,000 mL

A3161001C

澳大利亚胎牛血清

50 mL

A3161002C

澳大利亚胎牛血清

10 x 50 mL

Gibco含核苷的基础MEMα培养基(C12571500BT )

上海金畔生物科技有限公司提供Gibco含核苷的基础MEMα培养基(C12571500BT ) ,欢迎访问官网了解更多产品信息和订购。
Gibco含核苷的基础MEMα培养基(C12571500BT )

Gibco含核苷的基础MEMα培养基广泛用于哺乳动物细胞培养以及转染 DHFR 阴性细胞的筛选。MEM α 可用于各种悬浮和贴壁的哺乳动物细胞,包括角质形成细胞、原代大鼠星形胶质细胞和人黑色素瘤细胞。针对广泛的细胞培养应用,提供了多种组分的 MEM α 改良培养基。

MEM α 是zui低必需培养基 (MEM) 的改良版本,其中包含非必需氨基酸、丙酮酸钠、硫辛酸、维生素 B12、生物素和抗坏血酸。MEM α 还提供不含核苷的版本,可用作 DG44 和其他 DHFR 阴性细胞的筛选培养基。该产品由 Earle’s 平衡盐制成。MEM α 不含蛋白质、脂质或生长因子。因此,MEM α 需要补充剂,通常添加 10% 胎牛血清 (FBS)。MEM α 使用碳酸氢钠缓冲系统 (2.2 g/L),因此需要 5–10% CO2 环境来维持生理 pH 值。

产品属性:

细胞系:HeLa、BHK-21、293、HEP-2、HT-1080、MCF-7 和成纤维细胞

细胞类型:原代大鼠星形胶质细胞

分类:无动物来源

浓度:1 X

形式:液体

产品类型:MEM α(zui低必需培养基 α)

添加剂:低糖, 谷氨酰胺, 酚红, 丙酮酸钠, 核糖核苷, 脱氧核糖核苷

货号

产品名称

规格

C12571500BT

BASIC MEM α, Nucleosides

500 mL

 

现货供应:Gibco胰蛋白酶-EDTA (0.25%),含酚红(25200072/25200056)

现货供应:Gibco胰蛋白酶-EDTA (0.25%),含酚红(25200072/25200056)

胰蛋白酶是一种消化酶,可分解许多脊椎动物消化系统中的蛋白质,它存在于胰液中。质膜上的蛋白质负责多种功能,这些功能对于维持细胞的正常生理活动至关重要。一些质膜蛋白(如钙粘蛋白家族)是粘附蛋白,可作为锚,将细胞骨架蛋白连接到细胞外基质。这有助于细胞粘附和细胞迁移。EDTA也与胰蛋白酶一起添加到细胞培养物中,以螯合培养基中的二价离子。钙和镁离子可能抑制胰蛋白酶的作用。在细胞培养物中,可以将胰蛋白酶添加到培养基中,通过消化粘附蛋白从培养皿表面释放贴壁细胞,胰蛋白酶还通过消化粘附蛋白从聚集体中释放细胞。最终,胰蛋白酶有助于从细胞培养物中获得单个细胞,促进细胞的下游处理。

Gibco 胰蛋白酶溶液经过 pH、渗透压、无菌性和性能测试。此外,在生产之前,原材料均经过电子束辐照验证和内毒素、PPVPCV 1/2、支原体、细菌、真菌和病毒污染检测,还进行了多种活性测定、灰含量分析和水含量分析。下面让我们来看看Gibco胰蛋白酶-EDTA (0.25%),含酚红的产品属性吧!

螯合剂:EDTA

细胞类型:哺乳动物细胞

加有添加剂:酚红,EDTA

分类:动物来源

产品类型:细胞培养解离试剂

浓度:1 X

形式:液体

渗透压:270 – 320 mOsm/kg

无菌:无菌过滤

pH7.1 – 8

经过测试:体外生物测定

运输条件:湿冰

上海金畔生物科技有限公司现货供应Gibco胰蛋白酶-EDTA (0.25%),含酚红(25200072/25200056),欢迎选购!

