标签归档：mem

Gibco™ Glasgow’s MEM (GMEM)

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上海金畔生物科技有限公司提供Gibco™ Glasgow’s MEM (GMEM) ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Glasgow’s MEM (GMEM)

详情介绍

Gibco™ Glasgow's MEM (GMEM)

Using Glasgow's MEM：

Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


Dual-site cGMP manufacturing：

Glasgow's MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Glasgow's MEM product made in our Scotland facility (21710-025). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol

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上海金畔生物科技有限公司提供Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol

详情介绍

Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol red

Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts.                                                                                                       Using Opti-MEM™ Reduced-Serum Medium：

Opti-MEM™ I medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

For supply chain continuity, we manufacture Opti-MEM™ Reduced-Serum Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Gibco™ MEM, NEAA, no glutamine

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上海金畔生物科技有限公司提供Gibco™ MEM, NEAA, no glutamine ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM, NEAA, no glutamine

详情介绍

Gibco™ MEM, NEAA, no glutamine

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.


Dual site cGMP Manufacturing and Quality System：

Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ MEM product made in our Scotland facility (10370-047). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.


MEM培养基 无酚红

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上海金畔生物科技有限公司提供MEM培养基 无酚红 ，欢迎访问官网了解更多产品信息和订购。
MEM培养基 无酚红

详情介绍

Gibco™ MEM, no glutamine, no phenol red（MEM培养基 无酚红）

Using MEM：

MEM was developed by Harry Eagle, and was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM product made in our Scotland facility (51200-046). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco™ MEM α, nucleosides, powder

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上海金畔生物科技有限公司提供Gibco™ MEM α, nucleosides, powder ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM α, nucleosides, powder

详情介绍

Gibco™ MEM α, nucleosides, powder

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.


cGMP Manufacturing and Quality System：

For supply chain continuity, we manufacture Gibco™ MEM α at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.


MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.


Gibco™ MEM, powder

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上海金畔生物科技有限公司提供Gibco™ MEM, powder ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM, powder

详情介绍

Gibco™ MEM, powder

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.


Using MEM：

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).


cGMP Manufacturing and Quality System：

Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ MEM product made in our Scotland facility (61100-053). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ MEM α, no nucleosides

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上海金畔生物科技有限公司提供Gibco™ MEM α, no nucleosides ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM α, no nucleosides

详情介绍

Gibco™ MEM α, no nucleosides

Using MEM α：

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM α product made in our Scotland facility (22561-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco™ MEM α, nucleosides, GlutaMAX™ Supplement

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上海金畔生物科技有限公司提供Gibco™ MEM α, nucleosides, GlutaMAX™ Supplement ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM α, nucleosides, GlutaMAX™ Supplement

详情介绍

Gibco™ MEM α, nucleosides, GlutaMAX™ Supplement

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco™ MEM α with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.


Using MEM:

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


cGMP Manufacturing and Quality System:

Gibco™ MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ MEM α product made in our Scotland facility (32571-093). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides

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上海金畔生物科技有限公司提供Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides

详情介绍

Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides

Using MEM α:

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44- and other DHFR-negative cells. MEM α with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical MEM α product made in our Scotland facility (32561-094). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.


Gibco™ Opti-MEM™|Reduced Serum Medium

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上海金畔生物科技有限公司提供Gibco™ Opti-MEM™|Reduced Serum Medium ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Opti-MEM™|Reduced Serum Medium

详情介绍

Gibco™ Opti-MEM™|Reduced Serum Medium

Using Opti-MEM™ I Medium：

Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system：

Opti-MEM™ I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM™ I product made in our Scotland facility (31985-047). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

Gibco™ MEM, no glutamine

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上海金畔生物科技有限公司提供Gibco™ MEM, no glutamine ，欢迎访问官网了解更多产品信息和订购。
Gibco™ MEM, no glutamine

详情介绍

Gibco™ MEM, no glutamine

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.


Using MEM：

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP Manufacturing and Quality System：

Gibco™ MEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ MEM product made in our Scotland facility (21090-055). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


MEM Eagle培养基 sigma

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上海金畔生物科技有限公司提供MEM Eagle培养基 sigma ，欢迎访问官网了解更多产品信息和订购。
MEM Eagle培养基 sigma

详情介绍

Minimum Essential Medium Eagle

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:Earle’s salts (5% CO2): yes;L-glutamine: no;sodium pyruvate: no;NaHCO3: yes;phenol red: yes;HEPES: no.

General description:Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the c*tion of a wide variety of cells grown in monolayers.

Application:

Minimum Essential Medium Eagle has been used to culture:

HeLa cells;Madin-Darby canine kidney (MDCK) cells;chondrosarcoma cell line.