中科百抗RPMI1640培养基的组分和作用

中科百抗RPMI1640培养基的组分和作用

RPMI 1640最初是为淋巴细胞培养专门设计的,目前已广泛应用于各种正常细胞和癌细胞的培养,尤其是悬浮细胞的培养,是使用最为广泛的培养基之一。像K-562HL-60JurkatDaudiIM-9等成淋巴细胞、T细胞淋巴瘤细胞以及HCT-15上皮细胞等均可参考使用。那么你知道中科百抗RPMI1640培养基的组分和作用是什么吗?让我们一起来看看吧!

一、L-谷氨酰胺

L-谷氨酰胺在细胞培养过程中扮演着至关重要的角色。在脱去氨基之后,L-谷氨酰胺成为培养细胞所需能量的来源,同时也参与蛋白质的构建和核酸代谢。在溶液中,L-谷氨酰胺经过一段时间会发生分解,然而其具体的分解速率尚未得到最终确定。L-谷氨酰胺的降解会产生氨,而氨对某些细胞来说具有有毒性。

二、GlutaMAX-I

L-谷氨酰胺的衍生物,可替代细胞培养基中等摩尔的L-谷氨酰胺。该物质在水溶液中十分稳定,不会自发降解,而是通过细胞分泌的一种肽酶缓慢降解以释放谷氨酰胺,再被细胞吸收利用。

三、HEPES

HEPES是一种zhuo越的生物缓冲剂,对细胞毫无毒性之作用,加入HEPES的培养基可持久地保持恒定的pH范围,从而有效地抵御培养液pH波动对细胞生长所造成的不良影响。在进行克隆化培养时,一般会投入HEPES

四、丙酮酸钠

丙酮酸钠可作为细胞培养中替代碳源之用。尽管细胞更偏好以葡萄糖为碳源,但若无葡萄糖,则细胞亦能代谢丙酮酸钠。

中科百抗RPMI1640 培养基产品,采用注射级药品原料药,在药品GMP车间生产,具备ji的质量保障,ji的无菌保障,极低的细菌内毒素,极低的重金属杂质含量。

更多有关中科百抗RPMI1640培养基的组分和作用,请联系上海金畔生物科技有限公司。

RPMI 1640培养基与DMEM培养基有什么区别?

RPMI 1640培养基与DMEM培养基有什么区别?

细胞培养基是一种提供细胞生长所需的营养物质和环境条件的液体培养介质。它模拟了细胞在体内的生长环境,为细胞提供必要的营养和支持,以保持其生存和增殖。那么RPMI 1640培养基与DMEM培养基有什么区别?

首先,我们来了解一下RPMI 1640培养基。RPMI 1640培养基是一种富含氨基酸和维生素的培养基,适用于多种细胞类型的培养。它的主要组成包括氨基酸、葡萄糖、维生素、盐类和缓冲剂等。与此相比,DMEM培养基的成分更复杂,除了氨基酸、葡萄糖和维生素外,还含有有机酸、胆红素、胆碱和核酸等物质。
就营养物质含量而言,RPMI 1640培养基中的葡萄糖浓度较低(通常为2g/L),适合代谢较慢的细胞。相比之下,DMEM培养基中的葡萄糖浓度较高(通常为4.5g/L),适合代谢较快的细胞。此外,RPMI 1640培养基中的氨基酸浓度通常较高,而DMEM培养基则相对较低。
pH值和缓冲剂也是RPMI 1640培养基和DMEM培养基之间的区别之一。RPMI 1640培养基的pH值一般为7.4,可以通过添加HEPES等缓冲剂来调节。DMEM培养基的pH值也为7.4,但通常使用碳酸氢盐缓冲液进行调节。
在应用范围上,RPMI 1640培养基主要适用于培养代谢速率较慢的细胞类型,如淋巴细胞、白血病细胞和肿瘤细胞。它在免疫学、细胞毒性测试和淋巴细胞增殖等方面有广泛的应用。而DMEM培养基则更适合培养代谢较快的细胞类型,如肿瘤细胞、上皮细胞和成纤维细胞。它在细胞生物学、分子生物学、病毒培养和基因转染等领域被广泛应用。
总结一下,RPMI 1640培养基和DMEM培养基在成分、营养物质含量、pH值和应用范围方面有一些差异。选择适合的培养基要考虑细胞类型、培养条件和实验要求等因素。在使用时,建议参考相关文献或咨询专业人士,以确保获得最佳的培养效果。了解这些差异将有助于科学家和研究人员更好地选择和应用适合自己研究的培养基,上海金畔代理的中科百抗的1640培养基,最近推出了500ml免费试用的福利活动,此培养基含有还原型谷胱甘肽和高浓度的维生素,并且也含有EMEM和DMEM中没有的生物素、维生素B12和对氨基ben甲酸,以及高浓度的氯化胆碱和肌醇,有助于细胞培养。

