Thiol PEG Acid, HS-PEG-COOH Cat. No. PG2-CATH-400 400 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Thiol PEG Acid, HS-PEG-COOH

Cat. No. PG2-CATH-400 Thiol PEG Acid, HS-PEG-COOH           Cat. No. PG2-CATH-400     400 Da    100 mg
Specification 400 Da
Unit Size 100 mg
Price $485.00

Qty Add to Cart

Description:

Heterobifunctional PEG derivative that can be used to modify proteins, peptides and other materials via amino or other acid reactive chemical groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Thiol PEG Acid, HS-PEG-COOH Cat. No. PG2-CATH-20k 20000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Thiol PEG Acid, HS-PEG-COOH

Cat. No. PG2-CATH-20k Thiol PEG Acid, HS-PEG-COOH           Cat. No. PG2-CATH-20k     20000 Da    100 mg
Specification 20000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

Description:

Heterobifunctional PEG derivative that can be used to modify proteins, peptides and other materials via amino or other acid reactive chemical groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Thiol PEG Acid, HS-PEG-COOH Cat. No. PG2-CATH-5k-2 5000 Da 500 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Thiol PEG Acid, HS-PEG-COOH

Cat. No. PG2-CATH-5k-2 Thiol PEG Acid, HS-PEG-COOH           Cat. No. PG2-CATH-5k-2     5000 Da    500 mg
Specification 5000 Da
Unit Size 500 mg
Price $1,140.00

Qty Add to Cart

Description:

Heterobifunctional PEG derivative that can be used to modify proteins, peptides and other materials via amino or other acid reactive chemical groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Streptavidin, thiol function Cat. No. SV1-TH-1 1 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Streptavidin, thiol function

Cat. No. SV1-TH-1
Specification
Unit Size 1 mg
Price $485.00

Qty Add to Cart

Description:

Nanocs provides a variety of chemically functionalized and bioconjugated streptavidin with different functionality. These functional streptavidin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each streptavidin molecule.
  • streptavidin conjugates provided either in water solution or dried powder;

Storage Conditions:

  • Store 4 0C. Protect from light.

4-Arm PEG Thiol Cat. No. PG4A-TH-20k 20000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

4-Arm PEG Thiol

Cat. No. PG4A-TH-20k 4-Arm PEG Thiol           Cat. No. PG4A-TH-20k     20000 Da    100 mg
Specification 20000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

Description:

4 arm PEG derivatives have multiple reactive groups that can be used to modify proteins, peptides and other materials via their functional groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

4-Arm PEG Thiol Cat. No. PG4A-TH-10k 10000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

4-Arm PEG Thiol

Cat. No. PG4A-TH-10k 4-Arm PEG Thiol           Cat. No. PG4A-TH-10k     10000 Da    100 mg
Specification 10000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

Description:

4 arm PEG derivatives have multiple reactive groups that can be used to modify proteins, peptides and other materials via their functional groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

4-Arm PEG Thiol Cat. No. PG4A-TH-5k 5000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

4-Arm PEG Thiol

Cat. No. PG4A-TH-5k 4-Arm PEG Thiol           Cat. No. PG4A-TH-5k     5000 Da    100 mg
Specification 5000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

Description:

4 arm PEG derivatives have multiple reactive groups that can be used to modify proteins, peptides and other materials via their functional groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

4-Arm PEG Thiol Cat. No. PG4A-TH-2k 2000 Da 100 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

4-Arm PEG Thiol

Cat. No. PG4A-TH-2k 4-Arm PEG Thiol           Cat. No. PG4A-TH-2k     2000 Da    100 mg
Specification 2000 Da
Unit Size 100 mg
Price $425.00

Qty Add to Cart

Description:

4 arm PEG derivatives have multiple reactive groups that can be used to modify proteins, peptides and other materials via their functional groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces. .