现货供应:Gibco青霉素-链霉素双抗溶液Penicillin-Streptomycin(15140122)

现货供应:Gibco青霉素-链霉素双抗溶液Penicillin-Streptomycin(15140122)

Gibco青霉素链霉素双抗溶液是细胞培养中最常被使用的抗生素之一,由青霉素和链霉素混合而成,其分别作用于革兰氏阴性菌及阳性菌,配合使用可以有效预防细胞培养中的细菌污染,其中青霉素最初由青霉属真菌中纯化而来,通过直接干扰细菌细胞壁的周转,间接触发酶的释放,从而进一步改变细胞壁,导致细菌死亡。链霉素最初则链霉菌中纯化而来。其通过与细菌核糖体30S亚基结合,进而抑制蛋白质合成并导致细菌死亡。青霉素阻碍细胞壁合成,导致细胞泄漏;链霉素作用于核糖体,阻碍蛋白翻译。二者作用位点不同。自然环境下(不考虑人为产生的抗药性),对青霉素不敏感的微生物绝大多数对链霉素敏感,反之亦然。两种抗生素搭配可以控制几乎全部常见细菌。下面让我们一起来看看Gibco青霉素链霉素双抗溶液的产品属性吧!

浓度:100 X

产品类型:青霉素链霉素

形式:液体

无菌:无菌过滤

适用于(应用):防止细胞培养受到污染

有效期:12 个月

数量:100 mL

运输条件:干冰

上海金畔生物科技有限公司提供Gibco青霉素链霉素双抗溶液Penicillin-Streptomycin(15140122),欢迎选购!

现货供应:Gibco RPMI 1640培养基(C11875500BT)

现货供应:Gibco RPMI 1640培养基(C11875500BT)

 Gibco1962年成立至今60余年,一直致力于以世界yi流的研发实力、质量体系和强健的供应链赋能生物制药企业的研发和生产。作为细胞培养基的市场ling导者,一直在制定和yin领标准。在生命科学行业中,Gibco在科学出版物中被引用的次数位居前列,深受广大研发者的认可,其培养基质量和一致性经得起长时间和大量试验的考验。

RPMI-1640(Roswell Park Memorial Institute medium 1640)是一种由Roswell Park纪念研究所开发的细胞培养基,适用于淋巴细胞、骨髓细胞、白血病细胞等多种类型的哺乳动物细胞系的培养。RPMI-1640中含有较低浓度的葡萄糖(2000mg/L)、氨基酸、维生素和微量元素等营养物质。此外,RPMI-1640还添加了缓冲剂HEPES和碳酸氢盐,能够保持细胞在较窄的pH范围内正常生长。下面让我们一起来看看Gibco RPMI 1640培养基(C11875500BT)的产品属性吧!

细胞系:HeLaJurkatMCF-7PC-12PBMC、星形胶质细胞和癌细胞

分类:无动物来源

细胞类型:白血病细胞

形式:液体

血清水平:标准血清添加

浓度:1 X

有效期:12个月

添加剂:谷氨酰胺, 酚红

无菌:无菌过滤

不含添加剂:不含 HEPES

运输条件:室温

上海金畔生物科技有限公司现货供应Gibco RPMI 1640培养基(C11875500BT),欢迎选购!

现货供应:Gibco DMEM高糖培养基(C11995500BT)

现货供应:Gibco DMEM高糖培养基(C11995500BT)

Gibco是赛默飞世尔科技的品牌组合中的重要一员。自1962年品牌创立以来,Gibco对高品质的追求始终如一,坚持一以贯之的高标准,以wu与伦比的创新、可靠性和可追溯性为科研和生物药物的生产提供了坚实的质量保障,一直是细胞培养技术和应用科学的先驱。

作为市场的领dao者,Gibco是科研、生物技术和制药研究专业人士gong认的行业标准。此外,凭借广泛的创新与可靠的产品、服务、专业知识和支持,Gibco也在科学家与他们所需的各种资源之间,建立起紧密的连接,推动他们实现*研究和*生物工艺的成功。

DMEM高糖培养基是在MEM培养基基础上改良而来,Gibco DMEM高糖培养基(C11995500BT)目前已广泛应用于各种细胞的培养。DMEM高糖培养基普遍应用于生长快、粘附性低的细胞。高糖DMEM可以为细胞提供充足的碳源,适用于代谢快的细胞,如胚胎干细胞 (ES细胞) 等,可用于多种哺乳动物原代细胞的培养,例如原代成纤维细胞、神经细胞、神经胶质细胞、HUVEC、平滑肌细胞等,也可以用于一些细胞系的培养,例如Hela293Cos-7PC-12等。下面让我们来看看Gibco DMEM高糖培养基(C11995500BT)的产品属性吧!

葡萄糖:高糖

血清补充:标准血清补充

丙酮酸钠添加剂:丙酮酸钠

HEPES缓冲液:无HEPES

形式:液体

酚红指示剂:酚红

谷氨酰胺:L-谷氨酰胺

运输条件:室温

应用:哺乳动物细胞培养

  上海金畔生物科技有限公司现货供应Gibco DMEM高糖培养基(C11995500BT),欢迎选购!

