血清必须要热灭活吗?

血清必须要热灭活吗?

血清是生长和附着因子、矿物质、脂质和激素的来源,是细胞培养的重要组分。血清能调节细胞膜的通透性,同时能作为载体携带酶、脂质、酶和微量元素等进入细胞。在使用的过程中,到底是选择没有灭活的血清?还是选择热灭活血清?有啥区别吗?

热灭活可以破坏血清中补体的活性。哺乳动物血清中存在的补体蛋白参与细胞的溶解,收缩平滑肌,刺激细胞和血小板释放组胺,并激活淋巴细胞和巨噬细胞。有研究比较了正常热灭活胎牛血清对各种细胞株的生长能力影响(如下图所示),发现11株细胞中,热灭活对6株细胞有负面影响(红框)、3株无影响(绿框)、2株有微弱正面改善(黄框),因此在多数细胞培养中血清不需要热灭活,热灭活对细胞生长没有明显的促进作用,反而还会影响血清质量,使细胞的生长速率降低。因此如果不是非必须,可不必做血清热灭活这一处理。所以一般的细胞选择没有灭活的血清即可。

图1 使用未灭活或灭活血清的细胞生长状况

但是有些细胞需要选择热灭活血清,如胚胎干细胞(ESC)、平滑肌细胞、免疫细胞和昆虫细胞培养。

上海金畔生物科技有限公司提供热灭活/非热灭活各式胎牛和新生牛血清以供实验的选择,产品详情,请咨询客服小姐姐。

美国源胎牛血清,热灭活品牌代理商

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美国源胎牛血清,热灭活

详情介绍

美国源胎牛血清,热灭活

Product Name: Fetal Bovine Serum (FBS), US Origin, Heat Inactivated.美国源胎牛血清,热灭活

Product description: 

FBS sourced in the United States is used in a broad range of cell culture applications. Sterile-filtered also suitable for insect cell culture.Promotes steady, healthy cell growth.

Isotype: Cocktail.

Host Species: Alligator.

Purification: Affinity purified.

Storage Instructions: -20ºC.

特级胎牛血清(照射、热灭活)品牌代理商

上海金畔生物科技有限公司是特级胎牛血清(照射、热灭活)品牌代理商 ,欢迎访问官网了解更多产品信息和订购。
特级胎牛血清(照射、热灭活)

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特级胎牛血清(照射、热灭活)

产品名称:HyClone Characterized FBS, US Origin, 500 mL, Irradiated 25-40kGy, Heat-inactivated;特级胎牛血清(照射、热灭活)

产品货号:SH30071.03IH25-40;

处理方式:3×100 nm滤膜过滤;

地区来源:美国;

物种来源:;

内毒素水平:≤ 25 EU/mL;

血红素水平:< 25 mg/dL;

热灭活:灭活;

射线处理:处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:Enhance growth of cells with U.S. sourced HyClone Characterized Fetal Bovine Serum (FBS).


HyClone characterized fetal bovine serum (FBS) is sourced in the USA. Complete traceability is provided back to the original source, as certified by the International Serum Industry Association (ISIA).

Additional traceability testing of premium HyClone fetal bovine serum is performed by our collaborator, Oritain.

HyClone FBS serum is low in antibodies and high in growth factors to support robust growth and division of cells, including embryonic stem cells.

HyClone characterized fetal bovine serum (FBS) is produced using true pool technology, which minimizes bottle-to-bottle variability. This FBS product is filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Each lot of FBS is subjected to virus panel testing according to 9 CFR 113.53. HyClone characterized FBS is assayed for many components, including gamma globulin, glucose, and IgG.


Additional treatments are available: HyClone FBS heat inactivated (HI) and HyClone FBS irradiated (IR). Additional testing, including EMEA-conforming testing, is also available.


