上海金畔生物科技有限公司提供Gibco 预装Sf-900 II 培养基的Wave Cellbag ，欢迎访问官网了解更多产品信息和订购。
Gibco 预装Sf-900 II 培养基的Wave Cellbag
Gibco™ Wave Cellbag prefilled with Sf-900™ II Medium
Product Type:Cellbag Single-Use Bioreactor.
Shipping Condition:Room Temperature.
Product Line:Gibco™, SynerGy™.
Form:Liquid.
Glutamine:L-Glutamine.
Cell Type:Insect Cells.
Cell Line:Sf21, Sf9.
Classification:Protein-Free, Serum-Free.
Serum Level:Serum-Free.
上海金畔生物科技有限公司提供Gibco™ Sf-900™ II 无血清培养基 ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Sf-900™ II 无血清培养基
Gibco™ Sf-900™ II SFM（Gibco™ Sf-900™ II 无血清培养基）
This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900™ II SFM features:
• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors
Superior long-term, high-density growth:
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900™ II SFM is capable of supporting the cultures to >20 passages.
Optimized for recombinant protein production:
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.
Serum-free, protein-free, ready-to-use formulation:
Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.
Scalable in bioreactors:
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).
Product use:
Customers using Gibco™ Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP manufacturing and quality system:
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
上海金畔生物科技有限公司提供Gibco™ Sf-900™ II SFM, no methionine, no cystine ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Sf-900™ II SFM, no methionine, no cystine
Gibco™ Sf-900™ II SFM, no methionine, no cystine
This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Gibco™ Sf-900™ II SFM features:
• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors
Superior long-term, high-density growth:
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 × 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Gibco™ Sf-900™ II SFM is capable of supporting the cultures to >20 passages.
Optimized for recombinant protein production:
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.
Serum-free, protein-free, ready-to-use formulation:
Gibco™ Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.
Scalable in bioreactors:
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).
Product use:
Customers using Gibco™ Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP manufacturing and quality system:
For supply chain continuity, we manufacture Gibco™ Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
上海金畔生物科技有限公司提供SIM SF 培养基 ，欢迎访问官网了解更多产品信息和订购。
SIM SF 培养基
培养基特点：
SIM SF 培养基为澄清淡黄色透明液体，如果一旦发现培养基变得浑浊或出现沉淀，请勿使用。
保存条件：
2~8℃避光保存，一旦过期，请勿使用。
稳定性及保质期：
室温下一个星期内稳定。
自收到之日起， 2~8℃避光保存，六个月内稳定。
细胞冻存：
选择处于对数生长期的细胞，保证活率大于90%。
制备细胞冻存液：45%新鲜培养基，45%条件培养基，10%DMSO。
200g离心5分钟，弃去上清。
用细胞冻存液重悬细胞，细胞冻存密度为1.2~2×107/ml/ml。
迅速转移重悬的细胞至无菌的冻存管中，每管1~2ml。
将冻存管放入程序降温盒中，-70℃冰箱放置过夜。
若冻存细胞需要长期保存，将其转移至液氮罐中。
细胞复苏：
将冻存管迅速转移至27℃水浴中，进行融化。
将融化的细胞转至无菌的含5-10ml新鲜培养基的无菌离心管中。
200g低速离心5分钟，缓慢倒掉上部培养基，不要将沉降的细胞悬起。
用10-20ml新鲜培养基重悬细胞，确保重悬后的细胞密度大于1×106/ml左右。
将重悬的细胞放入无菌培养瓶中，旋盖拧松，放在摇床中悬浮培养。
当细胞密度生长达到2~4×106/ml时，可以进行传代，传代时细胞接种密度为0.8-1×106/ml。
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
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Proteinase K, SF488 Labeled
Similar products: Alexa Fluor 488 Proteinase K, FITC Proteinase K.
Product Description:
SF488 labeled Proteinase K is one of Nanocs’ fluorescent Proteinase K conjugates. Proteinase K is a highly reactive nonspecific serine protease that is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9. Nanocs provides a number of fluorescent labeled Proteinase K products that span from UV region to near IR. These fluorescent Proteinase K conjugates can be used for enzyme studies, bioassay development and many other biomedical research. Nanocs SF488 labeled Proteinase K is a green fluorescent Proteinase K conjugate that has excitation/emission wavelength at 495/515 nm. This SF488 Proteinase K can be easily detected by fluorescent instruments in FITC channel. All our fluorescent Proteinase K conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
Product Specifications:
Storage Conditions:
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
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Proteinase K, SF546 Labeled
Similar products: Alexa Fluor 546 Proteinase K, Cy3 Proteinase K.
