New England Biolabs supplies a 10X reaction buffer for use with NEBNext^® End Repair Enzyme Mix.

At a 1X concentration this reaction buffer assures optimal activity of the enzyme mix.

1X Buffer Components

50 mM Tris-HCl 
10 mM MgCl₂ 
10 mM DTT 
1 mM ATP 
0.4 mM dATP 
0.4 mM dCTP 
0.4 mM dGTP 
0.4 mM dTTP 
pH 7.5@25°C

-20°C

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