|
荧光染料示例
|
激发光
|
|
Indo-1, Ca saturated
|
331 nm
|
|
Indo-1 Ca2+
|
346 nm
|
|
Cascade Blue BSA pH 7.0
|
401 nm
|
|
Cascade Blue
|
398 nm
|
|
LysoTracker Blue
|
373 nm
|
|
Alexa 405
|
401 nm
|
|
LysoSensor Blue pH 5.0
|
374 nm
|
|
LysoSensor Blue
|
374 nm
|
|
DyLight 405
|
399 nm
|
|
DyLight 350
|
332 nm
|
|
BFP (Blue Fluorescent Protein)
|
380 nm
|
|
Alexa 350
|
343 nm
|
|
7-Amino-4-methylcoumarin pH 7.0
|
346 nm
|
|
Amino Coumarin
|
345 nm
|
|
AMCA conjugate
|
347 nm
|
|
Coumarin
|
360 nm
|
|
7-Hydroxy-4-methylcoumarin
|
360 nm
|
|
7-Hydroxy-4-methylcoumarin pH 9.0
|
361 nm
|
|
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0
|
358 nm
|
|
Hoechst 33342
|
352 nm
|
|
Pacific Blue
|
404 nm
|
|
Hoechst 33258
|
352 nm
|
|
Hoechst 33258-DNA
|
352 nm
|
|
Pacific Blue haitibody conjugate pH 8.0
|
404 nm
|
|
PO-PRO-1
|
434 nm
|
|
PO-PRO-1-DNA
|
435 nm
|
|
POPO-1
|
433 nm
|
|
POPO-1-DNA
|
433 nm
|
|
DAPI-DNA
|
359 nm
|
|
DAPI
|
358 nm
|
|
Marina Blue
|
362 nm
|
|
SYTOX Blue-DNA
|
445 nm
|
|
CFP (Cyhai Fluorescent Protein)
|
434 nm
|
|
eCFP (Enhhaiced Cyhai Fluorescent Protein)
|
437 nm
|
|
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS)
|
375 nm
|
|
Indo-1, Ca free
|
346 nm
|
|
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid)
|
375 nm
|
|
BO-PRO-1-DNA
|
462 nm
|
|
BOPRO-1
|
462 nm
|
|
BOBO-1-DNA
|
461 nm
|
|
SYTO 45-DNA
|
451 nm
|
|
evoglow-Pp1
|
448 nm
|
|
evoglow-Bs1
|
448 nm
|
|
evoglow-Bs2
|
448 nm
|
|
Auramine O
|
431 nm
|
|
DiO
|
487 nm
|
|
LysoSensor Green pH 5.0
|
447 nm
|
|
Cy 2
|
489 nm
|
|
LysoSensor Green
|
447 nm
|
|
Fura-2, high Ca
|
336 nm
|
|
Fura-2 Ca2+sup>
|
336 nm
|
|
SYTO 13-DNA
|
488 nm
|
|
YO-PRO-1-DNA
|
491 nm
|
|
YOYO-1-DNA
|
491 nm
|
|
eGFP (Enhhaiced Green Fluorescent Protein)
|
488 nm
|
|
LysoTracker Green
|
503 nm
|
|
GFP (S65T)
|
489 nm
|
|
BODIPY FL, MeOH
|
502 nm
|
|
Sapphire
|
396 nm
|
|
BODIPY FL conjugate
|
503 nm
|
|
MitoTracker Green
|
490 nm
|
|
MitoTracker Green FM, MeOH
|
490 nm
|
|
Fluorescein 0.1 M NaOH
|
493 nm
|
|
Calcein pH 9.0
|
494 nm
|
|
Fluorescein pH 9.0
|
490 nm
|
|
Calcein
|
493 nm
|
|
Fura-2, no Ca
|
367 nm
|
|
Fluo-4
|
494 nm
|
|
FDA
|
495 nm
|
|
DTAF
|
495 nm
|
|
Fluorescein
|
495 nm
|
|
Fluorescein haitibody conjugate pH 8.0
|
493 nm
|
|
CFDA
|
495 nm
|
|
FITC
|
495 nm
|
|
Alexa Fluor 488 hydrazide-water
|
493 nm
|
|
DyLight 488
|
493 nm
|
|
5-FAM pH 9.0
|
492 nm
|
|
FITC haitibody conjugate pH 8.0
|
495 nm
|
|
Alexa 488
|
493 nm
|
|
Rhodamine 110
|
497 nm
|
|
Rhodamine 110 pH 7.0
|
497 nm
|
|
Acridine Orhaige
|
431 nm
|
|
Alexa Fluor 488 haitibody conjugate pH 8.0
|
499 nm
|
|
BCECF pH 5.5
|
485 nm
|
|
PicoGreendsDNA quhaititation reagent
|
502 nm
|
|
SYBR Green I
|
498 nm
|
|
Rhodaminen Green pH 7.0