货号

产品名称

规格

BM32002

中科百抗RPMI 1640培养基

500mL


如果你有更多关于RPMI 1640培养基与DMEM培养基有什么区别的问题,请咨询上海金畔客服!

现货供应:Gibco RPMI 1640培养基(C11875500BT)

现货供应:Gibco RPMI 1640培养基(C11875500BT)

 Gibco1962年成立至今60余年,一直致力于以世界yi流的研发实力、质量体系和强健的供应链赋能生物制药企业的研发和生产。作为细胞培养基的市场ling导者,一直在制定和yin领标准。在生命科学行业中,Gibco在科学出版物中被引用的次数位居前列,深受广大研发者的认可,其培养基质量和一致性经得起长时间和大量试验的考验。

RPMI-1640(Roswell Park Memorial Institute medium 1640)是一种由Roswell Park纪念研究所开发的细胞培养基,适用于淋巴细胞、骨髓细胞、白血病细胞等多种类型的哺乳动物细胞系的培养。RPMI-1640中含有较低浓度的葡萄糖(2000mg/L)、氨基酸、维生素和微量元素等营养物质。此外,RPMI-1640还添加了缓冲剂HEPES和碳酸氢盐,能够保持细胞在较窄的pH范围内正常生长。下面让我们一起来看看Gibco RPMI 1640培养基(C11875500BT)的产品属性吧!

细胞系:HeLaJurkatMCF-7PC-12PBMC、星形胶质细胞和癌细胞

分类:无动物来源

细胞类型:白血病细胞

形式:液体

血清水平:标准血清添加

浓度:1 X

有效期:12个月

添加剂:谷氨酰胺, 酚红

无菌:无菌过滤

不含添加剂:不含 HEPES

运输条件:室温

上海金畔生物科技有限公司现货供应Gibco RPMI 1640培养基(C11875500BT),欢迎选购!

中科百抗RPMI 1640培养基代理商

上海金畔生物科技有限公司是中科百抗RPMI 1640培养基代理商 ,欢迎访问官网了解更多产品信息和订购。
中科百抗RPMI 1640培养基代理

详情介绍

产品简介:

中科百抗RPMI 1640培养基代理,本产品是为淋巴细胞培养专门设计的,目前已广泛应用于各种正常细胞和癌细胞的培养,尤其是悬浮细胞的培养,是使用最为广泛的培养基之一。RPMI 1640培养基含有EMEMDMEM中没有的生物su、维生素B12和对氨基苯甲酸,以及高浓度的氯化dan碱和肌醇。

中科百抗RPMI 1640培养基与其它培养基的区别在于含有还原型gu胱甘肽和高浓度的维生素。RPMI 1640培养基含有EMEMDMEM中没有的生物su、维生素B12和对氨基苯甲酸,以及高浓度的氯化dan碱和肌醇。

本产品含有:D-葡萄糖、酚红、L-gu氨酰胺。不含有:丙酮酸钠、HEPES

产品优势

中科百抗RPMI1640 培养基产品,采用注射级药品原料药,在药品GMP车间生产,具备ji高的质量保障,ji高的无菌保障,极低的细菌内毒素,极低的重金属杂质含量。