Fetal bovine serum used in cell culture

Fetal bovine serum (FBS) is frequently added to cell culture media to promote cell growth and division. Because of its high content of growth factors and other nutrients that meet the metabolic needs of eukaryotic cells, FBS serum is frequently used to grow mammalian cells including embryonic stem cells.


HyClone FBS is certified for traceability

The manufacturing processes used to create HyClone serum are traceability certified by the International Serum Industry Association (ISIA). To further support product traceability, we were an early adopter of Scientific Traceability by Oritain to prove the country of origin of premium bovine sera products from the United States, Australia, and New Zealand. Premium bovine serum samples from different regions have been analyzed, and Oritain has created a database of the unique 'Origin Fingerprint' exclusive to each country of origin.


Have Confidence in Security of Supply with Secondary Sourcing

Fetal Bovine Serum from a single origin may be limited, so it’s important to qualify FBS products from other countries to increase your security of supply. HyClone Characterized FBS from United States (US), New Zealand (NZ) and Australia (AUS) have been evaluated and tested to show equivalent performance. 

美国源特级胎牛血清,照射,热灭活品牌代理商

上海金畔生物科技有限公司是美国源特级胎牛血清,照射,热灭活品牌代理商 ,欢迎访问官网了解更多产品信息和订购。
美国源特级胎牛血清,照射,热灭活

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美国源特级胎牛血清,照射,热灭活

产品名称:HyClone Characterized FBS, US Origin, 1000 mL, Irradiated 25-40kGy, Heat-inactivated;美国源特级胎牛血清,照射,热灭活

产品货号:SH30071.04IH25-40;

处理方式:3×100 nm滤膜过滤;

地区来源:美国;

物种来源:;

内毒素水平:≤ 25 EU/mL;

血红素水平:< 25 mg/dL;

热灭活:灭活;

射线处理:处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:Enhance growth of cells with U.S. sourced HyClone Characterized Fetal Bovine Serum (FBS).


HyClone characterized fetal bovine serum (FBS) is sourced in the USA. Complete traceability is provided back to the original source, as certified by the International Serum Industry Association (ISIA).

Additional traceability testing of premium HyClone fetal bovine serum is performed by our collaborator, Oritain.

HyClone FBS serum is low in antibodies and high in growth factors to support robust growth and division of cells, including embryonic stem cells.

HyClone characterized fetal bovine serum (FBS) is produced using true pool technology, which minimizes bottle-to-bottle variability. This FBS product is filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Each lot of FBS is subjected to virus panel testing according to 9 CFR 113.53. HyClone characterized FBS is assayed for many components, including gamma globulin, glucose, and IgG.


Additional treatments are available: HyClone FBS heat inactivated (HI) and HyClone FBS irradiated (IR). Additional testing, including EMEA-conforming testing, is also available.


Fetal bovine serum used in cell culture

Fetal bovine serum (FBS) is frequently added to cell culture media to promote cell growth and division. Because of its high content of growth factors and other nutrients that meet the metabolic needs of eukaryotic cells, FBS serum is frequently used to grow mammalian cells including embryonic stem cells.


HyClone FBS is certified for traceability

The manufacturing processes used to create HyClone serum are traceability certified by the International Serum Industry Association (ISIA). To further support product traceability, we were an early adopter of Scientific Traceability by Oritain to prove the country of origin of premium bovine sera products from the United States, Australia, and New Zealand. Premium bovine serum samples from different regions have been analyzed, and Oritain has created a database of the unique 'Origin Fingerprint' exclusive to each country of origin.


Have Confidence in Security of Supply with Secondary Sourcing

Fetal Bovine Serum from a single origin may be limited, so it’s important to qualify FBS products from other countries to increase your security of supply. HyClone Characterized FBS from United States (US), New Zealand (NZ) and Australia (AUS) have been evaluated and tested to show equivalent performance. 

