标签归档：glucan

Beta葡聚糖[大麦；中黏度] Beta-Glucan (Barley;Med Viscosity) 5g 货号：P-BGBM Megazyme试剂盒

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Beta葡聚糖[大麦；中黏度]

英文名：Beta-Glucan (Barley;Med Viscosity) 5g

货号：P-BGBM

规格：5 grams

市场价： 5194元

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 1. Which barley beta-glucan do you recommend for the glucanase assay? There are three kinds of barley beta-glucan availabale from Megazyme – high viscosity, medium viscosity and low viscosity.

For glucanase assay, we recommend the medium viscosity beta-glucan.  We now offer low, medium and high viscosity wheat arabinoxylans, and we think that the low viscosity material will be best (easiest) to use in the reducing-sugar assay.

2. Both substrates; barley beta-glucan and wheat arabinoxylan, are standardised to a specific viscosity, e.g. 23-24 cSt. Are the substrates adjusted to give this viscosity?

The substrates are enzymically treated to yield the required viscosity (20 ~ 30 cSt). Modification of the viscosity of beta-glucan is required for IOB viscometric malt beta-glucanase test (which works well).

3. I would like to vacuum dry the standards instead of measuring the moisture content. I wish to dry them at a temperature lower than used in the preparation in Megazyme. Would you please give me some suggestions?

We suggest that you dry the β-glucan powders further in a vacuum oven at about 50˚C.  This will lower the moisture content to approximately 1-2%.

4. I understand a specific polymer can be used for the assay of malt β-glucanase and celluloses. I would very much like to know which polymer this β-glucan is?

The polymer you require is barley beta-glucan (medium viscosity).  The beta-glucan that we supply is pure 1,3:1,4-beta-D-glucan.  Barley β-glucan is also susceptible to hydrolysis by cellulase.

5. Although not specified on the beta-glucan data sheet, is the ratio of 1-3 to 1-4 bonds measured? If so, what is the ratio and would you expect it to remain standardised over a number of different batches?

The content of 1,3 bonds in barley beta-glucan is about 32% (from literature).  We would not expect this to change much (if at all) over different batches.

6. We have measured the molecular weight of beta-glucan originating from barley ordered from your company. Can you please tell us which method have you used for measuring molecular weight?

The MW’s were determined by Multiangle laser light scattering technique.

7. I have purchased barley beta-glucan (lot 30108) and carob galactomannan low viscosity (lot 30702) and would like to know what else there might be in these substrates.

Barley beta-glucan lot 30108 would contain about 3-4% arabinoxylan (this was produced in 1993).  Material supplied post 1995 contains < 0.5% arabinoxylan.  Carob galactomannan is quite pure (> 96%).

8. Low, Medium, High Viscosity β-Glucans, are they naturally occurring at these MW’s or are the MW fractions obtained by some other process?

The high molecular weight beta-glucan is the naturally occurring; this is enzymically modified to give the other viscosities. 

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Besa葡聚糖CFA标准[即以前的FIA标准] ?-Glucan CFA Standard – 4Vials Beta-Glucan CFA Standard – 4Vials 货号：P-BGCFA Megazyme试剂盒

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Besa葡聚糖CFA标准[即以前的FIA标准] ?-Glucan CFA Standard – 4Vials

英文名：Beta-Glucan CFA Standard – 4Vials

货号：P-BGCFA

规格：4 vials

市场价： 2968元

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酵母葡聚糖酶检测试剂盒 Enzymatic Yeast β-Glucan Assay Kit 货号：K-EBHLG Megazyme试剂盒

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酵母葡聚糖酶检测试剂盒

英文名：Enzymatic Yeast β-Glucan Assay Kit

货号：K-EBHLG

规格：40 assays per kit

市场价： 4346元

An enzymatic procedure for the measurement of 1,3:1,6-β-glucan in yeast. Also measures 1,3-β-glucan. Content:40 assays per kit

酵母葡聚糖酶检测试剂盒

UV-method for the determination of Ammonia in foodstuffs,
beverages and other materials

Principle:
(microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+
+ H2O

Kit size:                            96 assays (manual) / 960 (microplate)
                                         / 960 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 3 min
Detection limit:                 0.07 mg/L
Application examples: 
Grape juice, wine, fruit juices, soft drinks, dairy products (e.g. milk),
dietetic food, soy sauce, eggs and egg products, cheese, meat,
processed meat, seafood, bakery products (and baking agents),
fertilisers, pharmaceuticals, tobacco, cosmetics, water, Kjeldahl
analysis, paper (and cardboard), water and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by MEBAK

Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
  • Enzyme supplied as stabilised suspension
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

 

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

Megazyme酵母葡聚糖酶检测试剂盒Enzymatic操作视频（K-EBHLG）

AZCL-Barley β-Glucan (fine) AZCL-Barley β-Glucan (fine) 货号：I-AZBGLF Megazyme试剂盒

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AZCL-Barley β-Glucan (fine)

英文名：AZCL-Barley β-Glucan (fine)

货号：I-AZBGLF

规格：3 grams

市场价： 3100元

 High purity dyed, crosslinked and finely milled insoluble AZCL-Barley β-Glucan for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.

Substrate for the assay of malt β-glucanase, lichenase and cellulases.

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葡聚糖1,4-α-麦芽六糖 Glucan 1,4-α-maltohexaosidase 货号：E-MAL6M Megazyme试剂盒

发表于由
葡聚糖1,4-α-麦芽六糖

英文名：Glucan 1,4-α-maltohexaosidase

货号：E-MAL6M

规格：2000 Units

市场价： 2800元

High purity glucan 1,4-α-maltohexaosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.98
CAZy Family: GH13
CAS: 72561-12-7

glucan 1,4-alpha-maltohexaosidase; 4-alpha-D-glucan maltohexaohydrolase

Recombinant. From Bacillus species.
In 3.2 M Ammonium sulphate.
Supplied at ~ 500 U/mL. 

Specific activity: ~ 800 U/mg protein (on soluble starch) at pH 8.5 and 40oC.

Stability: > 4 years at 4oC.

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