N-ethylmaleimide (NEM) is a water soluble, thiol reactive maleimide that can be used to modify protein cysteine residues. Maleimide reacts with sulfhydryl groups at pH 6.5~7.5 to form stable thioether bonds. NEM has been used for protein Acyl-RAC assay. N-ethylmaleimide is a sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC50 = 22 µM) in pancreatic duct cells. N-ethylmaleimide Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei. NEM stimulates arachidonic acid release through activation of PLA2 in endothelial cells. As a commonly used sulfhydryl alkylating reagent, NEM has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei.
- Appearance: Off-white/faint yellow solid
- Mol Wt: 125.13
- CAS No: 128-53-0
- Purity: >98%
- Store at -20 0C.
|AR-SS-1 , Agarose, S3 high capacity acyl-RAC capture beads|
|AR-SR-1 , Thiol reducing Agarose beads|