产品参数

规格:500mL

性状:本品为浅黄色或无色的澄清液体

pH值:7.0~7.4

渗透压摩尔浓度:260 mOsmol/kg~305 mOsmol/kg

细菌内毒素:<0.25 EU/ml

无菌:应符合规定

细胞生长试验:细胞形态,成纤维样贴壁生长,形态正常无变异细胞数量培养48~72h细胞数量不低于1×105/ml;继续维持培养48h细胞数量不低于2×105/ml

保存温度:2-8℃,避光

效期:自生产之日起12个月

运输条件:室温

产品订购:

货号

产品名称

规格

BM32002

中科百抗RPMI 1640培养基

500mL


更多有关中科百抗RPMI 1640培养基产品介绍,请联系中科百抗RPMI 1640培养基代理——上海金畔生物科技有限公司:

RPMI 1640培养基Gibco

上海金畔生物科技有限公司提供RPMI 1640培养基Gibco ,欢迎访问官网了解更多产品信息和订购。
RPMI 1640培养基Gibco

详情介绍

RPMI 1640 Medium,powder,HEPES(RPMI 1640培养基Gibco)

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.


Product Intended Use:

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).


cGMP Manufacturing and Quality System:

Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ RPMI 1640 product made in our Scotland facility (13018-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Gibco RPMI 1640培养基大量

上海金畔生物科技有限公司提供Gibco RPMI 1640培养基大量 ,欢迎访问官网了解更多产品信息和订购。
Gibco RPMI 1640培养基大量

详情介绍

Gibco RPMI 1640培养基大量

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-059). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no

上海金畔生物科技有限公司提供Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no ,欢迎访问官网了解更多产品信息和订购。
Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no

详情介绍

Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no phenol red

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.


cGMP Manufacturing and Quality System:

SILAC RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco™ RPMI 1640 Medium, HEPES, no glutamine

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, HEPES, no glutamine ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, HEPES, no glutamine

详情介绍

Gibco™ RPMI 1640 Medium, HEPES, no glutamine

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.


cGMP Manufacturing and Quality System:

Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Gibco™ RPMI 1640 Medium

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium

详情介绍

Gibco™ RPMI 1640 Medium

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-158). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ RPMI 1640 Medium, no glucose

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, no glucose ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, no glucose

详情介绍

Gibco™ RPMI 1640 Medium, no glucose

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco™ RPMI 1640 Medium, no phenol red

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, no phenol red ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, no phenol red

详情介绍

Gibco™ RPMI 1640 Medium, no phenol red

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (11835-063). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEP

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEP ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEP

详情介绍

Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Using RPMI 1640 Medium:

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (72400-054). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.


Gibco™ RPMI 1640 Medium (ATCC modification)

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium (ATCC modification) ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium (ATCC modification)

详情介绍

Gibco™ RPMI 1640 Medium (ATCC modification)

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has been modified by ATCC to contain high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, and low L-glutamine. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (1.5 g/L) and therefore requires a 5–7% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco™ RPMI 1640 Medium, powder, HEPES

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, powder, HEPES ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, powder, HEPES

详情介绍

Gibco™ RPMI 1640 Medium, powder, HEPES

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.


Product Intended Use:

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).


cGMP Manufacturing and Quality System:

Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ RPMI 1640 product made in our Scotland facility (13018-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


Gibco™ RPMI 1640 Medium

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium

详情介绍

Gibco™ RPMI 1640 Medium

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-059). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

详情介绍

Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

Using RPMI 1640 Medium:

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (61870-010). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

详情介绍

Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

Using RPMI 1640 Medium:

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (61870-010). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

Gibco™ RPMI 1640 Medium, powder

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, powder ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, powder

详情介绍

Gibco™ RPMI 1640 Medium, powder

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ RPMI 1640 Medium, no glutamine

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, no glutamine ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, no glutamine

详情介绍

Gibco™ RPMI 1640 Medium, no glutamine

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (31870-074). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco™ RPMI 1640 Medium, HEPES

上海金畔生物科技有限公司提供Gibco™ RPMI 1640 Medium, HEPES ,欢迎访问官网了解更多产品信息和订购。
Gibco™ RPMI 1640 Medium, HEPES

详情介绍

Gibco™ RPMI 1640 Medium, HEPES

Using RPMI:

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (52400-025). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.