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C, desiccated Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Dextran, Thiol Functional Cat. No. DX5-TH-1 5000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX5-TH-1
Specification 5000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

Dextran, Thiol Functional Cat. No. DX10-TH-1 10000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX10-TH-1
Specification 10000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

Dextran, Thiol Functional Cat. No. DX40-TH-1 40000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX40-TH-1
Specification 40000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

Dextran, Thiol Functional Cat. No. DX70-TH-1 70000 Da 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX70-TH-1
Specification 70000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

Dextran, Thiol Functional Cat. No. DX500-TH-1 500 kDa 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX500-TH-1
Specification 500 kDa
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

Dextran, Thiol Functional Cat. No. DX2000-TH-1 2000 kDa 25 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX2000-TH-1
Specification 2000 kDa
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

Dextran, Thiol Functional Cat. No. DX3-TH-1 3000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Dextran, Thiol Functional

Cat. No. DX3-TH-1
Specification 3000 Da
Unit Size 5 mg
Price $485.00

Qty Add to Cart

Description:

Thiol functional Dextran is one Nanocs premier reactive dextran conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated dextran products with molecular weight ranged from 3,000 Da to 2,000 KDa. These functional dextrans were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Dextrans are naturally synthesized polysaccharides with good water solubility, low toxicity and varied molecular weights. Dextrans modified with dyes and other biomolecules have been successfully used as long-term tracers for live cells. Nanocs also provides customerized dextran products for your special needs.

Specifications:

  • 1~50 thiol groups on each dextran molecule depends on molecular weight of dextran.
  • Dextran conjugates provided in dried powder;

Storage Conditions:

  • Store at -20 0C. Protect from light. Thiol groups may form disulfide bonds over time. In certain applications, disulfide bond reduction with DTT or TCEP may be needed.

DBCO PEG Thiol, DBCO-PEG-SH Cat. No. PG2-DBTH-3k 3400 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Thiol, DBCO-PEG-SH

Cat. No. PG2-DBTH-3k
Specification 3400 Da
Unit Size 20 mg
Price $425.00

Qty Add to Cart

Description:

DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • White solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

DBCO PEG Thiol, DBCO-PEG-SH Cat. No. PG2-DBTH-2k 2000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Thiol, DBCO-PEG-SH

Cat. No. PG2-DBTH-2k
Specification 2000 Da
Unit Size 20 mg
Price $485.00

Qty Add to Cart

Description:

DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • White solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Desthiobiotin PEG thiol Cat. No. PG2-DSBTH-5k 5000 Da 5 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Desthiobiotin PEG thiol

Cat. No. PG2-DSBTH-5k
Specification 5000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Desthiobiotin PEG Thiol:

Desthiobiotin PEG Thiol is one of Nanocs’ thiolated desthiobiotin PEG derivatives that can bind to gold particle surface with high affinity. It can also react with a number of thiol reactive groups such as maleimide, iodoacetyl and pyridyldithio, etc. Reactions bewteen thiol and gold or other groups allows conjugation of desthiobiotin tag to targeted molecule or materials substrates quickly and efficiently. Resulted desthiobiotin can be used to bind to avdidin or streptavidin. PEG linker between thiol and desthiobiotin offers better water solubility, reduced steric hindrance and ehanced reactivity.

Physical Properties:

  • Off-white/white solid;
  • Soluble in aqeous solution, DMSO or DMF;
  • Reactive group: Thiol (-SH);
  • Reactive to: Maleimide, Iodoacetyl, Pyridyldithio, etc.;

Storage Conditions:

  • Store at -20 0C. Desiccate. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

Related Products
PG2-AMDSB-5k , Desthiobiotin PEG amine

Tags: Desthiobiotin PEG,

DBCO PEG Thiol, DBCO-PEG-SH Cat. No. PG2-DBTH-1k 1000 Da 20 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Thiol, DBCO-PEG-SH

Cat. No. PG2-DBTH-1k
Specification 1000 Da
Unit Size 20 mg
Price $585.00

Qty Add to Cart

Description:

DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • White solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet

DBCO PEG Thiol, DBCO-PEG-SH Cat. No. PG2-DBTH-600 600 Da 50 mg修饰性聚乙二醇

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

DBCO PEG Thiol, DBCO-PEG-SH

Cat. No. PG2-DBTH-600
Specification 600 Da
Unit Size 50 mg
Price $850.00

Qty Add to Cart

Description:

DBCO (dibenzocycolctyne) PEG thiol (DBCO-PEG-SH) derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

  • White solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Copper-free Click reaction procedures:

  • Prepare the azide-containing reagents in reaction buffer.
  • Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
  • Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
  • Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

  • Store at -20 0C, desiccate. Protect from light. Avoid frequent thaw and freeze.
Documents
  • SDS
  • DataSheet