现货供应:Gibco南美胎牛血清FBS(10270106)

现货供应:Gibco南美胎牛血清FBS(10270106)

1962年以来,Gibco对高品质的追求始终如一,坚持一以贯之的高标准,以wu与伦比的创新、可靠性和可追溯性为您的科研和生产提供坚实的质量保障,使科学家能够安心专注于解决比细胞培养更重要的问题。如今,最初位于纽约格兰德岛的Gibco生产基地只是全球众多Gibco细胞培养产品的生产基地之一,在支持生命科学领域科研人员的同时,还是生物制药行业的供应商。

半个世纪以来,Gibco血清等细胞培养产品保障始终如一的质量交付,助力和见证了生命科学领域的非凡突破。

胎牛血清是细胞培养中用量的天然培养基,含有丰富的细胞生长必须的营养成份,常用于动物细胞的体外培养,具有极为重要的功能。Gibco南美胎牛血清FBS(10270106)来源于南美洲,适合于培养各种常规的比较容易养活的细胞株和癌细胞株,Gibco南美胎牛血清为您的实验保驾护航。下面让我们一起来看看Gibco南美胎牛血清FBS(10270106)的产品属性吧!

血清等级:标准,普通

地区:南美洲

物种:胎牛

处理方式:标准,三次0. 1 um滤膜过滤

昆虫细胞:sf9细胞培养验证通过

内毒素水平:≤50 EU/ml

血红素水平:≤30 mg/dl (一般 ≤25 mg/dl)

热灭活:未灭活

上海金畔生物科技有限公司现货供应Gibco南美胎牛血清FBS(10270106),欢迎选购!

 

 

 

现货供应:Gibco N-2 添加剂100X(17502048)

现货供应:Gibco N-2 添加剂100X(17502048)

Gibco是科研、生物技术和制药研究专业人士gong认的行业标准。此外,凭借zui广泛的创新与可靠的产品、服务、专业知识和支持,Gibco也在科学家与他们所需的各种资源之间,建立起紧密的连接,推动他们实现*研究和*生物工艺的成功。

1962年品牌创立以来,Gibco对高品质的追求始终如一,坚持一以贯之的高标准,以wu与伦比的创新、可靠性和可追溯性为您的科研和生产提供坚实的质量保障。

N2 无血清培养基添加物是化学成分确定的,不含血清的,常用于神经细胞无血清培养的补充剂,是一种优化的无血清培养基添加物。主要被推荐用于成神经细胞瘤以及周围神经系统(PNS)与中枢神经系统(CNS)来源的有丝分裂后期的神经元的生长和表达。原料均选用自产或者经过严格检测和纯化的低内毒素高纯药用级别原料,严格的质量检测程序,远gao于同行业要求指标,产品性能突出,指标优于进口。满足您的实验与研究需求。Gibco N-2 添加剂仅供研究使用,不适用于任何动物或人类的诊断用途。下面让我们一起来看看Gibco N-2 添加剂100X(17502048)的基本属性吧!

细胞类型:神经细胞

浓度:100 X

分类:无血清

培养类型:哺乳动物细胞培养

产品类型:细胞培养添加剂

形式:液体

血清水平:无血清

pH6 – 8

无菌:无菌

运输条件:干冰

测试:内毒素、性能、无菌性测试

  上海金畔生物科技有限公司现货供应GibcoN-2添加剂100X(17502048),欢迎选购!

 

现货供应:Gibco澳洲优质胎牛血清(A5669701)

现货供应:Gibco澳洲优质胎牛血清(A5669701)

1962年,伦纳德·海弗里克 (Leonard Hayflick) 取得一项重大发现,即正常人类细胞在体外培养中的复制能力有限。这一发现tui翻了人们对培养细胞潜在永生性的长期信念,并对生命科学研究产生了深远的影响。

同年,两位在纽约格兰德岛的车库工作的生物学家BobEarline Ferguson认识到为科学研究提供动物血清的商业潜力。从这个小小的开始,Gibco血清越升至支持全球生命科学研究的前沿产品。

胎牛血清 (FBS) 是来自胎牛的凝结血液的液体部分,不含细胞、纤维蛋白和凝血因子,但含有大量对细胞生长至关重要的营养和大分子因子。牛血清白蛋白是FBS 的主要成分。FBS 中的生长因子对于培养细胞的维持和生长至关重要。FBS 还含有多种小分子,如氨基酸、糖、脂质和激素。Gibco胎牛血清(A5669701)采用了严格的无菌采集技术以确保内毒素和血红蛋白维持在低水平,Gibco尽可能在原产地生产血清,最大限度地减少细菌积聚,每个批次的产品均会进行多达90项质量控制检测,满足您的实验研究需求。下面一起来看看Gibco澳洲优质胎牛血清(A5669701)的产品基本属性吧!

