Dionex Acclaim 300 C18 Column 生物色谱柱


Dionex Acclaim 300 C18 Column 生物色谱柱

简要描述:

戴安公司生产的仪器主要有离子色谱仪、高效液相色谱仪、快速溶剂萃取仪和氨基酸直接分析仪。其中离子色谱仪凭借着雄厚的技术优势和方法已广泛地应用于各个领域,其市场占有率达80%。与离子色谱相关的文章有90%均出自戴安公司生产的离子色谱仪。

型号 美国戴安 P/N: 060265

Acclaim 300 C18 Protein and Peptide Column

High-Resolution Separation of Proteins and Peptides

The Acclaim? 300 features 3 ?m silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.

  • Designed for high-resolution peptide mapping and protein separations
  • High-efficiency 3 ?m spherical silica substrate
  • High-performance bonding chemistry on 300 ? pore silica
  • Application tested for suitablility in peptide mapping
  • Reproducible for dependable results
  • LC/MS compatible

Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. 

The use of a 3 ?m silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 ?m column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.

Acclaim PepMap for TFA-Free Peptide Mapping  Specifications
Column type Nano Capillary Micro
Column i.d. 75 ?m 300 ?m 1.0 mm
Length 50, 150, 250, and 500 mm 50, 150, and
250 mm
50, 150, and
250 mm
Recommended Flow Rate 200 nL/min 4 ?L/min 40 ?L/min
Packing Material C18, 100 ?,
3 ?m
C18, 100 ?,
3 ?m
C18, 100 ?,
3 ?m
C18, 300 ?,
5 ?m
C18, 300 ?,
5 ?m
C18, 300 ?,
5 ?m
C8, 100 ?,
3 ?m 
C8, 100 ?,
3 ?m
C8, 100 ?,
3 ?m
C8, 100 ?,
5 ?m 
C8, 100 ?,
5 ?m
C8, 100 ?,
5 ?m
C4, 300 ?,
5 ?m
C4, 300 ?,
5 ?m
C4, 300 ?,
5 ?m
Column Fused-silica Glass Lined Tubing Stainless steel
Column Inlet 1/16-in.
PEEK sleeve adapter
(provided)
nanoViper (optional)
1/16-in.
universal fitting
nanoViper (optional) 50?m i.d. PEEK sheathed fused silica with nanoViper fittings
1/16-in.
PEEK tubing
nanoViper (optional) 75?m i.d. PEEK sheathed fused silica with nanoViper fittings
Column Outlet Fused-silica
25-?m i.d./ 280-?m o.d.
Fused-silica
75-?m i.d./ 280-?m o.d
1/16-in.
PEEK tubing
nanoViper (optional) 75?m i.d. PEEK sheathed fused silica with nanoViper fittings
MS Outlet 1-m Length

The standard particle size is nominally 3 ?m. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters; standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 ?m, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.

Analytical Columns
Acclaim 300, C18, 3 ?m Analytical (2.1 x 50 mm) 060263
Acclaim 300, C18, 3 ?m Analytical (2.1 x 150 mm) 060264
Acclaim 300, C18, 3 ?m (3.0 x 150 mm) 063684
Acclaim 300, C18, 3 ?m Analytical (4.6 x 50 mm) 060265
Acclaim 300, C18, 3 ?m Analytical (4.6 x 150 mm) 060266
Guard Columns
Acclaim 300,C18, 3?m, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 069690
PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA 069697
Hardware
Acclaim SST Guard Cartridge Holder V-1 059456
Guard to Analytical Column Coupler 059457
Guard Kit (Holder and Coupler) 059526
Acclaim SST Guard Cartridge Holder V-2 069580
Acclaim Guard Kit (Holder and coupler) V-2 069707
Guard to Analytical Column Coupler V-2 074188
Micro and Nano Columns
Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper 164567
75 ?m i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 ?m, 300?, nanoViper 164708
300 ?m i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 ?m, 100?, nanoViper 164716
300 ?m i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 ?m, 300?, nanoViper 164718
Acclaim PepMap300, C18, 5 ?m, 300 ? Wide Pore, 1.0 mm x 5 cm 163584
Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper 164572