Dionex AminoPac PA1 Amino Acid Column 生物色谱柱

Dionex AminoPac PA1 Amino Acid Column 生物色谱柱



型号 美国戴安 P/N: 037023

AminoPac PA1 Amino Acid Column

For Specialized Amino Acid Applications with Postcolumn Derivatization

The AminoPac? PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead? particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

  • Resolves phosphorylated and other amino acids by anion-exchange chromatography
  • Separates acid-labile amino acids under basic pH conditions
  • Separates strongly acidic amino acids
  • Requires OPA or ninhydrin postcolumn derivatization

The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not compley resolved by cation-exchange chromatography.

AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization  Specifications
Dimensions AminoPac PA1 Analytical Column:
4 × 250 mm
AminoPac PA1 Guard Column:
4 × 50 mm
Maximum operating pressure 4000 psi (27.9 MPa)
Recommended operating temperature Ambient
Typical operating conditions 1.0 mL/min gradient
Recommended flow rate: 1.0 mL/min
phase compatibility Compatible with up to 5% common organic solvents.
Recommended eluents: hydroxide, borate, and acetate.
Resin composition 10-μm-diameter, pellicular substrate (2% cross-linked)
agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
Anion-exchange capacity N/A
Column construction PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
Analytical Columns
AminoPac PA1 Analytical Column (4 x 250 mm) 037022
Guard Columns
AminoPac PA1 Guard Column (4 x 50 mm) 037023