Gibco™ Human Plasma-Like Medium (HPLM)

上海金畔生物科技有限公司提供Gibco™ Human Plasma-Like Medium (HPLM) ,欢迎访问官网了解更多产品信息和订购。
Gibco™ Human Plasma-Like Medium (HPLM)

详情介绍

Gibco™ Human Plasma-Like Medium (HPLM)

The widely used classic synthetic cell culture media, including MEM, DMEM, RPMI 1640, and DMEM/F-12 contain glucose, amino acids, vitamins, and salts at concentrations that often do not reflect those found in human plasma. These media also lack additional plasma components needed to mimic the metabolic profile of human plasma. When studying cancer and other diseases, results with more physiological relevance will enable researchers to improve their understanding of human function and illness.


The HPLM solution:

HPLM contains more than 60 polar metabolites such as amino acids, nucleic acids, sugars, and small organic acids at concentrations found in human plasma. The salt concentrations in HPLM mimic the salt concentrations found in human plasma. In resembling the natural cellular environment found in the body, HPLM provides researchers with the ability to study the impact of physiologically relevant cell media on their specific applications.


When supplemented with fetal bovine serum (FBS), HPLM is capable of supporting cell growth and viability comparable to those of conventional basal media supplemented with FBS. For most cell lines, adaptation is not required to transition from conventional medium to HPLM.


The complete HPLM formulation is available (hyperlink to technical-resource page with complete HPLM formulation).


cGMP manufacturing and quality system:

HPLM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.



Human RA PB Plasma

上海金畔生物科技有限公司提供Human RA PB Plasma ,欢迎访问官网了解更多产品信息和订购。
Human RA PB Plasma

详情介绍

product name:Human RA PB Plasma

cat no:ABC-TC4053;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Rheumatoid Arthritis (RA) peripheral blood plasma is obtained by centrifugation of the RA PB. This product has a diminished number of cells and platelets. Fresh RA PB plasma is available upon request.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

miRCURY Exosome Serum/Plasma Kit

上海金畔生物科技有限公司提供miRCURY Exosome Serum/Plasma Kit ,欢迎访问官网了解更多产品信息和订购。
miRCURY Exosome Serum/Plasma Kit

详情介绍

Features:

· 外泌体和其他细胞外囊泡恢复良好

· 简单而直接的操作,只需不到2小时

· 不需要超离心或苯酚/氯仿步骤

· *兼容miRCURY LNA miRNA PCR体系

· 适合多种应用,如miRNA或RNA分析

Product Details

miRCURY Exosome Serum/Plasma Kit可实现高质量、可扩展的外泌体分离,操作简单,不需要特殊的实验室设备。miRCURY外泌体血清/血浆试剂盒针对血清和血浆样本进行优化,而miRCURY外泌体细胞/尿液/脑脊液试剂盒用于处理细胞条件培养基、尿液和脑脊液样本。两种试剂盒都提供高的外泌体回收率。

Performance

从血清和血浆中高效分离外泌体

miRCURY外泌体血清/血浆试剂盒用于从血清和血浆样品中分离外泌体。该试剂盒包括从血浆中高效分离外泌体的凝血酶。为了评估分离外泌体的效率,我们定量了外泌体与弃掉上清液的比例。

从细胞条件培养基、尿液和脑脊液中有效分离外泌体

miRCURY外泌体细胞/尿液/脑脊液试剂盒用于从细胞条件培养基、尿液和脑脊液样本中分离外泌体。在比较这些样品类型的微球和上清液中外泌体的比例的研究中,我们观察到外泌体的富集率非常高。

通常,在健康个体中,尿液和脑脊液样本中存在的miRNA数量非常有限。因此,我们强烈建议在RNA分离和PCR分析之前对这些生物体液进行外泌体分离。这使得您可以使用外泌体分离试剂盒作为一种浓缩样品的方法,同时保持潜在的PCR抑制剂的数量降到很低。

Principle

miRCURY Exosome Serum/Plasma Kit利用沉淀富集外泌体和其他细胞外囊泡。它们经过优化,可从各种生物体液样本(如细胞条件培养液、尿液、脑脊液、血清或血浆)中有效分离外泌体。该操作易于使用,只需两小时。

虽然它们不是针对特定物种的,但试剂盒针对人体生物体液进行了优化。

Procedure

样品被离心除去细胞和碎片,以及之前冷冻的样品的低温沉淀。加入沉淀缓冲液,室温孵育1小时。外泌体在30分钟的离心过程中被制成小球。重悬后,外泌体可用于下游分析。

Applications

miRCURY Exosome Serum/Plasma Kit主要用于miRNA/RNA分析应用。