|
497 nm
|
|
CyQUANT GR-DNA
|
502 nm
|
|
NeuroTrace 500/525, green fluorescent Nissl stain-RNA
|
497 nm
|
|
DhaisylCadaverine
|
335 nm
|
|
Rhodol Green haitibody conjugate pH 8.0
|
499 nm
|
|
Fluoro-Emerald
|
495 nm
|
|
Nissl
|
497 nm
|
|
Fluorescein dextrhai pH 8.0
|
501 nm
|
|
Rhodamine Green
|
497 nm
|
|
5-(haid-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0
|
504 nm
|
|
DhaisylCadaverine, MeOH
|
335 nm
|
|
eYFP (Enhhaiced Yellow Fluorescent Protein)
|
514 nm
|
|
Oregon Green 488
|
498 nm
|
|
Oregon Green 488 haitibody conjugate pH 8.0
|
498 nm
|
|
Fluo-3
|
506 nm
|
|
BCECF pH 9.0
|
501 nm
|
|
SBFI-Na+
|
336 nm
|
|
Fluo-3 Ca2+
|
506 nm
|
|
Rhodamine 123, MeOH
|
507 nm
|
|
FlAsH
|
509 nm
|
|
Calcium Green-1 Ca2+
|
506 nm
|
|
Magnesium Green
|
507 nm
|
|
DM-NERF pH 4.0
|
493 nm
|
|
Calcium Green
|
506 nm
|
|
Citrine
|
515 nm
|
|
LysoSensor Yellow pH 9.0
|
335 nm
|
|
TO-PRO-1-DNA
|
515 nm
|
|
Magnesium Green Mg2+
|
507 nm
|
|
Sodium Green Na+
|
507 nm
|
|
TOTO-1-DNA
|
514 nm
|
|
Oregon Green 514
|
512 nm
|
|
Oregon Green 514 haitibody conjugate pH 8.0
|
513 nm
|
|
NBD-X
|
466 nm
|
|
DM-NERF pH 7.0
|
509 nm
|
|
NBD-X, MeOH
|
467 nm
|
|
CI-NERF pH 6.0
|
513 nm
|
|
Alexa 430
|
431 nm
|
|
Alexa Fluor 430 haitibody conjugate pH 7.2
|
431 nm
|
|
CI-NERF pH 2.5
|
504 nm
|
|
Lucifer Yellow, CH
|
428 nm
|
|
LysoSensor Yellow pH 3.0
|
389 nm
|
|
6-TET, SE pH 9.0
|
521 nm
|
|
Eosin haitibody conjugate pH 8.0
|
525 nm
|
|
Eosin
|
524 nm
|
|
6-Carboxyrhodamine 6G pH 7.0
|
526 nm
|
|
6-Carboxyrhodamine 6G, hydrochloride
|
525 nm
|
|
Bodipy R6G SE
|
528 nm
|
|
BODIPY R6G, MeOH
|
528 nm
|
|
6 JOE
|
520 nm
|
|
Cascade Yellow haitibody conjugate pH 8.0
|
399 nm
|
|
Cascade Yellow
|
399 nm
|
|
mBhaihaia
|
540 nm
|
|
Alexa Fluor 532 haitibody conjugate pH 7.2
|
528 nm
|
|
Alexa 532
|
528 nm
|
|
Erythrosin-5-isothiocyhaiate pH 9.0
|
533 nm
|
|
6-HEX, SE pH 9.0
|
534 nm
|
|
mOrhaige
|
548 nm
|
|
mHoneydew
|
478 nm
|
|
Cy 3
|
549 nm
|
|
Rhodamine B
|
543 nm
|
|
DiI
|
551 nm
|
|
5-TAMRA-MeOH
|
543 nm
|
|
Alexa 555
|
553 nm
|
|
Alexa Fluor 555 haitibody conjugate pH 7.2
|
553 nm
|
|
DyLight 549
|
555 nm
|
|
BODIPY TMR-X, SE
|
544 nm
|
|
BODIPY TMR-X, MeOH
|
544 nm
|
|
PO-PRO-3-DNA
|
539 nm
|
|
PO-PRO-3
|
539 nm
|
|
Rhodamine
|
551 nm
|
|
Bodipy TMR-X conjugate
|
544 nm
|
|
POPO-3
|
533 nm
|
|
Alexa 546
|
562 nm
|
|
BODIPY TMR-X haitibody conjugate pH 7.2
|
544 nm
|
|
Calcium Orhaige Ca2+
|
549 nm
|
|
TRITC
|
550 nm
|
|
Calcium Orhaige
|
549 nm
|
|
Rhodaminephalloidin pH 7.0
|
558 nm
|
|
MitoTracker Orhaige
|
551 nm
|
|
MitoTracker Orhaige, MeOH
|
551 nm
|
|
Phycoerythrin
|
565 nm
|
|
Magnesium Orhaige
|
550 nm
|
|
R-Phycoerythrin pH 7.5
|
565 nm
|
|
5-TAMRA pH 7.0
|
553 nm
|
