转染试剂大集合

转染试剂大集合

线性PEI转染试剂简介:
线性 PEI转染试剂是一种高电荷阳离子聚合物,非常容易带负电荷的核酸分子结合,形成复合物, 并使该复合物进入细胞中。PEI转染试剂是一种瞬时转染试剂,细胞毒性低,转染效率高,在H293  CHO 等细胞中基因表达效率较高。目前已经验证线性 PEI 转染试剂广泛适用于多种细胞系。例如:H293CHO-K1COS-1COS-7NIH/3T3Sf9HepG2  Hela 细胞等。该试剂与含血清的培养基兼容,能高效的将核酸导入细胞。

上海金畔生物科技有限公司代理以下品牌的线性PEI转染试剂

品牌 产品货号 产品名称 规格
Serochem PRIME-P100-100MGPRIME-P100-1G PEI Prime粉末,转染级线性聚乙烯亚胺 100mg/1g
Prime-AQ100-5ML
Prime-AQ100-100ML
PEI Prime️ AQ 1 mg/mL 液体转染试剂 5mL/100mL
Polysciences 24765-1
24765-100
PEI MAX® – Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) 100mg/1g
23966-1
23966-100
Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K) 100mg/1g
Polyplus 114-15 jetPRIME Transfection Reagent 0.1 mL

产品展示:

       

脂质体转染试剂简介:
脂质体是磷脂分散在水中时形成的脂质双分子层,具有膜的融合及内吞作用,可用作外源物质进入细胞的载体。脂质体转染试剂原理:阳离子脂质体表层带正电荷,能与核酸的磷酸根通过静电作用将 DNA分子包裹内,形成DNA脂复合体也能被表面带负电荷的细胞膜吸附,再通过膜的融合或细胞的内吞作用,将DNA传递进入细胞由于这种转染方法具有好的重现性和较高的转染效率,不仅适用于贴壁细胞,对悬浮细胞也适用。

上海金畔生物科技有限公司代理以下品牌的脂质体转染试剂

品牌 产品货号 产品名称 规格
Polyplus 101000122 in vivo-jetRNA®+ transfection reagent 1 mL
ThermoFisher 11668019 Lipofectamine2000 transfection reagent 1.5 mL
L3000015 Lipofectamine3000 transfection reagent 1.5 mL

产品展示:

 

RNAi转染试剂简介:
阳离子脂质体转染试剂的选择对于利用siRNA介导的基因沉默实验来说同样是至关重要的。没有高效的转染,就不能有效的引发相应的细胞响应,会直接影响同一siRNA的抑制效果。只有将siRNA高效转染哺乳动物细胞,才能更真实的检测siRNA干扰基因表达的效果。如何选择zui shi he的转染试剂和转染条件,往往取决于不同的哺乳动物细胞类型和不同的核酸分子。

上海金畔生物科技有限公司代理以下品牌的RNAi转染试剂

品牌 产品货号 产品名称 规格
多纳 DN001- 01 CALNPRNAi in vitro B0.1 mL;A0.5 mL
DN001- 05 B0.5 mL;A1 mL x 2
DN001- 10 B1.0 mL;A1 mL x 4

产品展示:

人表皮角质形成细胞,成人,集合

上海金畔生物科技有限公司提供人表皮角质形成细胞,成人,集合 ,欢迎访问官网了解更多产品信息和订购。
人表皮角质形成细胞,成人,集合

详情介绍

product name:Human Epidermal Keratinocytes, Adult, Pooled(人表皮角质形成细胞,成人,集合);

cat no:ABC-TC3976;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Epidermal keratinocytes represent the major cell type of the epidermis, making up about 90% of the cells. They originate in the stratum basale and undergo gradual differentiation including profound morphological changes during their shift to the stratum corneum. In the stratum corneum, the final barrierlayer of the skin, keratinocytes are found as nucleus-free, flat, and highly keratinized squamous cells. AcceGen offers a range of Normal Human Epidermal Keratinocytes (NHEK) from single donors or pooled donors produced at Promo- Cell’s cell culture facility. The cells are isolated from juvenile foreskin or from adult normal human tissue from different locations, e.g. face, breast, abdomen, and thighs.

Shortly after isolation, all AcceGen Normal Human Epidermal Keratinocytes are cryopreserved at passage 2 (P2) using AcceGen’s proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.

Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at AcceGen.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.