大鼠食管上皮细胞

上海金畔生物科技有限公司提供大鼠食管上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
大鼠食管上皮细胞

详情介绍

product name:Rat Esophageal Epithelial Cells(大鼠食管上皮细胞);

cat no:ABC-TC4133;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Rat Primary Esophageal Epithelial Cells(大鼠食管上皮细胞)from AcceGen are isolated from tissue of adult laboratory Sprague–Dawley Rats. Rat Primary Esophageal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1×10^6 cells and is delivered frozen.

Rat Primary Esophageal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Esophageal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.

Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

KYSE30人食管鳞癌细胞

上海金畔生物科技有限公司提供KYSE30人食管鳞癌细胞 ,欢迎访问官网了解更多产品信息和订购。
KYSE30人食管鳞癌细胞

详情介绍
Product Name

KYSE30人食管鳞癌细胞

Cat.No

ABC-TC487S

Species

Human

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Tissue: oesophagus; Tumor: carcinoma, squamous cell. Karotype: aneuploid. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B have been reported. The cells express a large amount of epidermal growth factor receptors. Passage number: 340.

KYSE30人食管鳞癌细胞

组织:食道;肿瘤:癌,鳞状细胞。核型:非整倍体。已经报道了内含子 6 剪接受体位点的 p53 突变和 c-erb B 的 12 倍扩增。细胞表达大量表皮生长因子受体。段落编号:340。

Growth Properties

Polygonal

Recommended Medium And Supplement

RPMI 1640 + Ham’s F12 (1: 1) + 2% FBS + 2 mM L-Glutamine; Split confluent cultures 1: 5-1: 10 using trypsin/EDTA; seed at 1×104 cells/cm2; 37℃, 5% CO2.

Application

For research use only

Product Type

Human Esophageal Cancer Cell Lines

Product Image

北京上海金畔代理accegen在中国的业务。
上海金畔生物科技有限公司
一、辐射五大服务方向:
国内试剂耗材经销代理;国外试剂订购(直订或境外代理);基因组测序(二代、二代+三代);基因分型(SNP检测):低//高通量项目;生物样本运输;
二、多家代理品牌,涉及30多种,经营代理500多个品牌:
accegenIntact GenomicSigmaEpigentekThermoFisherDNA GenotekUSA Scientific……
所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。
专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作