HyClone特级胎牛血清,照射,热灭活品牌代理商

上海金畔生物科技有限公司是HyClone特级胎牛血清,照射,热灭活品牌代理商 ,欢迎访问官网了解更多产品信息和订购。
HyClone特级胎牛血清,照射,热灭活

详情介绍

HyClone特级胎牛血清,照射,热灭活

产品名称:HyClone Characterized FBS, NZ Origin, 500 mL, Irradiated 25-40kGy, Heat-inactivated;HyClone特级胎牛血清,照射,热灭活

产品货号:SH30406.02IH25-40;

处理方式:3×100 nm滤膜过滤;

地区来源:新西兰;

物种来源:;

内毒素水平:≤ 20 EU/mL;

血红素水平:< 25mg/dL;

热灭活:灭活;

射线处理:处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:Enhance growth of cells with New Zealand sourced HyClone Characterized Fetal Bovine Serum.


Sourced in New Zealand with complete traceability back to the original source.

Low in antibodies and high in growth factors.

Produced using true pool technology for uniformity and consistency from bottle to bottle.

Filtered through three sequential 100 nm (0.1 µm) pore size-rated filters.

Virus panel testing according to 9 CFR 113.53.

HyClone Characterized Fetal Bovine Serum (FBS), New Zealand Origin is carefully collected, processed, and filtered in our own New Zealand facility. The great expanse of the Pacific ocean has protected New Zealand’s islands from many outside influences, both geographically and biologically. One of the benefits of this isolation is that New Zealand has the fewest reported bovine diseases in the world, making Characterized FBS, New Zealand Origin an excellent source of bovine serum as it offers greater security.


Characterized FBS is assayed for gamma globulin, alkaline phosphatase, lactate dehydrogenase, glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, pH, total protein, albumin, blood urea nitrogen, creatinine, total bilirubin, sodium, potassium, calcium, chloride, inorganic phosphorus, osmolality, iron, total iron binding capacity (TIBC), percent iron saturation, glucose, and IgG. Included assays are subject to change without notice.


Additional treatments available including heat-inactivation and irradiation. Additional testing, including EMEA-conforming testing, is also available.


For decades, Cytiva's HyClone™ premium bovine serum products have exceeded industry standards for quality, purity, and regulatory compliance. HyClone™ serum processes are traceability certified by the International Serum Industry Association (ISIA) and, in addition, Cytiva has chosen to be an early adopter of scientific traceability by Oritain to prove the country of origin of their premium bovine serum products from the United States, Australia and New Zealand. 

HyClone澳洲特级胎牛血清 照射,热灭活

上海金畔生物科技有限公司提供HyClone澳洲特级胎牛血清 照射,热灭活 ,欢迎访问官网了解更多产品信息和订购。
HyClone澳洲特级胎牛血清 照射,热灭活

详情介绍

HyClone澳洲特级胎牛血清 照射,热灭活

产品名称:HyClone Characterized FBS, AUS Origin, Processed in NZ, 500 mL, Irradiated 25-40kGy, Heat-Inactivated;HyClone澳洲特级胎牛血清 照射,热灭活

产品货号:SH30084.03IH25-40;

处理方式:3×100 nm滤膜过滤;

地区来源:澳大利亚;

物种来源:;

内毒素水平:内毒素水平:≤ 25 EU/mL;

血红素水平:< 25 mg/dL

热灭活:灭活;

射线处理:处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:This premium HyCloneTM Fetal Bovine Australian-origin Serum is well-characterized, triple 100 nm filtered, virus-tested to 9 CFR standard, and traceability-certified (ISIA, Oritain).


Australian origin: benefit from Australian husbandry methods and certified traceability.

Characterized: assayed for a variety of critical components and biochemical characteristics.

Endotoxin-tested: certificate of analysis confirms ≤ 25 EU/mL endotoxin.

Triple-filtered: serial 100 nm pore size-rated filtering for reliable microbial depletion.

Post-filtration treatments: γ-irradiated and heat-inactivated FBS options also available.

Virus-tested: broad virus panel testing in accordance with 9 CFR 113.53.

