Agarose, S3 high capacity acyl-RAC capture beads
Similar products: Activated thiol Sepharose, activated thiol agarose, thiopropyl Sepharose 6B, thiopropyl activated agarose.
Applications: acyl-RAC protein assays; Protein pamitoylation; Site-specific protein immobilization; Protein purification.
High capacity thiol capture,S3TM Beads are one of Nanocs reactive agarose beads that can be used for reversible immobilization of biomolecules containing free thiol groups. S3TM thiol capture beads contain reactive pyridyl disulfide bonds that can react easily with free thiol groups under mild conditions. Reaction between thiol and pyridyl disulfide results a reversible dithio bond that can be cleaved by common dithio reducing agents such as DTT or TCEP. Compared to other thiol reactive beads, Nanocs S3TM Beads have exceptionally high thiol capture capacity; Density of thiol reactive groups on S3 beads is 20-30 times more over conventional thiol activated agarose beads. S3 beads can be used in many applications, such as acyl-RAC protein assays; Protein pamitoylation research; Site-specific protein immobilizations; Protein purification, digestion and analysis. S3 beads are ideal replacements for commonly used activated Thiol-Sepharose 4B or Thiopropyl Sepharose 6B from GE.
- High thiol binding capacity;
- Reversible disulfide linkage;
- Low non-specific binding;
- Bead Matrix: 6B pegylated agarose beads;
- Bead Size: 50~150 microns;
- Ligand type: Reactive dithiopyridine;
- Ligand density: 800-1000 umol/g beads;
- Reactive to: Free sulfhydryl/thiol groups (cystein, etc.);
- Form: 50% aqueous suspension.
- Store at 4-8 0C.
Acyl-rac Related Reagents:
- DTT (Dithiothreitol)
- Ellman's reagent
- mPEG Maleimide
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|AR-S3-2 , Agarose, S3 high capacity acyl-rac capture beads, 20 mL|