标签归档：高糖

DMEM低糖培养基与DMEM高糖培养基有何区别？

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DMEM低糖培养基与DMEM高糖培养基有何区别？

DMEM培养基是一种含各种氨基酸和葡萄糖的培养基，是在MEM培养基的基础上研制的。与MEM相比，DMEM增加了各种成分用量，同时又分为高糖型(低于4500mg/L)和低糖型(低于1000mg/L)。那么你知道DMEM低糖培养基与DMEM高糖培养基有何区别吗？让我们一起来看看吧！

一、DMEM高糖培养基

DMEM高糖培养基是在MEM培养基基础上改良而来，目前已广泛应用于各种细胞的培养。DMEM高糖培养基普遍应用于生长快、粘附性低的细胞。可用于多种哺乳动物原代细胞的培养，例如原代成纤维细胞、神经细胞、神经胶质细胞、HUVEC、平滑肌细胞等，也可以用于一些细胞系的培养，例如Hela、293、Cos-7及PC-12等。

二、DMEM低糖培养基

DMEM低糖培养基是一种应用十分广泛的培养基，可用于许多哺乳动物细胞培养。DMEM低糖培养基适合代谢作用慢、依赖性贴壁细胞的培养。

三、DMEM高糖与DMEM低糖的区别

低糖DMEM适合用于培养代谢作用较慢的细胞，高糖DMEM可以为细胞提供充足的碳源，适用于代谢快的细胞，如胚胎干细胞 (ES细胞) 等。同时，低糖的培养基有助于维持细胞的低分化状态，高糖培养基有助于细胞分化。此外，做单抗细胞融合时，一般也使用低糖培养基，因为高糖使细胞生长过快，不利于融合细胞的染色体稳定。

更多有关DMEM低糖培养基与DMEM高糖培养基的区别，请联系上海金畔生物科技有限公司。

现货供应：Gibco DMEM高糖培养基(C11995500BT)

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现货供应：Gibco DMEM高糖培养基(C11995500BT)

Gibco是赛默飞世尔科技的品牌组合中的重要一员。自1962年品牌创立以来，Gibco对高品质的追求始终如一，坚持一以贯之的高标准，以wu与伦比的创新、可靠性和可追溯性为科研和生物药物的生产提供了坚实的质量保障，一直是细胞培养技术和应用科学的先驱。

作为市场的领dao者，Gibco是科研、生物技术和制药研究专业人士gong认的行业标准。此外，凭借广泛的创新与可靠的产品、服务、专业知识和支持，Gibco也在科学家与他们所需的各种资源之间，建立起紧密的连接，推动他们实现*研究和*生物工艺的成功。

DMEM高糖培养基是在MEM培养基基础上改良而来，Gibco DMEM高糖培养基(C11995500BT)目前已广泛应用于各种细胞的培养。DMEM高糖培养基普遍应用于生长快、粘附性低的细胞。高糖DMEM可以为细胞提供充足的碳源，适用于代谢快的细胞，如胚胎干细胞 (ES细胞) 等，可用于多种哺乳动物原代细胞的培养，例如原代成纤维细胞、神经细胞、神经胶质细胞、HUVEC、平滑肌细胞等，也可以用于一些细胞系的培养，例如Hela、293、Cos-7及PC-12等。下面让我们来看看Gibco DMEM高糖培养基(C11995500BT)的产品属性吧！

葡萄糖：高糖

血清补充：标准血清补充

丙酮酸钠添加剂：丙酮酸钠

HEPES缓冲液：无HEPES

形式：液体

酚红指示剂：酚红

谷氨酰胺：L-谷氨酰胺

运输条件：室温

应用：哺乳动物细胞培养

  上海金畔生物科技有限公司现货供应Gibco DMEM高糖培养基(C11995500BT)，欢迎选购！

Gibco DMEM高糖培养基大量

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上海金畔生物科技有限公司提供Gibco DMEM高糖培养基大量 ，欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基大量

详情介绍

Gibco DMEM高糖培养基大量

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.


Product Use：

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


cGMP Manufacturing and Quality System：

Gibco™ DMEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.


DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Gibco DMEM高糖培养基现货

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上海金畔生物科技有限公司提供Gibco DMEM高糖培养基现货 ，欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基现货

详情介绍

Gibco DMEM高糖培养基现货

Using DMEM：

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco DMEM高糖培养基 500ml

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上海金畔生物科技有限公司提供Gibco DMEM高糖培养基 500ml ，欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基 500ml

详情介绍

Gibco DMEM高糖培养基 500ml

Using DMEM：

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM高糖培养基报价

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上海金畔生物科技有限公司提供DMEM高糖培养基报价 ，欢迎访问官网了解更多产品信息和订购。
DMEM高糖培养基报价

详情介绍

DMEM高糖培养基报价

Using DMEM：

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

DMEM高糖干粉培养基

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上海金畔生物科技有限公司提供DMEM高糖干粉培养基 ，欢迎访问官网了解更多产品信息和订购。
DMEM高糖干粉培养基

详情介绍

DMEM高糖干粉培养基

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.


cGMP Manufacturing and Quality System：

Gibco™ DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ DMEM product made in our Grand Island facility (12100-061). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.


DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.


Gibco DMEM高糖培养基，不含磷酸盐

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上海金畔生物科技有限公司提供Gibco DMEM高糖培养基，不含磷酸盐 ，欢迎访问官网了解更多产品信息和订购。
Gibco DMEM高糖培养基，不含磷酸盐

详情介绍

Gibco DMEM高糖培养基，不含磷酸盐

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.


Product Use:

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.


cGMP Manufacturing and Quality System:

Gibco™ DMEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.


DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Gibco DMEM培养基高糖报价

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上海金畔生物科技有限公司提供Gibco DMEM培养基高糖报价 ，欢迎访问官网了解更多产品信息和订购。
Gibco DMEM培养基高糖报价

详情介绍

Gibco DMEM培养基高糖报价

Using DMEM：

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical DMEM product made in our Scotland facility (41965-039). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Dulbecco′s Modified Eagle′s Medium – 高糖

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上海金畔生物科技有限公司提供Dulbecco′s Modified Eagle′s Medium – 高糖 ，欢迎访问官网了解更多产品信息和订购。
Dulbecco′s Modified Eagle′s Medium – 高糖

详情介绍

Dulbecco′s Modified Eagle′s Medium – 高糖:

sterility:sterile-filtered.

form:liquid.

components:glucose: high;L-glutamine: yes;NaHCO3: yes;sodium pyruvate: no;phenol red: yes;HEPES: no.General description:This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.Application:Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.

DMEM/高糖（不含HEPES） 货号: H9008 规格: 500ml

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上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

DMEM/高糖（不含HEPES）

产品货号: H9008

产品规格: 500ml

目录价（元）:95

大包装询价


产品概述：
产品参数
含高糖、L-谷氨酰胺、酚红、丙酮酸钠，不含 HEPES。

储存条件
2-8℃避光保存，有效期见外包装。

产品介绍
DMEM（Dulbecco 改良 Eagle 培养基）是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。已经在 DMEM 中培养成功的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC、平滑肌细胞，以及 HeLa、293、Cos-7 和 PC-12 等细胞系。本品为 DMEM 高糖培养基，更适合代谢作用较快的细胞的培养。

注意事项
1. 培养基长期放置，需要 2-8℃避光保存，请勿低温冻存。
2. 使用时注意无菌操作。
3. 培养基中添加的 L-谷氨酰胺在溶液中不稳定、易分解，长期放置后使用若发现细胞生长变慢可在使用前加入适量的 L-谷氨酰胺，来维持细胞的正常代谢。
说明书：

DMEM/高糖（不含HEPES） 货号: H9008 规格: 500ml UE-H9008    

DMEM/高糖（不含酚红） 货号: H9009 规格: 500ml

发表于由

上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品

DMEM/高糖（不含酚红）

产品货号: H9009

产品规格: 500ml

目录价（元）:95

大包装询价


产品概述：

产品参数
含高糖、L-谷氨酰胺、HEPES，不含丙酮酸钠、酚红。

储存条件
2-8℃避光保存，有效期见见外包装。

产品介绍

DMEM（Dulbecco改良Eagle培养基）是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。已经在DMEM中培养成功的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC、平滑肌细胞，以及HeLa、293、Cos-7和PC-12等细胞系。本品为DMEM高糖培养基，更适合代谢作用较快的细胞的培养。

注意事项
1. 培养基长期放置，需要2-8℃避光保存，请勿低温冻存。
2. 使用时注意无菌操作。
3. 培养基中添加的L-谷氨酰胺在溶液中不稳定、易分解，长期放置后使用若发现细胞生长变慢可在使用前加入适量的L-谷氨酰胺，来维持细胞的正常代谢。


说明书：

DMEM/高糖（不含酚红） 货号: H9009 规格: 500ml UE-H9009    

DMEM高糖培养基 Gibco

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上海金畔生物科技有限公司提供DMEM高糖培养基 Gibco ，欢迎访问官网了解更多产品信息和订购。
DMEM高糖培养基 Gibco

详情介绍

产品名称：DMEM，高糖

产品简介：DMEM（Dulbecco 改良 Eagle 培养基）是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物细胞生长。已经在 DMEM 中培养成功的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC、平滑肌细胞，以及 HeLa、293、Cos-7 和 PC-12 等细胞系。

改良的DMEM含有：高葡萄糖、酚红、bing tong 酸钠，不含：HEPES

DMEM的使用：DMEM 与其他培养基的不同之处在于其含有相当于初始 Eagle 必需培养基 4 倍浓度的氨基酸和vitamin。DMEM 最初采用低浓度葡萄糖 (1 g/L) 和bing tong 酸钠配制，但通常在含/不含bing tong 酸钠的高糖环境下使用。DMEM 不含蛋白、脂质或生长因子。因此，DMEM 需要补充剂，通常添加 10% 胎牛血清 (FBS)。DMEM 使用碳酸qing钠缓冲系统 (3.7 g/L)，因此需要 5–10% CO

2 的环境来维持生理 pH 值。

产品规格：