Product Description:
SF546 labeled Proteinase K is one of Nanocs’ fluorescent Proteinase K conjugates. Proteinase K is a highly reactive nonspecific serine protease that is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9. Nanocs provides a number of fluorescent labeled Proteinase K products that span from UV region to near IR. These fluorescent Proteinase K conjugates can be used for enzyme studies, bioassay development and many other biomedical research. Nanocs SF546 labeled Proteinase K is a red fluorescent Proteinase K conjugate that has excitation/emission wavelength at 546/570 nm. This red Proteinase K can be easily detected by fluorescent instruments in Cy3 channel. All our fluorescent Proteinase K conjugates were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
Product Specifications:
Storage Conditions:
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
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Bead Properties:
Storage Conditions:
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Bead Properties:
Storage Conditions:
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Bead Properties:
Storage Conditions:
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Description:
SFTM488 fluorophores aim to providing better fluorescent molecular tools for our customers. Besides their superior fluorescent properties, e.g. high brightness, super photo-stability, these fluorophores are designed to simplify your fluorescent labeling and detection process. SFTM488 fluorophores can be used directly in your aqueous working buffer without need adding any organic solvents. Elimination of organic solvents not only simplifies your labeling and detection process, it also provides additional protection of your molecules from damaging caused by some harsh organic solvents. In addition, SFTM fluorophores offer diversified chemically reactive and/or biologically active moieties that can be used directly for labeling, imaging and detecting of various molecules and particles. These fluorophores range from UV to near IR region and can be used both in-vitro and in-vivo imaging. In short, Nanocs SFTM fluorophores offer following advantages:
Specifications:
Storage:
Store at -20 0C. Protect from light.
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Description:
SFTM fluorophores aim to providing better fluorescent molecular tools for our customers. Besides their superior fluorescent properties, e.g. high brightness, super photo-stability, these fluorophores are designed to simplify your fluorescent labeling and detection process. SFTM fluorophores can be used directly in your aqueous working buffer without need adding any organic solvents. Elimination of organic solvents not only simplifies your labeling and detection process, it also provides additional protection of your molecules from damaging caused by some harsh organic solvents. In addition, SFTM fluorophores offer diversified chemically reactive and/or biologically active moieties that can be used directly for labeling, imaging and detecting of various molecules and particles. These fluorophores range from UV to near IR region and can be used both in-vitro and in-vivo imaging. In short, Nanocs SFTM fluorophores offer following advantages:
Specifications:
Storage:
Store at -20 0C.
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Description:
SFTM fluorophores aim to providing better fluorescent molecular tools for our customers. Besides their superior fluorescent properties, e.g. high brightness, super photo-stability, these fluorophores are designed to simplify your fluorescent labeling and detection process. SFTM fluorophores can be used directly in your aqueous working buffer without need adding any organic solvents. Elimination of organic solvents not only simplifies your labeling and detection process, it also provides additional protection of your molecules from damaging caused by some harsh organic solvents. In addition, SFTM fluorophores offer diversified chemically reactive and/or biologically active moieties that can be used directly for labeling, imaging and detecting of various molecules and particles. These fluorophores range from UV to near IR region and can be used both in-vitro and in-vivo imaging. In short, Nanocs SFTM fluorophores offer following advantages:
Specifications:
Storage:
Store at -20 0C.
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Description:
SFTM fluorophores aim to providing better fluorescent molecular tools for our customers. Besides their superior fluorescent properties, e.g. high brightness, super photo-stability, these fluorophores are designed to simplify your fluorescent labeling and detection process. SFTM fluorophores can be used directly in your aqueous working buffer without need adding any organic solvents. Elimination of organic solvents not only simplifies your labeling and detection process, it also provides additional protection of your molecules from damaging caused by some harsh organic solvents. In addition, SFTM fluorophores offer diversified chemically reactive and/or biologically active moieties that can be used directly for labeling, imaging and detecting of various molecules and particles. These fluorophores range from UV to near IR region and can be used both in-vitro and in-vivo imaging. In short, Nanocs SFTM fluorophores offer following advantages:
Specifications:
Storage:
Store at -20 0C.
