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Dulbecco′s Modified Eagle′s Medium – high glucose

This Dulbecco′s Modified Eagle′s Medium – high glucose:

sterility:sterile-filtered.

form:liquid.

components:glucose: high;sodium pyruvate: yes;HEPES: no;NaHCO3: yes;L-glutamine: yes;phenol red: yes.General description:This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.Application:Dulbecco′s Modified Eagle′s Medium – high glucose has been used for cell culture.

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name “DMEM/High”.