人多发性骨髓瘤骨髓单核细胞(新诊断)

上海金畔生物科技有限公司提供人多发性骨髓瘤骨髓单核细胞(新诊断) ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤骨髓单核细胞(新诊断)

详情介绍

product name:Human Multiple Myeloma Bone Marrow Mononuclear Cells (Newly Diagnosed)(人多发性骨髓瘤骨髓单核细胞(新诊断));

cat no:ABC-TC3945;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-Bone Marrow-Mononuclear Cells are isolated by diluting Multiple Myeloma-bone marrow with phosphate-buffered saline and using gradient separation. Multiple Myeloma Bone Marrow-Mononuclear Cells products are available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性骨髓瘤骨髓单核细胞

上海金畔生物科技有限公司提供人多发性骨髓瘤骨髓单核细胞 ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤骨髓单核细胞

详情介绍

product name:Human Multiple Myeloma Bone Marrow Mononuclear Cells (Newly Diagnosed/Untreated)(人多发性骨髓瘤骨髓单核细胞);

cat no:ABC-TC3946;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-Bone Marrow-Mononuclear Cells are isolated by diluting Multiple Myeloma-bone marrow with phosphate-buffered saline and using gradient separation. Multiple Myeloma Bone Marrow-Mononuclear Cells products are available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性骨髓瘤骨髓血浆(新诊断/未治疗)

上海金畔生物科技有限公司提供人多发性骨髓瘤骨髓血浆(新诊断/未治疗) ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤骨髓血浆(新诊断/未治疗)

详情介绍

product name:Human Multiple Myeloma Bone Marrow Plasma (Newly Diagnosed/Untreated)(人多发性骨髓瘤骨髓血浆(新诊断/未治疗));

cat no:ABC-TC3948;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-Bone Marrow Plasma is obtained by centrifuging the Multiple Myeloma-bone marrow. The plasma has a diminished number of cells and platelets. Multiple Myeloma-Bone Marrow plasma is available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性骨髓瘤骨髓血浆(复发/难治)

上海金畔生物科技有限公司提供人多发性骨髓瘤骨髓血浆(复发/难治) ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤骨髓血浆(复发/难治)

详情介绍

product name:Human Multiple Myeloma Bone Marrow Plasma (Relapsed/Refractory)(人多发性骨髓瘤骨髓血浆(复发/难治));

cat no:ABC-TC3949;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-Bone Marrow Plasma is obtained by centrifuging the Multiple Myeloma-bone marrow. The plasma has a diminished number of cells and platelets. Multiple Myeloma-Bone Marrow plasma is available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性骨髓瘤PB血浆(新诊断/未治疗)

上海金畔生物科技有限公司提供人多发性骨髓瘤PB血浆(新诊断/未治疗) ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤PB血浆(新诊断/未治疗)

详情介绍

product name:Human Multiple Myeloma PB Plasma (Newly Diagnosed/Untreated)(人多发性骨髓瘤PB血浆(新诊断/未治疗));

cat no:ABC-TC3952;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-PB-Plasma is obtained by centrifugation of Multiple Myeloma peripheral blood. Plasma has a diminished number of cells. Multiple Myeloma-PB-Plasma is available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性骨髓瘤PB血浆(复发/难治)

上海金畔生物科技有限公司提供人多发性骨髓瘤PB血浆(复发/难治) ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤PB血浆(复发/难治)

详情介绍

product name:Human Multiple Myeloma PB Plasma (Relapsed/Refractory)(人多发性骨髓瘤PB血浆(复发/难治));

cat no:ABC-TC3953;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-PB-Plasma is obtained by centrifugation of Multiple Myeloma peripheral blood. Plasma has a diminished number of cells. Multiple Myeloma-PB-Plasma is available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性硬化性白细胞增多症

上海金畔生物科技有限公司提供人多发性硬化性白细胞增多症 ,欢迎访问官网了解更多产品信息和订购。
人多发性硬化性白细胞增多症

详情介绍

product name:Human Multiple Sclerosis Leukopak(人多发性硬化性白细胞增多症);

cat no:ABC-TC4294;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Sclerosis (MS) is a chronic disease that is often progressive. The disease is characterized by damage to the central nervous system. This includes the brain, spinal cord and the optic nerve. MS can cause numbness, severe fatigue, blurred vision and impaired speech and impaired muscular coordination.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人多发性骨髓瘤骨髓单核细胞(复发/难治)