种属:牛

年龄:胎儿

产品类型:胎牛血清

产品规格:瓶

无菌:无菌

灭菌方法:三重过滤,0.1μm

级别:Premium Plus

测试:多达70项质量检测,包括EMA病毒检测;USP/EP支原体、内毒素、性能;生化/激素分析;Oritain指纹

内毒素浓度:≤5 EU/mL

血红蛋白浓度:≤20 mg/dL

运输条件:干冰

有效期:5

上海金畔生物科技有限公司现货供应Gibco澳洲优质胎牛血清(A5669701),欢迎选购!

Gibco L-15 培养基,粉末代理商

上海金畔生物科技有限公司是Gibco L-15 培养基,粉末代理商 ,欢迎访问官网了解更多产品信息和订购。
Gibco L-15 培养基,粉末

详情介绍

Gibco™ Leibovitz's L-15 Medium, powder(Gibco L-15 培养基,粉末

Using Leibovitz's L-15 Medium:

L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Powder forms of Gibco™ L-15 cell culture medium require pH adjustment and filtration at the time of preparation (see protocol for details).


cGMP Manufacturing and Quality System:

Gibco™ L-15 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ L-15 product made in our Scotland facility (41300-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ DMEM, high glucose, HEPES代理商

上海金畔生物科技有限公司是Gibco™ DMEM, high glucose, HEPES代理商 ,欢迎访问官网了解更多产品信息和订购。
Gibco™ DMEM, high glucose, HEPES

详情介绍

Gibco™ DMEM, high glucose, HEPES

Using DMEM:

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (42430-082). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco DMEM高糖培养基大量

上海金畔生物科技有限公司提供Gibco DMEM高糖培养基大量 ,欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基大量

详情介绍

Gibco DMEM高糖培养基大量

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.


Product Use:

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


cGMP Manufacturing and Quality System:

Gibco™ DMEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.


DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Gibco DMEM高糖培养基现货

上海金畔生物科技有限公司提供Gibco DMEM高糖培养基现货 ,欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基现货

详情介绍

Gibco DMEM高糖培养基现货

Using DMEM:

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

RPMI 1640培养基Gibco

上海金畔生物科技有限公司提供RPMI 1640培养基Gibco ,欢迎访问官网了解更多产品信息和订购。
RPMI 1640培养基Gibco

详情介绍

RPMI 1640 Medium,powder,HEPES(RPMI 1640培养基Gibco)

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.


Product Intended Use:

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).


cGMP Manufacturing and Quality System:

Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ RPMI 1640 product made in our Scotland facility (13018-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Gibco Opti-MEM™ 减血清培养基

上海金畔生物科技有限公司提供Gibco Opti-MEM™ 减血清培养基 ,欢迎访问官网了解更多产品信息和订购。
Gibco Opti-MEM™ 减血清培养基

详情介绍

Gibco Opti-MEM™ 减血清培养基

Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.


cGMP Manufacturing and Quality System:

For supply chain continuity, we manufacture Gibco™ Opti-MEM™ at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.


Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation

Gibco MEM Medium

上海金畔生物科技有限公司提供Gibco MEM Medium ,欢迎访问官网了解更多产品信息和订购。
Gibco MEM Medium

详情介绍

Gibco MEM Medium

Using MEM:

MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM product made in our Scotland facility (31095-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco DMEM高糖培养基 500ml

上海金畔生物科技有限公司提供Gibco DMEM高糖培养基 500ml ,欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基 500ml

详情介绍

Gibco DMEM高糖培养基 500ml

Using DMEM:

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco RPMI 1640培养基大量

上海金畔生物科技有限公司提供Gibco RPMI 1640培养基大量 ,欢迎访问官网了解更多产品信息和订购。
Gibco RPMI 1640培养基大量

详情介绍

Gibco RPMI 1640培养基大量

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-059). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco GMEM培养基

上海金畔生物科技有限公司提供Gibco GMEM培养基 ,欢迎访问官网了解更多产品信息和订购。
Gibco GMEM培养基

详情介绍

Gibco GMEM培养基

Using Glasgow's MEM:

Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


Dual-site cGMP manufacturing:

Glasgow's MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Glasgow's MEM product made in our Scotland facility (21710-025). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco DMEM高糖培养基,不含磷酸盐

上海金畔生物科技有限公司提供Gibco DMEM高糖培养基,不含磷酸盐 ,欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基,不含磷酸盐

详情介绍

Gibco DMEM高糖培养基,不含磷酸盐

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.


Product Use:

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


cGMP Manufacturing and Quality System:

Gibco™ DMEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.


DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.