Fetal bovine serum (FBS) is a nutrient-rich cell culture supplement that is high in growth factors and low in antibodies, compared to non-fetal sera.


Why choose HyCloneTM Australian Origin FBS?

HyClone Australian Origin serum is sourced from Australian abattoirs approved by the U.S. Department of Agriculture (USDA) or Australian Quarantine and Inspection Service (AQIS). The Australian methods of animal husbandry exhibit excellent animal nutrition and healthcare. Australia is an isolated continent, thus making animal disease and control management easier than in most areas of the world.


Characterized FBS for cell culture

HyClone Characterized FBS is an excellent alternative to more expensive defined sera, and meets the requirements of most discriminating FBS users. The final serum is assayed for important proteins, hormones, growth factors, metabolites, and nutrients that support healthy cell cultures. Assay results are included in the certificate of analysis and biochemical assay list. Additional testing, including EMEA-conforming testing, is also available.


Have Confidence in Security of Supply with Secondary Sourcing

Fetal Bovine Serum from a single origin may be limited, so it’s important to qualify FBS products from other countries to increase your security of supply. HyClone Characterized FBS from United States (US), New Zealand (NZ) and Australia (AUS) have been evaluated and tested to show equivalent performance. Read our full study.


Bacterial endotoxin-tested

Endotoxin contamination is a significant concern in research and manufacturing, especially when producing cell culture-derived products for use in humans or animals. The presence of endotoxin in cell cultures can also have adverse and unpredictable effects on cell growth, function and phenotype that can compromise research results. Our patented closed-system blood collection method and rigorous quality control testing ensure that HyClone Characterized FBS meets industry standards of ≤ 25 EU/mL endotoxin. The final product also contains ≤ 25 mg/dL hemoglobin, which is an indicator of appropriate handling during collection and transport.


Virus-tested and quality-assured for reduced contamination risk

HyClone Characterized FBS is triple-filtered through serial 100 nm (0.10 μm) pore size-rated filters, which has become an industry standard for cell culture grade FBS. Before dispensing, each lot of serum is pooled using true pool technology to minimize variability from bottle to bottle.


The final serum is virus-tested according to 9 CFR 113.53 to demonstrate that there are no detectable levels of specified viruses, pathogenic agents, and hemadsorbing agents. Robust sterility testing further confirms that the final product contains no detectable growth of bacteria or fungi (current USP) and no detectable mycoplasma.


Post-filtration treatments for added process security

Gamma-irradiated and heat-inactivated FBS options are available to further reduce the risk of adventitious agents, particularly when manufacturing products for use in humans or animals. The irradiation process is validated to deliver a minimum dose of 25 kGy.


Regulatory and supply chain security

For decades, Cytiva's HyClone™ premium bovine serum products have exceeded industry standards for quality, purity, and regulatory compliance. HyClone™ serum processes are traceability traceability-certified by the International Serum Industry Association (ISIA) and, . Iin addition, Cytiva has chosen to be an early adopter of scientific traceability by Oritain to prove the country of origin of their premium bovine serum products from the United States, Australia and New Zealand. Premium bovine serum samples from different regions have been analyzed and Oritain has created a database of the unique ‘Origin Fingerprint’ that is exclusive to each country of origin.

射线处理,热灭活,新生牛血清

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射线处理,热灭活,新生牛血清

详情介绍

射线处理,热灭活,新生牛血清

产品名称: HyClone Newborn Bovine Calf Serum, NZ Origin, 500 mL, Irradiated 25-40kGy, Heat-inactivated;射线处理,热灭活,新生牛血清

产品货号:SH30401.01IH25-40;

处理方式:3×100 nm滤膜过滤;

地区来源:新西兰;

物种来源:出生天数小于10天的新生牛;

内毒素水平:内毒素水平:≤ 25 EU/mL;

血红素水平:< 30 mg/dL

热灭活:灭活;

射线处理:处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:A cost-effective alternative to Fetal Bovine Serum (FBS).