上海金畔生物科技有限公司提供人多发性骨髓瘤骨髓单核细胞(复发/难治) ,欢迎访问官网了解更多产品信息和订购。
人多发性骨髓瘤骨髓单核细胞(复发/难治)

详情介绍

人多发性骨髓瘤骨髓单核细胞(复发/难治)

product name:Human Multiple Myeloma Bone Marrow Mononuclear Cells (Relapsed/Refractory);

cat no:ABC-TC3947;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Multiple Myeloma-Bone Marrow-Mononuclear Cells (人多发性骨髓瘤骨髓单核细胞(复发/难治))are isolated by diluting Multiple Myeloma-bone marrow with phosphate-buffered saline and using gradient separation. Multiple Myeloma Bone Marrow-Mononuclear Cells products are available in both the newly diagnosed and relapsed/refractory stages.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

多发性骨髓瘤*培养基

上海金畔生物科技有限公司提供多发性骨髓瘤*培养基 ,欢迎访问官网了解更多产品信息和订购。
多发性骨髓瘤*培养基

详情介绍

产品名称多发性骨髓瘤*培养基(Human Multiple Myeloma Cell Medium)
产品说明多发性骨髓瘤培养基(Human Multiple Myeloma Cell Medium)是一种为促进人源多发性骨髓瘤原代细胞体外生长而设计的*培养基。它是一种无菌的液体混合系统,含有必需和非必需的氨基酸、维生素、有机和无机化合物、生长因子、微量矿物质等。培养基是基于碳酸氢盐的缓冲体系,在37℃、5%CO2的培养箱中平衡时,pH值为7.4。该培养基的配方可以提供一个理想平衡的体外培养环境,选择性地促进人源多发性骨髓瘤原代细胞的生长。

产品优势
(1) 培养成功率高

(2) 体外持续扩增

(3) 保持肿瘤原始病理特征

(4) 药敏结果宇临床数据接近

使用说明:
样品分离
1)使用移液器将抗凝管中的外周血或骨髓样本混匀,转移至15mL无菌离心管中,1500rpm离心5min。
2)弃血浆,向血细胞沉淀中加入3~5倍1×PBS稀释至9mL,充分混匀。
3)另取15mL离心管,加入6mL人外周血淋巴细胞分离液,倾斜缓慢地加入上述稀释的血细胞沉淀,与分离液明显分层,设置转速400g、升速2、降速0、室温,离心30min。
4)离心后,离心管中由上至下细胞分四层(PBS层、环状乳白色白细胞层、分离液层、红细胞层),移液器环形吸取白细胞层至预先加入5mL1×PBS的15mL离心管中,轻轻混匀洗涤细胞,1500rpm室温离心3min。
5)弃上清,加入6mL红细胞裂解液重悬细胞沉淀,4℃裂解15~20min,中间颠倒混匀一次,1500rpm室温离心3min。
6)弃上清,加入1~5mL*培养基重悬细胞沉淀,活细胞计数后(细胞活率至少>50%),并参考表1,接种活细胞于培养瓶/板,置37℃、5%二氧化碳培养箱中培养。
细胞传代
1)镜下观察细胞形态,待悬浮细胞长满至80%~90%时即可传代。
2)将细胞悬液转移至15mL离心管,1500rpm室温离心3min。
3)弃上清,以1~2mL培养基重悬细胞,活细胞计数,并参考表1,接种活细胞于培养瓶/板,置37℃、5%二氧化碳培养箱中培养。
注意事项:
1.本产品在使用前需平衡至室温。
2.原代细胞培养期间尽量避免频繁摇晃,显微镜观察原代细胞状态尽量避免摇晃细胞培养器皿。
3.使用本品恶性浆细胞比例越高(至少>40%),培养成功率越高。4.使用产品时应注意无菌操作,避免污染。
5.本产品中含有血清和原代细胞专用抗生素,如无特别需要不用额外再添加血清和双抗,可直接使用。
6.为保持本产品的理想使用效果,不宜将其过夜放置于室温或较高的温度环境中。7.请于保质期内使用本产品,超出保质期,必须放弃使用。
8.本产品仅用于科研用途,不能用于体外诊断。