Dionex GlycanPac AXH-1 Column GlycanPac系列生物色谱柱

Dionex GlycanPac AXH-1 Column GlycanPac系列生物色谱柱



型号 美国戴安 P/N: 082469

GlycanPac AXH-1 Glycan Analysis Column

GlycanPac AXH-1 Columns for the High-Resolution Separation and Analysis of Glycans

The Thermo Scientific? GlycanPac? AXH-1 column is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.

The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules (e.g., proteins).


  • Unique glycan selectivity based on charge, size, and polarity
  • Excellent resolution for both native and labeled glycans
  • Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
  • Compatible with fluorescence and MS detection methods
  • High chromatographic efficiency and excellent column stability

The GlycanPac AXH-1 column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans, while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 column provides unparalleled capabilities for glycan separations. Additionally, this column offers charge-based separations for various glycans without discrimination of size and polarity, making it a suitable tool for accurate quantification of glycans which cannot be achieved with other HPLC columns. The GlycanPac AXH-1 column is designed and tested for LC-fluorescence detection and LC-MS applications using volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile.

GlycanPac AXH-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 ?m sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXH-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.

GlycanPac AXH-1 Column Specifications
Column Dimensions

1.9 μm: 2.1 × 250mm, 2.1 × 150mm, 2.1 ×100mm

3 μm: 4.6 × 150mm, 3.0 × 150mm, 2.1 × 150mm

Bead Size Spherical 1.9 μm, 3 μm particles
Substrate High-purity, Porous, Spherical Silica
Functional Group WAX and HILIC Mixed-Mode
Recommended Flow Rate Range:

1.9 μm: 0.1 to 0.4 mL/min. for:  all 2.1 mm columns

3 μm: 0.6 to 1.2 ml/min  for 4.6  × 150 mm 

          0.3 to 0.6 ml/min  for 3.0  × 150 mm  

          0.1 to 0.5 ml/min. for 2.1 × 150 mm

Pressure Limit

1.9 μm: 7,000 psi for 2.1 × 100mm,

             10,000 psi for 2.1 ×150mm

             15,000 psi for 2.1 × 250mm

3 μm: 6,000 psi for all 3 μm columns

Temperature Range: Ambient to 60  ?C
pH Range 2.0 – 8.0

Analytical Columns
GlycanPac AXH-1, 3 ?m, Analytical (4.6x150mm) 082468
GlycanPac AXH-1, 3 ?m, Analytical (3.0x150mm) 082469
GlycanPac AXH-1, 3 ?m, Analytical (2.1x150mm) 082470
GlycanPac AXH-1, 1.9 ?m, Analytical (2.1x250mm) 082521
GlycanPac AXH-1, 1.9 ?m, Analytical (2.1x150mm) 082472
GlycanPac AXH-1, 1.9 ?m, Analytical (2.1x100mm) 082473
GlycanPac AXH-1, 1.9 ?m, Analytical (1.0 × 150 mm, with nanoViper) 164863
Guard Columns
GlycanPac AXH-1, 3 ?m, Guard (4.6×10 mm) 2ea (Use Holder V-2) 082474
GlycanPac AXH-1, 3 ?m, Guard (3.0×10 mm) 2ea (Use Holder V-2) 082475
GlycanPac AXH-1, 3 ?m, Guard (2.1×10 mm) 2ea (Use Holder V-2) 082476
Acclaim SST Guard Cartridge Holder V-2 069580
Acclaim Guard Kit (Holder and coupler) V-2 069707
Guard to Analytical Column Coupler V-2 074188