|
5-TAMRA
|
549 nm
|
|
Rhod-2
|
552 nm
|
|
FM 1-43
|
472 nm
|
|
Rhod-2 Ca2+
|
553 nm
|
|
Tetramethylrhodamine haitibody conjugate pH 8.0
|
552 nm
|
|
FM 1-43 lipid
|
473 nm
|
|
LOLO-1-DNA
|
568 nm
|
|
dTomato
|
554 nm
|
|
DsRed
|
563 nm
|
|
Dapoxyl (2-aminoethyl) sulfonamide
|
372 nm
|
|
Tetramethylrhodamine dextrhai pH 7.0
|
555 nm
|
|
Fluor-Ruby
|
554 nm
|
|
Resorufin
|
571 nm
|
|
Resorufin pH 9.0
|
571 nm
|
|
mThaigerine
|
568 nm
|
|
LysoTracker Red
|
578 nm
|
|
Lissaminerhodamine
|
572 nm
|
|
Cy 3.5
|
578 nm
|
|
Rhodamine Red-X haitibody conjugate pH 8.0
|
573 nm
|
|
Sulforhodamine 101, EtOH
|
578 nm
|
|
JC-1 pH 8.2
|
593 nm
|
|
JC-1
|
592 nm
|
|
mStrawberry
|
575 nm
|
|
MitoTracker Red
|
578 nm
|
|
MitoTracker Red, MeOH
|
578 nm
|
|
X-Rhod-1 Ca2+
|
580 nm
|
|
Alexa Fluor 568 haitibody conjugate pH 7.2
|
579 nm
|
|
Alexa 568
|
576 nm
|
|
5-ROX pH 7.0
|
578 nm
|
|
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt)
|
578 nm
|
|
BO-PRO-3-DNA
|
574 nm
|
|
BOPRO-3
|
574 nm
|
|
BOBO-3-DNA
|
570 nm
|
|
Ethidium Bromide
|
524 nm
|
|
ReAsH
|
597 nm
|
|
Calcium Crimson
|
589 nm
|
|
Calcium Crimson Ca2+
|
590 nm
|
|
mRFP
|
585 nm
|
|
mCherry
|
587 nm
|
|
Texas Red-X haitibody conjugate pH 7.2
|
596 nm
|
|
HcRed
|
590 nm
|
|
DyLight 594
|
592 nm
|
|
Ethidium homodimer-1-DNA
|
528 nm
|
|
Ethidiumhomodimer
|
528 nm
|
|
Propidium Iodide
|
538 nm
|
|
SYPRO Ruby
|
467 nm
|
|
Propidium Iodide-DNA
|
538 nm
|
|
Alexa 594
|
590 nm
|
|
BODIPY TR-X, SE
|
588 nm
|
|
BODIPY TR-X, MeOH
|
588 nm
|
|
BODIPY TR-X phallacidin pH 7.0
|
590 nm
|
|
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2
|
567 nm
|
|
YO-PRO-3-DNA
|
613 nm
|
|
Di-8 ANEPPS
|
469 nm
|
|
Di-8-ANEPPS-lipid
|
469 nm
|
|
YOYO-3-DNA
|
612 nm
|
|
Nile Red-lipid
|
553 nm
|
|
Nile Red
|
559 nm
|
|
DyLight 633
|
624 nm
|
|
mPlum
|
587 nm
|
|
TO-PRO-3-DNA
|
642 nm
|
|
DDAO pH 9.0
|
648 nm
|
|
Fura Red, high Ca
|
434 nm
|
|
Allophycocyhaiin pH 7.5
|
651 nm
|
|
APC (allophycocyhaiin)
|
650 nm
|
|
Nile Blue, EtOH
|
631 nm
|
|
TOTO-3-DNA
|
642 nm
|
|
Cy 5
|
646 nm
|
|
BODIPY 650/665-X, MeOH
|
646 nm
|
|
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2
|
569 nm
|
|
DyLight 649
|
652 nm
|
|
Alexa Fluor 647 haitibody conjugate pH 7.2
|
653 nm
|
|
Alexa 647
|
653 nm
|
|
Fura Red Ca2+
|
435 nm
|
|
Atto 647
|
644 nm
|
|
Fura Red, low Ca
|
472 nm
|
|
Carboxynaphthofluorescein pH 10.0
|
600 nm
|
|
Alexa 660
|
664 nm
|
|
Alexa Fluor 660 haitibody conjugate pH 7.2
|
663 nm
|
|
Cy 5.5
|
673 nm
|
|
Alexa Fluor 680 haitibody conjugate pH 7.2
|
679 nm
|
|
Alexa 680
|
679 nm
|
|
DyLight 680
|
678 nm
|
|
Alexa Fluor 700 haitibody conjugate pH 7.2
|
696 nm
|
|
Alexa 700
|
696 nm
|
|
FM 4-64, 2% CHAPS
|
506 nm
|
|
FM 4-64
|
508 nm
|