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Description:
SFTM fluorophores aim to providing better fluorescent molecular tools for our customers. Besides their superior fluorescent properties, e.g. high brightness, super photo-stability, these fluorophores are designed to simplify your fluorescent labeling and detection process. SFTM fluorophores can be used directly in your aqueous working buffer without need adding any organic solvents. Elimination of organic solvents not only simplifies your labeling and detection process, it also provides additional protection of your molecules from damaging caused by some harsh organic solvents. In addition, SFTM fluorophores offer diversified chemically reactive and/or biologically active moieties that can be used directly for labeling, imaging and detecting of various molecules and particles. These fluorophores range from UV to near IR region and can be used both in-vitro and in-vivo imaging. In short, Nanocs SFTM fluorophores offer following advantages:
Specifications:
Storage:
Store at -20 0C.
上海金畔生物科技有限公司代理日本三博特sanplatec全线产品，欢迎访问官网了解更多产品信息。
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产品名称：
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FEP螺旋管（称呼：SF-14、外径×内径（φmm）：14×12）
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| 产品编号： | WEB23778 | ||
| 价格： | 会员价：0元；市场价：0元 | ||
| 产品特点： |
结束工序简单进行，可从任意一边拉出管或线。
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| 产品规格： | |||
| 材料： |
确认材料的耐药性 >> 耐药性检索 |
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产品特征：
上海金畔生物科技有限公司代理日本三博特sanplatec全线产品，欢迎访问官网了解更多产品信息。
|
产品名称：
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FEP螺旋管（称呼：SF-10、外径×内径（φmm）：10.6×9）
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||
| 产品编号： | WEB23777 | ||
| 价格： | 会员价：0元；市场价：0元 | ||
| 产品特点： |
结束工序简单进行，可从任意一边拉出管或线。
|
||
| 产品规格： | |||
| 材料： |
确认材料的耐药性 >> 耐药性检索 |
||
产品特征：,
上海金畔生物科技有限公司代理日本三博特sanplatec全线产品，欢迎访问官网了解更多产品信息。
|
产品名称：
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FEP螺旋管（称呼：SF-08、外径×内径（φmm）：7.5×6）
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| 产品编号： | WEB23776 | ||
| 价格： | 会员价：0元；市场价：0元 | ||
| 产品特点： |
结束工序简单进行，可从任意一边拉出管或线。
|
||
| 产品规格： | |||
| 材料： |
确认材料的耐药性 >> 耐药性检索 |
||
产品特征：
上海金畔生物科技有限公司代理日本三博特sanplatec全线产品，欢迎访问官网了解更多产品信息。
|
产品名称：
|
FEP螺旋管（称呼：SF-06、外径×内径（φmm）：5.5×4）
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||
| 产品编号： | WEB23775 | ||
| 价格： | 会员价：0元；市场价：0元 | ||
| 产品特点： |
结束工序简单进行，可从任意一边拉出管或线。
|
||
| 产品规格： | |||
| 材料： |
确认材料的耐药性 >> 耐药性检索 |
||
产品特征：,
上海金畔生物科技有限公司代理日本三博特sanplatec全线产品，欢迎访问官网了解更多产品信息。
|
产品名称：
|
FEP螺旋管（称呼：SF-04、外径×内径（φmm）：3.6×2.6）
|
||
| 产品编号： | WEB23774 | ||
| 价格： | 会员价：0元；市场价：0元 | ||
| 产品特点： |
结束工序简单进行，可从任意一边拉出管或线。
|
||
| 产品规格： | |||
| 材料： |
确认材料的耐药性 >> 耐药性检索 |
||
产品特征：
上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。
|
Description:
SFTM fluorophores aim to providing better fluorescent molecular tools for our customers. Besides their superior fluorescent properties, e.g. high brightness, super photo-stability, these fluorophores are designed to simplify your fluorescent labeling and detection process. SFTM fluorophores can be used directly in your aqueous working buffer without need adding any organic solvents. Elimination of organic solvents not only simplifies your labeling and detection process, it also provides additional protection of your molecules from damaging caused by some harsh organic solvents. In addition, SFTM fluorophores offer diversified chemically reactive and/or biologically active moieties that can be used directly for labeling, imaging and detecting of various molecules and particles. These fluorophores range from UV to near IR region and can be used both in-vitro and in-vivo imaging. In short, Nanocs SFTM fluorophores offer following advantages:
Specifications:
Storage:
Store at -20 0C.