Sourced from New Zealand with complete traceability back to original source.

Age of animal at time of collection less than 10 days old.

Low in antibodies and high in growth factors.

Virus panel testing according to 9 CFR 113.53.

All New Zealand calf serum products are carefully collected, processed, and filtered in New Zealand to ensure safety against bovine diseases. We follow the same standards in processing of our calf serum as with our Fetal Bovine Serum (FBS) to enable offering the highest quality and traceability.


New Zealand Newborn Bovine Calf Serum is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

HyClone代理:热灭活补铁型小牛血清(美国产)

上海金畔生物科技有限公司提供HyClone代理:热灭活补铁型小牛血清(美国产) ,欢迎访问官网了解更多产品信息和订购。
HyClone代理:热灭活补铁型小牛血清(美国产)

详情介绍

HyClone代理:热灭活补铁型小牛血清(美国产)

产品名称:HyClone Iron-Supplemented Calf Serum (BCSS), US Origin, 100 mL, Heat-inactivated;HyClone代理:热灭活补铁型小牛血清(美国产)

产品货号:SH30072.02HI;

处理方式:3×100 nm滤膜过滤;

地区来源:美国;

物种来源:16-24周小牛

内毒素水平:内毒素水平:≤ 10 EU/mL

血红素水平:< 30 mg/dL

热灭活:灭活;

射线处理:未处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:Contains additional iron to boost serum transferrin to physiological levels.


Sourced in the USA with complete traceability back to original source.

Cost-effective alternative to FBS.

Age of animal at time of collection approximately 16-24 weeks.

Low in antibodies and high in growth factors.

Virus panel testing according to 9 CFR 113.53.

Supplemented with iron and growth-promoting factors.

HyClone Iron-Supplemented Bovine Calf Serum, U.S. origin is collected by venipuncture from formula-fed veal calves, which produce exceptionally high levels of transferrin. This serum is carefully supplemented with iron to load serum transferrin levels to physiological levels. Bovine calf serum products , when supplemented, can provide three to four times as much available iron as FBS. In many applications, the performance of calf serum will equal or even surpass that of FBS.


Iron-supplemented Bovine Calf Serum, U.S. origin is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

HyClone小牛血清(美国),热灭活

上海金畔生物科技有限公司提供HyClone小牛血清(美国),热灭活 ,欢迎访问官网了解更多产品信息和订购。
HyClone小牛血清(美国),热灭活

详情介绍

HyClone小牛血清(美国),热灭活

产品名称:HyClone Cosmic Calf Serum, US Origin, 500 mL, Heat-inactivated;HyClone小牛血清(美国),热灭活

产品货号:SH30087.03HI;

处理方式:3×100 nm滤膜过滤;

地区来源:美国;

物种来源:16-24周小牛

内毒素水平:内毒素水平:≤ 10 EU/mL

血红素水平:< 30 mg/dL

热灭活:灭活;

射线处理:未处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:A bovine calf serum fortified with iron and growth promoting factors for growing a broad spectrum of cell types.


Sourced in the USA with complete traceability back to original source.

Age of animal at time of collection approximately 16-24 weeks.

Low in antibodies and high in growth factors.

Virus panel testing according to 9 CFR 113.53.

Supplemented with iron and growth-promoting factors.

HyClone Cosmic Calf serum is a high-performing bovine calf serum that has been fortified with iron and a proprietary blend of growth-promoting factors. The result is a naturally derived, high-quality engineered serum that has demonstrated excellent performance with a broad range of cell types.


Cosmic Calf Serum, U.S. origin is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

热灭活美国小牛血清

上海金畔生物科技有限公司提供热灭活美国小牛血清 ,欢迎访问官网了解更多产品信息和订购。
热灭活美国小牛血清

详情介绍

热灭活美国小牛血清

产品名称:HyClone Cosmic Calf Serum, US Origin, 100 mL, Heat-inactivated;

产品货号:SH30087.02HI;热灭活美国小牛血清

处理方式:3×100 nm滤膜过滤;

地区来源:美国;

物种来源:16-24周小牛

内毒素水平:内毒素水平:≤ 10 EU/mL

血红素水平:< 30 mg/dL

热灭活:灭活;

射线处理:未处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:A bovine calf serum fortified with iron and growth promoting factors for growing a broad spectrum of cell types.


Sourced in the USA with complete traceability back to original source.

Age of animal at time of collection approximately 16-24 weeks.

Low in antibodies and high in growth factors.

Virus panel testing according to 9 CFR 113.53.

Supplemented with iron and growth-promoting factors.

HyClone Cosmic Calf serum is a high-performing bovine calf serum that has been fortified with iron and a proprietary blend of growth-promoting factors. The result is a naturally derived, high-quality engineered serum that has demonstrated excellent performance with a broad range of cell types.


Cosmic Calf Serum, U.S. origin is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

Hyclone新西兰小牛血清,热灭活

上海金畔生物科技有限公司提供Hyclone新西兰小牛血清,热灭活 ,欢迎访问官网了解更多产品信息和订购。
Hyclone新西兰小牛血清,热灭活

详情介绍

Hyclone新西兰小牛血清,热灭活

产品名称:HyClone Cosmic Calf, NZ Origin, 1000 mL, Heat-inactivated;Hyclone新西兰小牛血清,热灭活

产品货号:SH30413.03HI;

处理方式:3×100 nm滤膜过滤;

地区来源:新西兰;

物种来源:出生天生小于10天的新生牛

内毒素水平:内毒素水平:≤ 25 EU/mL

血红素水平:< 30 mg/dL

热灭活:灭活;

射线处理:未处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:A bovine calf serum fortified with iron and growth promoting factors for growing a broad spectrum of cell types.


Sourced in New Zealand with complete traceability back to original source.

Age of animal at time of collection less than 10 days old.

Low in antibodies and high in growth factors.

Virus panel testing according to 9 CFR 113.53.

All New Zealand calf serum products are carefully collected, processed, and filtered in New Zealand to ensure safety against bovine diseases. We follow the same standards in processing of our calf serum as with our Fetal Bovine Serum (FBS) to offer the highest quality and traceability.


Cosmic Calf Serum, New Zealand origin is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.

HyClone美国小牛血清,热灭活

上海金畔生物科技有限公司提供HyClone美国小牛血清,热灭活 ,欢迎访问官网了解更多产品信息和订购。
HyClone美国小牛血清,热灭活

详情介绍

HyClone美国小牛血清,热灭活

产品名称:HyClone Bovine Calf Serum (BCS), US Origin, 1000 mL, Heat-inactivated;HyClone美国小牛血清,热灭活

产品货号:SH30073.04HI;

处理方式:3×100 nm滤膜过滤;

地区来源:美国;

物种来源:16-22周小牛;

内毒素水平:内毒素水平:≤ 10 EU/mL

血红素水平:< 30 mg/dL

热灭活:灭活;

射线处理:未处理;

保存条件:≤-10℃;

运输方式:干冰运输;

详细介绍:A cost-effective alternative to Fetal Bovine Serum (FBS).


Sourced in the USA with complete traceability back to original source

Cost-effective alternative to FBS

Age of animal at time of collection approximately 16-24 weeks

Low in antibodies and high in growth factors

Virus panel testing according to 9 CFR 113.53

HyClone Bovine Calf Serum, U.S. origin is collected by venipuncture from formula-fed veal calves, which produce exceptionally high levels of transferrin.

Bovine Calf Serum, U.S. origin is filtered through sequential 100 nm (0.1 µm) pore size-rated filters. Before dispensing, each lot of serum is pooled using true pool technology to ensure uniformity and consistency between bottles.